Starch

Copper(II) sulphate pentahydrate, citric acid monohydrate, anhydrous sodium carbonate, potassium hexacyanoferrate(II) trihydrate, zinc acetate, calcium carbonate, potassium iodide, sulphuric acid, potassium thiocyanate, starch, phenolphthalein, sodium hydroxide, sodium thiosulfate and sodium chloride were purchased from Kemika (Zagreb, Croatia). Myrtenol standard was obtained from Sigma Aldrich (St. Lois, MO, USA) and C7-C30 saturated alkanes standard from Merck (Darmstadt, Germany). Helium for gas chromatography was obtained from Messer, Austria.

All standards of fatty acid methyl esters were purchased from Sigma (St. Louis, MO, USA). Methanol, n-hexane, petroleum ether, chloroform, potassium iodide, Folin–Ciocalteu reagent, sodium carbonate, starch, concentrated sulphuric, nitric and hydrochloric acid (37%) were purchased from Kemika (Zagreb, Croatia). Anhydrous sodium sulphate and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) were purchased from Sigma (St. Louis, MO, USA).
Sodium and potassium hydroxides in the form of pellets, chloroform and analytical standards (dihydrocholesterol, campesterol, stigmasterol, β-sitosterol, eicosanol, docosanol, tricosanol, tetracosanol, pentacosanol, hexacosanol, heptacosanol, octacosanol, squalene and (±)-α-tocopherol) were purchased from Sigma (St. Louis, MO, USA). Ethanol and a silylating mixture according to Sweeley were purchased from Fluka (Buchs, Switzerland).
Hydroxytyrosol, tyrosol, oleuropein, lutein and apigenin were purchased from Extrasynthese (Genay, France). Caffeic acid and gallic acid were purchased from Sigma (St. Louis, MO, USA). Standard stock solutions of each compound were 1 mg/mL in Methanol.
All mineral standards were purchased from Merck (Darmstadt, Germany) and diluted to the appropriate obtained concentration.
All standards stored at +4 °C in amber vials and in a dark place until analysis.