Human HAP1 cells (Horizon Discovery) were cultured in IMDM (Gibco) supplemented with 10% FBS (Corning) at 37°C and 5% CO2 in a humidified incubator, with daily media change. Cells were split every 2 to 3 days using TrypLE Express (Gibco).
Human hap1 cells
Manufactured by Horizon Discovery
Sourced in United Kingdom
The Human HAP1 cells are a near-haploid human cell line derived from the chronic myelogenous leukemia cell line KBM-7. These cells have a karyotype of 23 chromosomes, making them a useful tool for genetic studies and research requiring a simplified genomic background.
Automatically generated - may contain errors
Lab products found in correlation
Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (3 mentions)
Tryple express, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Corning (2 mentions)
Dulbecco modified eagle medium (dmem), by Merck Group (1 mentions)
Chymotrypsin, by Promega (1 mentions)
Modified porcine trypsin, by Promega (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Mm hepes, by Thermo Fisher Scientific (1 mentions)
Sucrose, by Merck Group (1 mentions)
4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes, by Merck Group (1 mentions)
Ammonium bicarbonate, by Merck Group (1 mentions)
Iscove s modified dulbecco s medium, by Thermo Fisher Scientific (1 mentions)
6 protocols using human hap1 cells
1
Culturing HAP1 Cells in Supplemented IMDM
2
Cell Culture Conditions and Mycoplasma Testing
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Human HAP1 cells (Horizon Discovery) were cultured in IMDM and HEK293 and 293T/17 cells (ATCC) were cultured in DMEM, each supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 g/mL streptomycin. During routine passaging, cells were washed with PBS (pH 7.4) and dissociated with TrypLE Express. Cell lines were tested monthly for mycoplasma; all were negative.
3
Cell Culture Protocols for Diverse Cell Lines
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Human HAP1 cells were obtained from Horizon Discovery, they were grown in Iscove’s Modified Dulbecco’s Medium (IMDM) from GIBCO®, containing l -Glutamine and 25 mM HEPES and supplemented with 10% fetal bovine serum (FBS) and 1% Penicillin/Streptomycin (P/S). HEK293T cells were obtained from the CRUK Cell Facility, they were used for virus production, expanded in Dulbecco’s modified Eagle medium (DMEM) and supplemented with 10% FBS. U2OS cells were originally obtained from ATCC cell repository, they were cultured in DMEM (Sigma-Aldrich), supplemented with 10% FBS. Human mCherry-Geminin-expressing U2OS44 (link), FANCC-deficient VU1131 and complemented VU1131 cells (a gift from Josephine Dorsman, VU Medical Center Amsterdam) were cultured in DMEM, supplemented with antibiotics and 10% fetal calf serum. VU1131 cells were grown with G418 (300 μg/mL). All cells were grown at 37 °C in a 3% oxygen and 5% CO2 atmosphere. All cell lines used in this publication were tested negative for mycoplasma contamination using the MycoAlert™ Mycoplasma Detection Kit. They were all authenticated by the specified providers and, furthermore, they are not listed as commonly misidentified by ICLAC.
4
Culturing HAP1 Cells in Supplemented IMDM
5
Proteomic analysis of HAP1 cells
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All of the chemicals were of the highest purity commercially available and used without further purification. Ammonium bicarbonate, calcium chloride, phosphate buffered saline (PBS), Tris-HCl, Triton X-100, sucrose, mannitol, ethylene glycol tetraacetic acid (EGTA), ethylenediaminetetraacetic acid (EDTA), 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), formic acid (FA), dithiothreitol (DTT), iodoacetamide (IAA), and fetal bovine serum (FBS) were obtained from Sigma–Aldrich (Milan, Italy). IMDM (Iscove’s Modified Dulbecco’s Medium containing L-glutamine and 25 mM HEPES) was obtained from Gibco-Thermo Fisher Scientific. Human HAP1 cells were from Horizon Discovery (Cambridge, United Kingdom UK). Modified porcine trypsin and chymotrypsin were purchased from Promega (Milan, Italy). Water and acetonitrile (OPTIMA® LC/MS grade) for LC/MS analyses were provided from Fisher Scientific (Milan, Italy).
6
Cell Culture Conditions for HAP1 and HEK293T
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Human HAP1 cells were obtained from Horizon Discovery and were grown in Iscove’s Modified Dulbecco’s Medium (IMDM) from GIBCO®, containing L-Glutamine and 25 mM HEPES and supplemented with 10% Fetal Bovine Serum (FBS) and 1% Penicillin/Streptomycin (P/S). All cell lines were diploid at the time of the experiments. HEK293T cells were obtained from the CRUK Cell Facility and were used for virus production, by culturing in Dulbecco’s modified Eagle medium (DMEM) and supplemented with 10% FBS.
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