Pstat 4 clone 38

Manufactured by BD

The PSTAT-4 (clone 38) is a laboratory instrument designed for electrochemical analysis. It is capable of performing potentiostatic and galvanostatic measurements. The device provides precise control and monitoring of electrochemical parameters such as potential, current, and charge.

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Lab products found in correlation

Phosphoflow fix buffer 1, by BD (1 mentions) Ifn γ clone 4s b3, by BD (1 mentions) Enhanced chemiluminescence detection system, by GE Healthcare (1 mentions) Horseradish peroxidase coupled secondary antibody, by GE Healthcare (1 mentions) Rabbit anti β actin polyclonal antibody, by Abcam (1 mentions)

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PSTAT-4

2 protocols using pstat 4 clone 38

Flow Cytometry Analysis of Human Cells

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The following antibodies were used to perform flow cytometry on human cells (all antibodies are from Biolegend unless indicated otherwise): ARG1 (clone 14D2C43), CCR7 (clone G043H7), anti-CD3 (clone RPA-T4), CD4 (clone A161A1), CD8 (clone SK1), CD45RA (clone H1100), CD45RO (clone UCHL1), IFN-γ (clone 4S.B3), IL-10 (clone JES-9D7), IL-4 (clone MP4–25D2), IL-17A (clone BL168), TNFa (Mab11), Ki67 (clone B56, BD biosciences), pSTAT-4 (clone 38, BD Biosciences), pSTAT-1 (clone 4a, BD Biosciences).
Cell surface CAT-1 detection was performed using a soluble ligand derived from the receptor-binding domain (RBD) of the bovine leukemia virus (BLV) envelope glycoprotein (BLV-RBD).^{66 (link)} Staining was realized with a rabbit Fc-tagged BLV-RBD (BLV.RBD.rabbitIgG1-Fc) (Metafora-biosystems), followed by an anti-rabbit IgG1-Fc secondary antibody (Thermo Fisher), as previously described.^{66 (link)}For intracellular staining, BDcytofix/cytoperm buffers were used, except for FOXP3 staining (#00–5523-00, Thermo Fisher) and for pSTAT-1 and pSTAT-4 (# 557870, BD Biosciences phosphoflow fix buffer I and #554656, BD Biosciences phosphoflow perm buffer III) were used according to manufacturer’s protocol.

West E.E., Merle N.S., Kamiński M.M., Palacios G., Kumar D., Wang L., Bibby J.A., Overdahl K., Jarmusch A.K., Freeley S., Lee D.Y., Thompson J.W., Yu Z.X., Taylor N., Sitbon M., Green D.R., Bohrer A., Mayer-Barber K.D., Afzali B., Kazemian M., Scholl-Buergi S., Karall D., Huemer M, & Kemper C. (2023). Loss of CD4+ T cell-intrinsic arginase 1 accelerates Th1 response kinetics and reduces lung pathology during influenza infection. Immunity, 56(9), 2036-2053.e12.

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Western Blot Analysis of STAT4 and pSTAT4

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Total splenic leukocytes and FACS-sorted CD8, LCMV-specific (LCMV-Tet⁺) CD8, and nonspecific (LCMV-Tet⁻) CD8 T cells were prepared and lysed for Western blot analysis as previous described (11 (link)). Mouse monoclonal antibodies against STAT4 (clone 8) and pSTAT4 (clone 38) were purchased from BD Biosciences. A rabbit anti-pSTAT4 polyclonal antibody was also used. A rabbit anti-β-actin polyclonal antibody was obtained from Abcam (Cambridge, United Kingdom). Proteins were resolved by SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and then incubated with antibodies. Reactive bands on Western blots were detected with horseradish peroxidase-coupled secondary antibodies and an enhanced chemiluminescence detection system (GE Healthcare). Anti-STAT4 and -pSTAT4 antibodies were loaded. A rabbit anti-β-actin polyclonal antibody from Abcam was used as a loading control. Control samples were prepared from STAT4-deficient (9 (link)) and STAT1-deficient (50 (link)) mice.

Suarez-Ramirez J.E., Tarrio M.L., Kim K., Demers D.A, & Biron C.A. (2014). CD8 T Cells in Innate Immune Responses: Using STAT4-Dependent but Antigen-Independent Pathways to Gamma Interferon during Viral Infection. mBio, 5(5), e01978-14.

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