Anti ecp

Manufactured by Biorbyt

Sourced in United Kingdom

Anti-ECP is a laboratory product used to detect and quantify the presence of Eosinophil Cationic Protein (ECP) in biological samples. ECP is a protein released by eosinophils, a type of white blood cell, and is considered a biomarker for various inflammatory conditions. The Anti-ECP product provides researchers and clinicians with a tool to measure ECP levels, which can aid in the diagnosis and monitoring of disorders involving eosinophil activation and inflammation.

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Lab products found in correlation

Mbd2 antibody, by Abcam (4 mentions) Anti gr1, by BioLegend (4 mentions) Ovalbumin (ova), by Merck Group (2 mentions) Bicinchoninic acid (bca), by Beyotime (1 mentions) Max 1320, by Buxco Electronics (1 mentions) Pan cytokeratin, by Santa Cruz Biotechnology (1 mentions) Fixable viability stain 510 antibody, by BD (1 mentions) Application suite v4, by Leica (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Anti il 4 pe, by BD (1 mentions) Anti il 17a apc, by BioLegend (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Aluminum hydroxide gel, by Merck Group (1 mentions) Anti tlr 7, by Cell Signaling Technology (1 mentions) Dylight 800 680 secondary antibodies, by Thermo Fisher Scientific (1 mentions) Mouse tlr 1 9 agonist kit, by InvivoGen (1 mentions) Anti tlr7, by Abcam (1 mentions) Murine il 5, by Thermo Fisher Scientific (1 mentions) Anti β actin, by Merck Group (1 mentions) Anti tlr7, by Thermo Fisher Scientific (1 mentions)

5 protocols using anti ecp

Asthma Mouse Model Reagents

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GREER laboratories supplied HDM; OVA, LPS, and aluminum hydroxide gel from Sigma; mice spirometer (MAX 1320) from Buxco®, USA; Beijing Zhongshidichuang Science and Technology Development Co. Ltd. supplied animal asthma inducing instrument (YLS-8A); centrifuge (5810 R) from Eppendorf®, Germany; image analyzer (Leica Application Suite V4) from Leica, Germany; and Olympus, Japan (Leica®) supplied optical microscope (DMI3000B); 150-mesh cell sieve (Biosharp, BS-100-XBS, China); bronchial epithelial growth medium (Procell, CM-M007, China); DAPI and cytokeratin specific monoclonal antibody (pan-Cytokeratin, Santa Cruz, sc-8018, USA); leukocyte activation cocktail (550583, BD Biosciences, USA); cell viability marker (Fixable Viability Stain 510 antibody, BD Pharmingen); PE-anti-IL-4 (BD Pharmingen); APC-anti-IL-17A (Biolegend); Lipofectamine 3000 (Invitrogen, USA); bicinchoninic acid (BCA), Beyotime, Shanghai, China; DHT (B8214) and 17β-estradiol (C4348) APExBIO, USA; MBD2 antibody (Abcam, Cambridge, USA); eosinophil antibody (anti-ECP, Biorbyt, Cambridge, UK); neutrophil antibody (anti-Gr-1, Biolegend, San Diego, USA); GATA3 (10417-1-AP) and β actin (60008-1-Ig) Proteintech, USA; RORγt (EPR20006) abcam, USA.

Wasti B., Chen Z., Yuan Y., He Y., Duan W., Jia J., Li D., Xiao B., Li J., Liu Y., Liu S., Zhang D., Zhang X., Ma L, & Xiang X. (2022). Androgen Plays a Potential Novel Hormonal Therapeutic Role in Th17 Cells Predominant Neutrophilic Severe Asthma by Attenuating BECs Regulated Th17 Cells Differentiation via MBD2 Expression. Oxidative Medicine and Cellular Longevity, 2022, 3096528.

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Lung Histopathological Analysis

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The lungs were fixed with 10% formalin via the trachea, then removed and stored in 10% formalin. Fixed lung tissues were paraffinized and sectioned (5 μm) for hematoxylin and eosin (H&E) staining and immunohistochemistry [MBD2 antibody (Abcam, Cambridge, United States ), MINK1 antibody (Proteintech, Wuhan, China), eosinophil antibody (anti-ECP, Biorbyt, Cambridge, United Kingdom) and neutrophil antibody (anti-Gr-1, Biolegend, San Diego, United States )]. Select stained sections per group were collected and assessed for MBD2, MINK1, eosinophil, and neutrophil protein expression.

Chen Z., Shang Y., Yuan Y., He Y., Wasti B., Duan W., Ouyang R., Jia J., Xiao B., Zhang D., Zhang X., Li J., Chen B., Liu Y., Zeng Q., Ji X., Ma L., Liu S, & Xiang X. (2022). MBD2 mediates Th17 cell differentiation by regulating MINK1 in Th17-dominant asthma. Frontiers in Genetics, 13, 959059.

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Immunological Reagents for Signaling Pathways

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Anti-TLR7 (against N-terminal for WB), anti-ATF4 and anti-Xbp1 were from Abcam. Anti-TLR7 (against C-terminal for WB) was purchased from Cell Signaling. Anti-TLR7 (against N-terminal) from Invitrogen, Anti-TLR7 (against C-terminal) and Anti-TLR7 (against C-terminal for IF) from Noves Biologicals. Anti-Pin1 and anti-ATF6 from Santa Cruz Biotechnology. SYBR Green PCR Master Mix was from Bio-Rad. R848, OVA and anti-β-actin were from Sigma. PCR primers were purchased from IDT, Inc. Protease Inhibitor Mixture was from Calbiochem. The DyLight 800/680 secondary antibodies and IMJECT Alum were purchased from Thermo Scientific. TaqMan Universial PCR Master Mix (for Rhinovirus) and TaqMan Gene Expression Assay (for SeV) were from Applied Biosystem. Sendai virus was from ATCC. Mouse TLR1–9 Agonist kit was from InvivoGen. Murine IL-5, mSCF and mFLT3L were from PeproTech. EDN (RNase2) ELISA kit, MBP (Prg2) ELISA kit and ECP ELISA kit were from MyBioSource. Anti-PRG2, anti-ECP and anti-EDN were from Biorbyt. LEGENDplx Mouse Proinflammatory Chemokine Panel was from Biolegend.

Shen Z.J., Hu J., Kashi V., Bochkov Y.A., Gern J.E, & Malter J.S. (2018). TLR-7 stress signaling in differentiating and mature eosinophils is mediated by the prolyl isomerase Pin1. Journal of immunology (Baltimore, Md. : 1950), 201(12), 3503-3513.

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Immunohistochemical Analysis of Lung Tissue

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Lung tissues were stained for immunohistochemistry (neutrophil-specific antibody (anti-Gr1, Biolegend), eosinophil antibody (anti-ECP, Biorbyt), IL-17A antibody (Proteintech), IL-4 antibody (ABBIOTEC), MBD2 antibody (Abcam), and IRF4 antibody (Proteintech)), and 2~4 H&E stained sections per group were chosen under single-blind conditions to detect and localize NEU, EOS, IL-17A, IL-4, MBD2, and IRF4 protein expression. Using a microscope and computer image processing system (Image-Pro Plus 6.0), two pathologists assessed the NEU, EOS, IL-17A, IL-4, MBD2, and IRF4 scores.

Jia A., Wang Y., Sun W., Xiao B., Wei Y., Qiu L., Mu L., Xu L., Li J., Zhang X., Liu D., Peng C., Zhang D, & Xiang X. (2017). MBD2 Regulates Th17 Cell Differentiation and Experimental Severe Asthma by Affecting IRF4 Expression. Mediators of Inflammation, 2017, 6249685.

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Histological Analysis of Lung Tissue

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Lungs were first fixed with 10% formalin via the trachea, and then removed and stored in 10% formalin. Fixed lung tissues were paraffinized and sectioned (5 μm) for hematoxylin and eosin (H&E) staining. Lung tissues were stained for immunohistochemistry (neutrophil-specific antibody [0.2 mg/ml, anti-Gr1, Biolegend, San Diego, CA, USA], eosinophil antibody [1 mg/ml, anti-ECP, Biorbyt, Cambridge, United Kingdom], MBD2 antibody [Abcam, Cambridge, United Kingdom] and HIF-1α antibody [Proteintech]). Select stained sections from each group were collected and assessed for neutrophil, eosinophil, MBD2, and HIF-1α protein expression.

Xu L., Sun W.J., Jia A.J., Qiu L.L., Xiao B., Mu L., Li J.M., Zhang X.F., Wei Y., Peng C., Zhang D.S, & Xiang X.D. (2018). MBD2 regulates differentiation and function of Th17 cells in neutrophils- dominant asthma via HIF-1α. Journal of Inflammation (London, England), 15, 15.

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