To establish the early weaning mice model, pups from the same litter were randomly divided into two groups on the basis of similar body weights (n = 3–4 per group). The suckling group lived with their mothers, whereas the weaned group lived alone. To investigate the effects of Gln supplementation on early weaning mice, early weaning pups from the same litter were randomly divided into two groups on the basis of similar body weights (n = 3–4 per group). Mice in the weaned group were free to drink water, while the Ala-Gln group was free to drink 2% L-alanyl-L-glutamine (Solarbio, Beijing, China) supplemented water, equaling a solution of 111 mM Ala-Gln. To assess the effects of alanine (Solarbio) supplementation on early weaning mice, early weaning pups from the same litter were randomly divided into two groups on the basis of similar body weights (n = 3–4 per group). The weaned group was free to drink water, while the Ala group was free to drink a solution of 111 mM of L-alanine (Solarbio) in water. Three or more weaning mice were caged together in each group. We measured the body weight every day and recorded food consumption weekly in the groups. The water was changed every 3 days. On day 7 post-weaning, the mice were sacrificed to collect jejunual tissues and blood for further analysis.
L alanine
Manufactured by Solarbio
L-alanine is an amino acid that serves as a building block for proteins. It is a white crystalline powder that is soluble in water and has a slightly sweet taste. L-alanine is commonly used in biochemical and cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Alanine, by Solarbio (1 mentions)
L proline, by Solarbio (1 mentions)
L methionine, by Solarbio (1 mentions)
Formic acid, by Merck Group (1 mentions)
Tc c18, by Agilent Technologies (1 mentions)
L valine, by Solarbio (1 mentions)
L histidine, by Solarbio (1 mentions)
L tyrosine, by Solarbio (1 mentions)
L serine, by Solarbio (1 mentions)
L phenylalanine, by Solarbio (1 mentions)
Methanol hplc grade, by Spectrum Chemical (1 mentions)
L tryptophan, by Solarbio (1 mentions)
2 protocols using l alanine
1
Early Weaning Mice Model for Glutamine Effects
2
Chiral Amino Acid Separation and Analysis
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L-Proline (L-Pro), L-methionine (L-Met), L-valine (L-Val), L-histidine (L-His), L-isoleucine (L-Ile), L-leucine (L-Leu), L-threonine (L-Thr), L-tryptophan (L-Trp), L-tyrosine (L-Tyr), L-alanine (L-Ala), L-serine (L-Ser), L-phenylalanine (L-Phe), L-arginine (L-Arg), L-asparagine (L-Asn), L-aspartic acid (L-Asp), L-cysteine (L-Cys), L-glutamine (L-Gln), L-glutamic acid (L-Glu) and L-lysine (L-Lys) are from Beijing Solarbio Science & Technology Co., Ltd. D-Proline (D-Pro), D-methionine (D-Met),D-leucine (D-Leu) and D-tryptophan (D-Trp) are from Tokyo Chemical Industry Co., Ltd. D-Guanosine is from Beijing Solarbio Since & Technology Co., Ltd. Quinine is from Saen Chemical Technology (Shanghai) Co., Ltd.
Analytical reagents methanol (HPLC grade) was purchased from Spectrum Chemical, and formic acid was purchased from Sigma Aldrich. Ultrapure water from a Heal Force water purification system (Pudong, Shanghai, China) was used to prepare solutions and the mobile phase.
The aa measurements were performed on Jasco-J1700 electronic CD at ambient temperature. The HPLC was performed on Agilent 1260 Infinity system and fitted with an Agilent TC- C18, 5 µm, 4.6 mm × 150 mm column. The column temperature was maintained at room temperature. The HPLC flow rate was 1 ml min−1 with TC-C18.
Analytical reagents methanol (HPLC grade) was purchased from Spectrum Chemical, and formic acid was purchased from Sigma Aldrich. Ultrapure water from a Heal Force water purification system (Pudong, Shanghai, China) was used to prepare solutions and the mobile phase.
The aa measurements were performed on Jasco-J1700 electronic CD at ambient temperature. The HPLC was performed on Agilent 1260 Infinity system and fitted with an Agilent TC- C18, 5 µm, 4.6 mm × 150 mm column. The column temperature was maintained at room temperature. The HPLC flow rate was 1 ml min−1 with TC-C18.
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