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Ldh cytotox non radioactive cytotoxicity assay

Manufactured by Promega
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The LDH Cytotox Non-Radioactive Cytotoxicity Assay is a colorimetric assay that measures the release of lactate dehydrogenase (LDH) from damaged cells. It provides a quantitative assessment of cell cytotoxicity or cell lysis.

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Lab products found in correlation

Proteasome glo kit, by Promega (1 mentions) Caspase glo 3 7 assay, by Promega (1 mentions)

Close spelling variants of this product

CytoTox 96 Non-Radioactive Cytotoxicity Assay (LDH; CytoTox® Non-Radioactive Cytotoxicity Assay Non-Radioactive Cytotoxicity Assay LDH cytotoxicity assay Cytotoxicity assays LDH assay

2 protocols using ldh cytotox non radioactive cytotoxicity assay

1

Cytotoxicity Assays in Neuronal and HEK Cells

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Toxicity assays in transduced primary cortical neurons (DIV7 + 14) and HEK293FT cells were performed in 96-well plates using the LDH Cytotox Non-Radioactive Cytotoxicity Assay (Promega) following the manufacturer’s instructions. To assess cell viability in HEK293FT cells, an XTT (Roche) assay was used according to the manufacturer's protocols. Cells were cultivated in a 96-well plate. Absorption was measured after 24-h incubation time. Statistical analysis was performed using GraphPad Prism (version 7.01) software.
Hartmann H., Hornburg D., Czuppa M., Bader J., Michaelsen M., Farny D., Arzberger T., Mann M., Meissner F, & Edbauer D. (2018). Proteomics and C9orf72 neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity. Life Science Alliance, 1(2), e201800070.
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2

Viability and Apoptosis Assays for Cell Lines

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Viability of HEK293FT cells and primary neurons was analyzed according to the manufacturer’s instructions in 96 well plates: LDH Cytotox Non-Radioactive cytotoxicity assay (Promega), Caspase-glo 3/7 assay (Promega), TUNEL in situ cell death detection TMR red assay (Roche). For the TUNEL assay dead and living cells were counted manually with the Fiji cell counter plugin. At least 400 cells per condition were counted per experiment in a total of three independent experiments. Proteasome activity was measured using the Proteasome-Glo kit according to the manufacturer’s instructions (Promega).
May S., Hornburg D., Schludi M.H., Arzberger T., Rentzsch K., Schwenk B.M., Grässer F.A., Mori K., Kremmer E., Banzhaf-Strathmann J., Mann M., Meissner F, & Edbauer D. (2014). C9orf72 FTLD/ALS-associated Gly-Ala dipeptide repeat proteins cause neuronal toxicity and Unc119 sequestration. Acta Neuropathologica, 128(4), 485-503.
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