Bacterial colonies were observed under a Zeiss Axioskop phase contrast microscope at a magnification of 400x. Bacterial movement was recorded using a Sony NEX-5N camera. To measure the height of colonies, S. aureus HG001(pRPO-gfp), S. aureus CGL005(pRPO-gfp) and S. aureus CGL007(pRPO-gfp) were inoculated on 25 ml TSA-0.4 plates that contained 1/100th of its volume of 0.5 μm carboxylate-modified FluoSphere beads (Invitrogen, Ex580/Em605) and cultured at 37 °C for indicated time. At each time point, one plate was moved out from the incubator, cover removed, and placed on the stage of an upright microscope for observation. Different plates were used for the indicated time point. A Z stack in which the planes were separated by 10 μm was acquired using an upright laser-scanning confocal microscope (Leica, TCS-SP2). Green fluorescence indicated the distribution of bacteria in the spreading colony. The last plane showing red fluorescence was used to indicate the plate surface. The height of the colony was determined by green fluorescence using the method described elsewhere16 (link). 3-D images of colonies were generated from the Z stacks using Imaris software.
Nex 5n camera
Manufactured by Sony
Sourced in Japan
The NEX-5N is a mirrorless interchangeable-lens camera. It features a 16.1-megapixel APS-C-size CMOS sensor, a 3-inch tilting LCD screen, and the ability to record full HD 1080p video at 60 frames per second. The camera supports interchangeable E-mount lenses.
Automatically generated - may contain errors
Lab products found in correlation
Soil moisture sensor sm150, by Delta-T Devices (2 mentions)
Axio zoom v16, by Zeiss (2 mentions)
Fluosphere beads, by Thermo Fisher Scientific (1 mentions)
Tcs sp2, by Leica (1 mentions)
Handy pea fluorimeter, by Hansatech (1 mentions)
Focus v 7, by Helicon (1 mentions)
Bx50 compound microscope, by Olympus (1 mentions)
Szx12 dissecting microscope, by Olympus (1 mentions)
E4980a lcr meter, by Agilent Technologies (1 mentions)
Dage 4000 multipurpose bondtester, by Nordson (1 mentions)
4156c semiconductor parameter analyzer, by Agilent Technologies (1 mentions)
Stereomicroscope, by Leica (1 mentions)
Murashige and skoog ms media, by Duchefa Biochemie (1 mentions)
Axioskop microscope, by Zeiss (1 mentions)
Axioskop 40, by Zeiss (1 mentions)
Calcein red, by AAT Bioquest (1 mentions)
11 protocols using nex 5n camera
1
Measuring Bacterial Colony Height Using Confocal Microscopy
2
Drought Stress Response in Transgenic Rice
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OsERF48 transgenic and NT control plants (O. sativa cv. Ilmi) were germinated on MS media at 28 °C for 3 days. Thirty plants from each line were transplanted into ten soil pots (4 × 4 × 6 cm) within a container (59 × 38.5 × 15 cm; three plants per pot) and grown for 4 weeks in a greenhouse at 28–30 °C. Pots were moved from the container for a 4‐day drought treatment and returned into the container for re‐watering until the plants recovered.
To measure chlorophyll fluorescence and the performance index, 2‐week‐old plants were transplanted into a 15‐cm‐diameter × 14‐cm‐tall pot within another larger container (66 × 45.3 × 22.5 cm) and grown for 2 months. By removing the pots from the container, drought stress was performed for 6 days. After a 1‐h dark adaptation, the longest leaves from each plant were selected and measured at their apex, middle and base regions using the Handy‐pea fluorimeter (Hansatech Instrument, Norfolk, UK). Thirty readings per line were averaged using the Handy‐pea software (version 1.31). Chlorophyll a fluorescence (Fv/Fm) and the performance index were measured and analysed according to the equations of the JIP test (Redillas et al.,2011 ). Drought‐induced symptoms were visualized using a NEX‐5N camera (Sony, Kyoto, Japan), and soil moisture was measured using a SM150 soil moisture sensor (Delta T Devices, Cambridge, UK) at the indicated time points.
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OsERF48 transgenic and NT control plants (O. sativa cv. Ilmi) were germinated on MS media at 28 °C for 3 days. Thirty plants from each line were transplanted into ten soil pots (4 × 4 × 6 cm) within a container (59 × 38.5 × 15 cm; three plants per pot) and grown for 4 weeks in a greenhouse at 28–30 °C. Pots were moved from the container for a 4‐day drought treatment and returned into the container for re‐watering until the plants recovered.
To measure chlorophyll fluorescence and the performance index, 2‐week‐old plants were transplanted into a 15‐cm‐diameter × 14‐cm‐tall pot within another larger container (66 × 45.3 × 22.5 cm) and grown for 2 months. By removing the pots from the container, drought stress was performed for 6 days. After a 1‐h dark adaptation, the longest leaves from each plant were selected and measured at their apex, middle and base regions using the Handy‐pea fluorimeter (Hansatech Instrument, Norfolk, UK). Thirty readings per line were averaged using the Handy‐pea software (version 1.31). Chlorophyll a fluorescence (Fv/Fm) and the performance index were measured and analysed according to the equations of the JIP test (Redillas et al.,
3
Standardized Protocols for Morphological Characterization
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Morphological characterisation and species descriptions were made using standardised protocols published in Samson et al. (2014) (link). Colony characters were captured on Czapek yeast autolysate agar (CYA), CYA with 5 % NaCl (CYAS), DG18, MEAbl (Oxoid LP0039 malt extract, Oxoid LP0034 peptone), MEA (Samson et al. 2010 ), oatmeal agar (OA), yeast extract sucrose agar (YES) and creatine sucrose agar (CREA). Strains were three-point inoculated on these media in 90 mm Petri dishes. Plates were incubated in darkness for 7 d at 25 °C, with additional CYA plates incubated at 30 and 37 °C. Colour names and codes used in descriptions follow Kornerup & Wanscher (1967) . Microscopic observations were made using a Zeiss AXIO Imager.M2 compound and Zeiss AXIO Zoom.V16 microscopes equipped with AxioCaM MRc5 and 512 cameras driven by Zen Blue v. 2.3 software (Carl Zeiss CMP GmbH, Göttingen, Germany). Colonies were captured with a Sony NEX-5N camera. Extended Depth of Field analysis and stacking of colony texture micrographs were performed in Helicon Focus v. 7.5.4 (HeliconSoft, Kharkiv, Ukraine). Plates were prepared in Affinity Photo v. 1.7.1 (Serif (Europe) Ltd, Nottingham, UK). For aesthetic purposes, micrographs were adjusted using the "inpainting brush tool" without altering areas of scientific significance.
4
Standardized Fungal Morphological Characterization
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Morphological characters were captured using standardized protocols proposed by Visagie et al. (2014b (link)). Colony characters were captured on Czapek yeast autolysate agar (CYA), MEAbl, yeast extract sucrose agar (YES), oatmeal agar (OA) and creatine sucrose agar (CREA). Strains were inoculated in a three-point pattern on these media in 90 mm Petri dishes. Plates were incubated for 7 d at 25 °C in darkness in perforated plastic bags. Colour names and codes used in descriptions are from Kornerup & Wanscher (1967) . Microscopic observations were made using an Olympus SZX12 dissecting microscope and Olympus BX50 compound microscope equipped with Infinity3 and InfinityX cameras driven by Infinity Analyze v. 6.5.1 software (Lumenera Corp., Ottawa, Canada). Colonies were captured with a Sony NEX-5N camera. Plates were prepared in Affinity Photo v. 1.6.6 [Serif (Europe) Ltd, Nottingham, UK]. For aesthetic purposes, micrographs were adjusted using the “inpainting brush tool” without altering areas of scientific significance. Line drawings were prepared in Affinity Photo v. 1.7.1 [Serif (Europe) Ltd, Nottingham, UK] running on an iPad Pro with an Apple Pencil.
5
Drought-Induced Stress Response in Transgenic Rice
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OsC3H10OX and OsC3H10ROX transgenic and NT control plants (Oryza sativa cv. Nakdong for OsC3H10OX; Oryza sativa cv. Ilmi for OsC3H10ROX) were germinated on MS media at 28 °C for 3 days. Thirty plants from each line were transplanted into ten soil pots (4 × 4 × 6 cm; three plants per pot) within a container (59 × 38.5 × 15 cm) and grown for two months in a greenhouse at 28–30 °C. Drought stress was imposed by removing the pots from the container for the indicated periods and rewatering for 5 days [51 (link)]. Drought-induced symptoms were visualized using a NEX-5N camera (Sony, Japan), and the soil moisture was measured using a SM150 soil moisture sensor with five repeats of each line in a random spot of each pot (Delta T Devices, UK) at the indicated time points.
6
Characterizing Paper UAV Actuator Response
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Videos of the actuator deflection response and the flight path of paper UAVs were captured using a Sony NEX-5N camera and analyzed using custom-built Matlab programs (Natick, MA, USA). The DC electrical response of the actuators and transistors and the quasi-static low-frequency capacitance were measured using an Agilent 4156C semiconductor parameter analyzer. The transistors were measured in a nitrogen atmosphere. The AC response of the actuators was measured by driving actuators using an HP 33120A function generator. The capacitance was measured using an Agilent E4980A LCR meter (Keysight, Santa Rosa, CA, USA). The mechanical stiffness of the actuators was determined by measuring the force-displacement curve under an applied tip load using a Nordson Dage 4000 Multipurpose Bondtester (Aylesbury, UK).
7
Drought Stress Assay for Arabidopsis Seedlings
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Seeds were immersed in water at 37 °C for 1 day, sowed on 1/2 MS medium containing 1% agar without sucrose and grown vertically for 1 day in the dark and 5 days under a 16 : 8 light : dark photoperiod at 28 °C. For in vitro drought conditions, polyethylene glycol (PEG)‐infused plates were prepared by dissolving solid PEG8000 in a sterilized solution of 1/2 MS medium with 6 mm 4‐morpholineethanesulfonic acid hydrate (MES) buffer (pH 5.7), followed by overlaying of the PEG solution onto 1.5% agar‐solidified half‐strength MS medium with 6 mm MES buffer (pH 5.7). The agar medium and PEG solution were equilibrated for at least 12 h, and the excess PEG solution was removed before use. Drought stress strength was considered to be proportional to the concentration of the overlaid PEG solution: 0% (control); 25% and 40% (drought stress; Verslues et al., 2006 ). To measure primary root length and lateral root number, images were taken using a NEX‐5N camera (Sony, Kyoto, Japan) and analysed with ImageJ software (https://imagej.nih.gov ). The number of lateral roots was counted within a 3‐ to 4.5‐cm region from the root tip using a stereomicroscope (Leica, Bensheim, Germany).
8
UV Microscopy of Molluscan Mantle Tissue
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Mantle tissue samples of 10 μm thickness prepared as above were fixed in 4% paraformaldehyde for 24 h, transferred to sucrose gradients, and stored in 30% sucrose. Frozen sections were stained with HE, and observed under a Nikon microscope. A number of 30 μm-thick slices of mantle tissue prepared as described above, were placed on a glass slide and dried. The sections were subjected to UV macro- or micro-photography according to a previously reported method28 (link) except for using a digital camera instead of a film camera. A UV-B emitting fluorescent tube (100 V 6 W, GL6E; Sankyo Denki, Hiratsuka, Japan) was used as a light source. A NEX-5N camera (Sony, Tokyo, Japan) without UV protection filter was used with a quartz lens (f = 25 mm, F2.8, FL-BC2528; Ricoh, Tokyo, Japan) or a Nikon Fluor 10× objective lens (NA = 0.5, 160/0.17) for photographic recording. UV band-pass filters for 320 ± 10, 337 ± 10, and 365 ± 10 nm (central wavelength ± 50% transparent band-pass wavelength: Edmund Optics Inc., Barrington, NJ, USA) were used. The camera and lens equipped with macro-extension bellows or with a macro-helicoid extension ring were set on a macrophoto-stand. The filter was inserted between the lens and the bellows or placed in front of the lens.
9
Drought Stress Response in Transgenic Rice
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Transgenic and NT control plants were germinated on Murashige and Skoog (MS) media (Duchefa, Haarlem, Netherlands) at 28 °C for 4 days, and eighteen seedlings of each transgenic lines and NT were transplanted into soil pots (4 × 4 × 6 cm; four plants per pot) and grown for 4 weeks in a greenhouse (16‐h light/8‐h dark cycle) at 28–30 °C. Each pot had the same size of holes in the bottom, and they were all placed in a single tray to synchronize watering. Drought stress was simultaneously applied to all the rice plants by first adding no water to the soil pots for 5 days and then re‐watering. Drought‐induced symptoms were monitored by imaging transgenic and NT plants at the indicated time points using a NEX‐5N camera (Sony, Tokyo, Japan).
10
Microscopic Analysis of Yeast Cell Morphology
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