Ab185986

Manufactured by Abcam

Sourced in United Kingdom

Ab185986 is a high-quality primary antibody used in immunodetection applications. It is a rabbit polyclonal antibody that targets a specific protein or antigen. The core function of this product is to specifically bind and detect the target, enabling researchers to study its expression or localization in biological samples.

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Lab products found in correlation

14 protocols using ab185986

Serum Biomarker Analysis in Jordan

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Serum assay of clinical parameters was collected into labeled Eppendorf tubes at Ibn Al-Haytham Hospital, Clinical Laboratories Department, Jordan.
The chemiluminescence immunoassay LIAISON 25OHD assay (DiaSorin, Saluggia, Italy) measured total serum 25OHD. The assay quantifies serum 25OHD and is cross-reactive with 25OHD2 and 25OHD3. Its lower limit was 4 ng/mL. An enzyme immunoassay kit measured serum leptin levels (leptin EIA-5302, DRG Diagnostics, Marburg, Germany). Test sensitivity was 0.1 ng/mL. An enzyme immunoassay kit tested serum PTH levels (PTH Intact EIA-3645, DRG Diagnostics, Marburg, Germany). The sensitivity was 1.57 pg/mL. The calcium-ARSENAZO kit (M11570i-15) and the phosphorus phosphomolybdate/Uv kit (M11508i-18, BioSystems, Barcelona, Spain) were used to measure the levels of calcium and phosphorus (PO4) in serum. Serum IL-6 concentration was measured using the Human ELISA KIT (ab178013, Abcam, Newark, NJ, USA). Using a Human ELISA KIT, serum IL-10 was measured (ab185986, Abcam). Human ELISA KIT assessed serum IL-1β (ab214025, Abcam). The Human ELISA KIT assay measured serum TNF-α (ab181421, Abcam).

Bader D.A., Abed A., Mohammad B.A., Aljaberi A., Sundookah A., Habash M., Alsayed A.R., Abusamak M., Al-Shakhshir S, & Abu-Samak M. (2023). The Effect of Weekly 50,000 IU Vitamin D3 Supplements on the Serum Levels of Selected Cytokines Involved in Cytokine Storm: A Randomized Clinical Trial in Adults with Vitamin D Deficiency. Nutrients, 15(5), 1188.

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Cytokine Quantification in HK-2 Cells

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HK-2 cells by different treatments were centrifuged at 1,000 × g for 20 min. The levels of IL-10, IL-6 and IL-1β in supernatants were examined by corresponding ELISA kits (ab185986, ab178013, ab214025, Abcam). The absorbance values at 450 nm were detected using a microplate reader (BioTek Instruments).

Zhou J., Zhang S., Sun X., Lou Y, & Yu J. (2021). Hyperoside Protects HK-2 Cells Against High Glucose-Induced Apoptosis and Inflammation via the miR-499a-5p/NRIP1 Pathway. Pathology and Oncology Research, 27, 629829.

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Cytokine Quantification via ELISA

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The cytokines such as IL-1β (BMS6002TEN), IL-2 (BMS221-2), IL-6 (BMS213-2) (Thermo Fisher Scientific Company, USA), IL-7, TNF-α (ab181421) and IL-10 (ab185986) (Abcam, UK) were scrutinized using the commercial ELISA kits following the manufacturer's instructions.

Zhang Y., Li X, & Li X. (2021). Curcumae Ameliorates Diethylnitrosamine-Induced Hepatocellular Carcinoma via Alteration of Oxidative Stress, Inflammation and Gut Microbiota. Journal of Inflammation Research, 14, 5551-5566.

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Cytokine and Growth Factor Levels in Surgical Patients

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Arterial blood samples were drawn from each patient at three time points: T1 (before the surgery), T2 (the end of the surgery), and T3 (24 hours after the surgery). The blood samples were stored in tubes containing ethylene diamine tetraacetate acid and were further processed within 12 hours. Each sample was centrifuged for 15 min at 3000rpm to obtain the plasma. Then, the levels of BMP4 (ab231930, Abcam, Cambridge, UK), Noggin (JYM2457Hu, Wuhan, CHN), IL-1β (ab217608, Abcam, Cambridge, UK), TNF-α (ab181421, Abcam, Cambridge, UK), and IL-10 (ab185986, Abcam, Cambridge, UK) were determined using the enzyme-linked immunosorbent assays method (SimpleStep ELISA^® kits, Abcam, Cambridge, UK) by a researcher who was blind to the group allocation.

Zhao X., Zhang J., Zhang W., Dai R., Xu J., Li Z, & Yang L. (2021). The Relationship Between Circulating Bone Morphogenetic Protein-4 and Inflammation Cytokines in Patients Undergoing Thoracic Surgery: A Prospective Randomized Study. Journal of Inflammation Research, 14, 4069-4077.

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Serum Cytokine and Inflammatory Marker Profiling

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The peripheral blood samples of each treatment group were collected, and the serum was collected after centrifugation, and IL-1β (Abcam Co., Ltd, Cambridge, England, ab217608), IL-2 Abcam, ab100566), IL-6 (Abcam, ab178013), IL-10 (Abcam, ab185986), IL-17 (Abcam, ab119535), IL-23 (Abcam, ab64708), tumor necrosis factor (TNF)-α (Abcam, ab181421), transforming growth receptor-β1 (Abcam, ab100647), interferon-γ(Abcam, ab174443), high-sensitivity C-reactive protein (Shuhua Biology, China, SH1297), monocyte chemotactic protein-1 (Abcam, ab179886) and intercellular cell adhesion molecule (ICAM)-1 (Abcam, ab174445) were detected by enzyme-linked immunosorbet assays. For specific steps, refer to the instruction manual of the kit (Hangzhou Lianke Biotechnology Co., Ltd, Hangzhou, China).

Yang G, & Qiu Y. (2023). Effects of amlodipine combined with atorvastatin on Th17/Treg imbalance and vascular microcirculation in hypertensive patients with atherosclerosis: A double-blind, single-center randomized controlled trial. Medicine, 102(6), e32384.

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Inflammatory Marker Quantification in GDM Placenta and Cell Culture

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ELISA kits for IL-1 (#ab235646; Abcam), IL-6 (#ab178013; Abcam), IL-8 (#ab185986; Abcam), TNF-α (#ab181421; Abcam), and CRP (#ab260058; Abcam) were used to measure the concentration of inflammatory markers in HTR-8/SVneo cell culture media of cells transfected with si-NC or targeted si-RNA and placenta homogenate of GDM patients and control. We also detected the above markers in HTR-8/SVneo cell culture media of cells treated with si-circ_0001578 and Pyrrolidinedithiocarbamate ammonium (PDTC) or SP600125. ELISA tests were used according to the manufacturer’s instructions. Briefly, 96-well plates were coated with antibodies at 4°C overnight, loaded with 100 μl of standard or condition media, and incubated at room temperature for 2 h. Samples were sequentially incubated with Detection Reagent A and B for 1 h at room temperature. Then, the antigen–antibody complex was determined by the addition of TMB substrate and observation at an OD of 450 nm. The experiments were repeated at least three times.

Zhang W., Zhao X, & Li L. (2022). Downregulationof circ_0001578 promotes gestational diabetes mellitus by inducing placental inflammation via the NF-κB and JNKs pathways. Frontiers in Endocrinology, 13, 657802.

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HNPC and Macrophage Secretome Analysis

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Supernatant from treated HNPCs and THP-1-derived macrophages were collected and centrifuged at 2,000 x g for 10 min. The supernatants then were used to ELISA according to the instruction of kits (cat. nos. CX3CL1, ab192145; IL-4, ab215089; IL-10, ab185986; CCL17, ab183366; Abcam).

Gao X.W., Hu H.L., Xie M.H., Tang C.X., Ou J, & Lu Z.H. (2023). CX3CL1/CX3CR1 axis alleviates inflammation and apoptosis in human nucleus pulpous cells via M2 macrophage polarization. Experimental and Therapeutic Medicine, 26(1), 359.

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Quantification of Inflammatory Cytokines

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HPDLSCs were lysed in 0.25% trypsin and centrifuged at 16000g for 15 min to collect the supernatant. According to the manufacturer's instructions, ELISA kits were used to detect the level of tumor necrosis factor-α (TNF-α) (ab181421, Abcam), interleukin-1β (IL-1β) (ab100562, Abcam) and interleukin-10 (IL-10) (ab185986, Abcam).

Niu F., Xu J, & Yan Y. (2022). Histone demethylase KDM5A regulates the functions of human periodontal ligament stem cells during periodontitis via the miR-495-3p/HOXC8 axis. Regenerative Therapy, 20, 95-106.

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Quantifying Inflammatory Cytokines in PBMC Co-cultures

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To measure the levels of interferon‐gamma (IFN‐γ) (ab174443), tumour necrosis factor (TNF)‐α, transforming growth factor (TGF)‐β1 (ab100647) and interleukin (IL)‐10 (ab185986) in the supernatant of co‐cultured PBMCs, commercially available enzyme‐linked immunosorbent assay (ELISA) kits from Abcam were utilized. The measurements were performed according to the instructions provided by the manufacturer.

Zhang Y., Li X., Zhang J., Mao L., Wen Z., Cao M, & Mu X. (2023). USF1 regulated circPRDM4 modulates tumorigenesis and immune escape in chemoresistant cervical cancer. Journal of Cellular and Molecular Medicine, 28(5), e17945.

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Serum Cytokine Analysis by ELISA

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The levels of midkine, IL-10, and IFN-γ in serum samples or conditional medium were evaluated by ELISA kit (Abcam # ab193761, # ab279416, # ab185986, #ab255729 and #ab174443, USA) according to manufacturer’s instructions. Briefly, samples were incubated with antibody-coated plates for 1 h at room temperature and washed for five times. Then plates were incubated with biotinylated antibody, streptavidin antibody, substrate solution, and stop solution consecutively. The absorbance at 450 nm was measured by a microplate reader. All samples were done in triplicates.

Ding L., Wang N., Wang Q., Fan X., Xin Y, & Wang S. (2023). Midkine inhibition enhances anti-PD-1 immunotherapy in sorafenib-treated hepatocellular carcinoma via preventing immunosuppressive MDSCs infiltration. Cell Death Discovery, 9, 92.

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