Envisu r2000 sd oct device

Spectral-domain optical coherence tomography (SD-OCT) was performed separately on each eye using an Envisu R2000 SD-OCT device (Bioptigen, Durham, NC). The pupils were dilated by applying 0.5% tropicamide to the cornea. The mice were anesthetized by an intraperitoneal injection of a mixture of ketamine (70 mg/kg) and xylazine (14 mg/kg), and local anesthesia was performed with a drop of oxybuprocaine (0.4% cebesine) on the cornea. Corneal hydration was maintained with Systane Ultra (Alcon, Fort Worth, TX) lubricant eye drops. Animal preparation and image acquisition were performed as previously described^{27 (link)}. Analyses were performed using a rectangular scanning protocol (1.4 mm × 1.4 mm with 1000 A-scans per B-scan × 100 B-scans) while centered on the optic nerve. The thickness of the inner nuclear layer (INL), outer nuclear layer (ONL), and photoreceptor segments (PS = IS + OS) was measured and expressed in arbitrary units using ImageJ software. Twenty measurements were performed per analysis and averaged for both eyes.

SD-OCT was performed separately on each eye of 6-month-old mice using an Envisu R2000 SD-OCT device (Bioptigen, Durham, NC). Mice were anesthetized by intraperitoneal injection of a mixture of ketamine (120 mg/kg) and xylasine (10 mg/kg) and the pupils were dilated by application of 1% tropicamide and 2.5% phenylephrine. Corneal hydration was maintained with Systane Ultra (Alcon, Fort Worth, TX) and Genteal (Novartis, Bale, Swiss) lubricant eye drops. Animal preparation and image acquisition were performed as previously described [24 (link)]. The analyses were performed using the rectangular scanning protocol (1.4 mm × 1.4 mm with 1000 A-scans per B-scan × 100 B-scans) while centered on the optic nerve. For analysis of segments, the thickness of the ONL was measured and expressed in arbitrary units. Ten measurements were performed per analysis and averaged for both eyes.