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Anti diaph2 a300 079a t

Manufactured by Fortis Life Sciences
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The Anti-DIAPH2 (A300-079A-T) is a laboratory reagent produced by Fortis Life Sciences. It is an antibody that targets the DIAPH2 protein. The core function of this product is to facilitate the detection and study of the DIAPH2 protein in experimental settings.

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Lab products found in correlation

Blebbistatin, by Cell Signaling Technology (2 mentions) Cytochalasin d, by Cayman Chemical (2 mentions) Alexa fluorophore conjugated secondary antibody, by Thermo Fisher Scientific (2 mentions) Rab5 c8b1, by Cell Signaling Technology (2 mentions) Anti fmnl3 ab57963, by Abcam (2 mentions) Anti galectin 3 ab2785, by Abcam (2 mentions) Anti human lamp 2 h4b4, by BioLegend (2 mentions) Ck869, by Cell Signaling Technology (2 mentions) Dynasore, by Cell Signaling Technology (2 mentions) Gsk269962, by Cell Signaling Technology (2 mentions) Nocodazole, by Cell Signaling Technology (2 mentions) Ml 141, by Cell Signaling Technology (2 mentions) Eht 1864, by Cell Signaling Technology (2 mentions) Bms 5, by Cell Signaling Technology (2 mentions) Smifh2, by Abcam (2 mentions) Ly294002, by Abcam (2 mentions) Rabbit anti n g polyclonal antibody, by Biosynth (2 mentions) Mouse anti cas9 7a9 3a3, by Cell Signaling Technology (1 mentions) Lc3a b d3u4c, by Cell Signaling Technology (1 mentions) Anti cas9 7a9 3a3, by Cell Signaling Technology (1 mentions)

2 protocols using anti diaph2 a300 079a t

1

Characterization of Neisseria gonorrhoeae antibodies

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The purified chicken IgY anti-Neisseria gonorrhoeae antibody was raised against formalin-killed FA1090 gonococci by Cocalico Biologicals and it cross-reacts with the MS-11, FA19 and F62 strains. The rabbit anti-N.g polyclonal antibody (cat# 20-NR08) was purchased from Fitzgerald Industries. Rabbit monoclonal antibodies against the following antigens were purchased from Cell Signaling—Rab5 (C8B1), Rab7 (D95F2), EEA1 (C45B10), GM130 (D6B1), Ezrin (#3145), LC3A/B (D3U4C), V5-Tag (E9H80), CEACAM1 (D3R80) and ACTR2 (#3128S). Mouse antibodies against the following antigens were purchased from Santa Cruz–Actin (sc8432), FMNL1 (sc390466), FMNL2 (sc390298), FHOD1 (sc365473), DAAM1 (sc100942). Mouse anti-Cas9 (7A9-3A3) was purchased from Cell Signaling. Anti-FMNL3 (ab57963) and anti-Galectin 3 (ab2785) mouse monoclonal antibodies were purchased from Abcam. Anti-human LAMP-2 (H4B4) was purchased from BioLegend. Anti-DIAPH2 (A300-079A-T) was purchased from Bethyl Labs. Highly cross-absorbed Alexa fluorophore conjugated secondary antibodies were purchased from Life Technologies.
Inhibitors used in this study were purchased from: (1) Cayman Chemicals—cytochalasin D (5μM), CK869 (10μM), Dynasore (30μM), GSK269962 (1μM), Nocodazole (3μM), ML-141 (5μM), EHT 1864 (5μM), BMS-5 (1μM) and (-) blebbistatin (5μM); (2) Cell Signaling—LY294002 (10μM); (3) Abcam—SMIFH2 (12.5μM to 100μM).
Ivanov S.S., Castore R., Juarez Rodriguez M.D., Circu M, & Dragoi A.M. (2021). Neisseria gonorrhoeae subverts formin-dependent actin polymerization to colonize human macrophages. PLoS Pathogens, 17(12), e1010184.
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2

Antibodies and Inhibitors for Gonococcal Trafficking

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The purified chicken IgY anti-Neisseria gonorrhoeae antibody was raised against formalin-killed FA1090 gonococci by Cocalico Biologicals and it cross-reacts with the MS-11, FA19 and F62 strains. The rabbit anti-N.g polyclonal antibody (cat# 20-NR08) was purchased from Fitzgerald Industries. Rabbit monoclonal antibodies against the following antigens were purchased from Cell Signaling -Rab5 (C8B1), Rab7 (D95F2), EEA1 (C45B10), GM130 (D6B1), Ezrin (#3145), LC3A/B (D3U4C), V5-Tag (E9H80), CEACAM1 (D3R80) and ACTR2 (#3128S).
Mouse antibodies against the following antigens were purchased from Santa Cruz -Actin (sc8432), FMNL1 (sc390466), FMNL2 (sc390298), FHOD1 (sc365473), DAAM1 (sc100942).
Mouse anti-Cas9 (7A9-3A3) was purchased from Cell Signaling. Anti-FMNL3 (ab57963) and anti-Galectin 3 (ab2785) mouse monoclonal antibodies were purchased from Abcam. Anti-human LAMP-2 (H4B4) was purchased from BioLegend. Anti-DIAPH2 (A300-079A-T) was purchased from Bethyl Labs. Highly cross-absorbed Alexa fluorophore conjugated secondary antibodies were purchased from Life Technologies.
Inhibitors used in this study were purchased from: (1) Cayman Chemicals -cytochalasin D (5µM), CK869 (10µM), Dynasore (30µM), GSK269962 (1µM), Nocodazole (3µM), ML-141 (5µM), EHT 1864 (5µM), BMS-5 (1µM) and (-) blebbistatin (5µM); (2) Cell Signaling -LY294002 (10µM); (3) Abcam -SMIFH2 (25µM).
Ivanov S.S., Castore R., Circu M.L., & Dragoi A. (2021). Neisseria gonorrhoeae subverts formin-dependent actin polymerization to colonize human macrophages.
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