Odyssey imager and quantification software

Protein isolation and transfer were performed as previously described (MacLeod et al., 2013 (link); Yamasaki et al., 2006 (link)). Anti-TIP39 (1 μg/ml; Santa Cruz, CA or Fitzgerald, MA), anti-PTHrP (1 mg/ml; Santa Cruz), anti-PTH2R (0.4 μg/ml; gift from Dr Usdin, NIMH), anti-glyceraldehyde-3-phosphate dehydrogenase (1 μg/ml; Fitzgerald), and antiβactin (1 μg/ml; Sigma-Aldrich, MO) antibodies were used for protein detection. IR-dye conjugated secondary antibodies (680 and 800 nm; 1:10,000) and an Odyssey imager and quantification software (LI-COR Biosciences, Lincoln, NE) were used for two-color western blotting to analyze both proteins on the same blot.

Protein isolation and transfer were performed as previously described (9 ). Anti-ATP2A2 (1μg/mL; Santa Cruz, Dallas, TX) and anti-GAPDH (1μg/mL; North Acton, MA) antibodies were used for protein detection. Six-hundred and eighty nanometer and 800 nm IR-Dye conjugated secondary antibodies (1:10000) and an Odyssey imager and quantification software (LI-COR Biosciences, Lincoln, NE) were used for two-color western blotting to analyse both proteins on the same blot.