MMCT was performed as described previously (Tomizuka et al. 1997 (link)). Briefly, the MI-MAC was transferred from CHO cells into TT2 or TT2F mES cells via mouse A9 cells by MMCT. Donor cells with the MI-MAC were treated with 0.05–0.1 µg/mL colcemid (Gibco) to form microcells. Microcells were collected by centrifugation and fused with recipient cells by polyethylene glycol (Wako, Japan). Hybrid cells containing the MI-MAC were selected in media containing 1 × HAT Supplement (Gibco) or 200 µg/mL Hygromycin B (Wako, Japan).
Polyethylene glycol
Manufactured by Fujifilm
Sourced in Japan
Polyethylene glycol is a common laboratory reagent used in various applications. It is a water-soluble, non-toxic, and chemically inert polymer that can be utilized in a range of lab procedures. The core function of polyethylene glycol is to facilitate molecular interactions, separation, and purification processes.
Automatically generated - may contain errors
Lab products found in correlation
Hygromycin b, by Fujifilm (1 mentions)
Colcemid, by Thermo Fisher Scientific (1 mentions)
Hat supplement, by Thermo Fisher Scientific (1 mentions)
Oxalic acid dihydrate, by Fujifilm (1 mentions)
Milli q integral water purification system, by Merck Group (1 mentions)
Mineral oil, by Nacalai Tesque (1 mentions)
Fluorescein isothiocyanate isomer 1 fitc, by Merck Group (1 mentions)
Thioflavin t, by Fujifilm (1 mentions)
Trifluoroacetic acid, by Fujifilm (1 mentions)
Sodium trifluoroacetate, by Fujifilm (1 mentions)
Lc ms grade acetonitrile, by Fujifilm (1 mentions)
Peg600, by Fujifilm (1 mentions)
α cyano 4 hydroxycinnamic acid, by Tokyo Chemical Industry (1 mentions)
Glycine, by Fujifilm (1 mentions)
Tannic acid, by Electron Microscopy Sciences (1 mentions)
Tween 20, by Fujifilm (1 mentions)
Glutaraldehyde, by Electron Microscopy Sciences (1 mentions)
Nabh4, by Fujifilm (1 mentions)
Osmium tetroxide, by TAAB (1 mentions)
2 hydroxy 2 methylpropiophenone hmpph, by Merck Group (1 mentions)
6 protocols using polyethylene glycol
1
Microcell-Mediated Chromosome Transfer
2
Synthesis of Metal-Organic Composite Materials
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Tin chloride dihydrate (SnCl2·2H2O), oxalic acid dihydrate, and polyethylene glycol (PEG, approximate molecular weight 400) were purchased from Fujifilm Wako Chemicals, Osaka, Japan and used as received. Ethanol (99.5%) was also purchased from Kanto Chemical Co., Inc., Tokyo, Japan. H2O (resistivity: 18.2 MΩ·cm) was obtained using a Milli-Q® integral water purification system (MERCK Ltd., Tokyo, Japan).
3
Gelatin-Lipid Hybrid Nanoparticle Fabrication
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1,2-Dioleoyl-sn-glycero-3-phosphatidylcholine
(PC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
(PE), and polyethylene glycol (molecular weight 20,000; PEG 20k) were
purchased from Wako Pure Chemical Industries (Osaka, Japan). Mineral
oil was purchased from Nacalai Tesque (Kyoto, Japan), while alkali-treated
gelatin was supplied by Nitta Gelatin Co. (Osaka, Japan). The average
molecular weight of the gelatin as determined by gel-permeation chromatography
was 190 kDa. Fluorescein isothiocyanate isomer I (FITC; Sigma-Aldrich
Japan, Tokyo, Japan) and Rhodamine B labeled 1,2-dihexadecanoyl-sn-glycero-3-phosphatidylethanolamine (Rho-PE) (Invitrogen;
Carlsbad, CA, USA) were used as fluorescent dyes for the gelatin and
lipid, respectively. Thioflavin T (ThT; Wako Pure Chemical Industries)
was used to detect β sheet rich structures like the amyloid
structures. All the materials were used without further purification.
(PC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine
(PE), and polyethylene glycol (molecular weight 20,000; PEG 20k) were
purchased from Wako Pure Chemical Industries (Osaka, Japan). Mineral
oil was purchased from Nacalai Tesque (Kyoto, Japan), while alkali-treated
gelatin was supplied by Nitta Gelatin Co. (Osaka, Japan). The average
molecular weight of the gelatin as determined by gel-permeation chromatography
was 190 kDa. Fluorescein isothiocyanate isomer I (FITC; Sigma-Aldrich
Japan, Tokyo, Japan) and Rhodamine B labeled 1,2-dihexadecanoyl-sn-glycero-3-phosphatidylethanolamine (Rho-PE) (Invitrogen;
Carlsbad, CA, USA) were used as fluorescent dyes for the gelatin and
lipid, respectively. Thioflavin T (ThT; Wako Pure Chemical Industries)
was used to detect β sheet rich structures like the amyloid
structures. All the materials were used without further purification.
4
Sensitive MALDI-TOF MS Analysis
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BPA, trifluoroacetic acid (TFA), LC/MS grade acetonitrile (ACN), polyethylene glycol (PEG) 200 and PEG600, sodium trifluoroacetate (TFANa) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). 2,5-Dihydroxybenzoic acid (DHB) and α-Cyano-4-hydroxycinnamic acid (CHCA) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan).
5
Cytoskeletal Structure Analysis
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Glutaraldehyde and tannic acid were purchased from Electron Microscopy Sciences (Hatfield, PA); osmium tetroxide from TAAB (Berks, England); and glycine, polyethylene glycol, Tween 20, and NaBH4 from Wako (Osaka, Japan).
6
Cellulose-Based Hydrogel Synthesis
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Cotton-derived fibrous cellulose was obtained by cutting and milling of Whatman filter paper No. 5 (GE Healthcare Life Sciences Corp.). Sulfuric acid (Nacalai Tesque, Inc.) and polyethylene glycol (PEG; molecular weight = ~20,000, Wako Pure Chemical Ind., Ltd.) were used as received. Monomers, HEMA (Wako Pure Chemical Ind., Ltd.) and AA (Nacalai Tesque, Inc.), were purified by vacuum distillation before use. A photopolymerization initiator, 2-hydroxy-2-methylpropiophenone (HMPPh; Sigma-Aldrich), and a cross-linking agent, ethylene dimethacrylate (EDM; Tokyo Kasei Kogyo Co., Ltd.), were used without further purification.
Calcium chloride dihydrate (CaCl 2 •2H 2 O) and ammonium carbonate ((NH 4 ) 2 CO 3 ) were purchased from Nacalai Tesque, Inc. and used as ion sources for mineralization. A low-molecular-weight PAA (L-PAA; average degree of polymerization (DP) = 25, Sigma-Aldrich) was used as a precipitation inhibitor in the salt solution of ion server [25, 37] . Other conventional chemicals were purchased from Wako Pure Chemical Ind., Ltd. or Nacalai Tesque, Inc. and used as received.
Calcium chloride dihydrate (CaCl 2 •2H 2 O) and ammonium carbonate ((NH 4 ) 2 CO 3 ) were purchased from Nacalai Tesque, Inc. and used as ion sources for mineralization. A low-molecular-weight PAA (L-PAA; average degree of polymerization (DP) = 25, Sigma-Aldrich) was used as a precipitation inhibitor in the salt solution of ion server [25, 37] . Other conventional chemicals were purchased from Wako Pure Chemical Ind., Ltd. or Nacalai Tesque, Inc. and used as received.
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