Mpo elisa kit

The levels of cf-DNA and MPO-DNA complexes were measured using MPO ELISA kit (Finetest, China) and dsDNA Assay Kit (Invitrogen, USA) as previously described. Mouse pancreas was freshly collected, fixed with 10% neutral buffered formalin and embedded in paraffin. Pancreatic tissues were sectioned into 3 μm. After antigen repair with citric acid-containing antigen repair buffer under high pressure, pancreatic tissue sections were blocked with 5% (wt/vol) BSA and incubated with the primary antibody Histone H3 (citrulline R2+R8+R17, ab5103, 1:1000, Abcam, USA) and Myeloperoxidase (GB12224, 1:1000, Servicebio, China) overnight at 4°C. After three washes, the sections were subsequently incubated with Alexa Fluor 568/647 conjugated secondary antibodies (1:1000, Invitrogen, USA) for 1 h at room temperature and counterstained with DAPI (1:2000, Sigma, Sweden).

The effect of L-NAT pretreatment on myeloperoxidase activity in irradiated Neuro2a cells was evaluated using the myeloperoxidase (MPO) Elisa Kit (EM0010, Fine Test). As previously stated, Neuro2a cells were divided into four experimental groups. According to the manufacturer protocol provided with the kit, and MPO activities were assessed at different times in each group (i.e., 2, 4, 24, and 48 h). The absorbance was measured at 450 nm by a spectrofluorometer (Synergy BIO-TEK Instrument USA).