Fresh olive leaves were collected from olive trees (Olea europaea L.) grown at the Faculty of Agriculture, Kagawa University, Japan. Breast fillet used in this study were obtained from 43-day old ROSS 308 broiler chickens which were given feed mixtures dedicated for broiler chickens and were stored at 4°C after slaughter. Sodium polyphosphate was obtained from Kirin Kyowa Foods Co. Ltd. (Tokyo, Japan). Thiobarbituric acid (TBA) malondialdehyde standard was purchased from Cayman Chemical(Michigan, USA). Folin-Ciocalteu reagent was purchased from Nacalai Tesque Inc (Kyoto, Japan). All other chemicals used were of analytical grade.
Folin ciocalteu reagent
Manufactured by Nacalai Tesque
Sourced in Japan
The Folin-Ciocalteu reagent is a chemical solution used in colorimetric assays to determine the total phenolic content in various samples. It is a mixture of phosphomolybdic and phosphotungstic acids that react with phenolic compounds, producing a blue-colored complex that can be measured spectrophotometrically.
Automatically generated - may contain errors
Lab products found in correlation
2 2 azobis 2 amidinopropane dihydrochloride, by Fujifilm (2 mentions)
Trifluoroacetic acid, by Fujifilm (1 mentions)
Pyrogallol, by Fujifilm (1 mentions)
Methanol, by Fujifilm (1 mentions)
Acetonitrile, by Fujifilm (1 mentions)
Fluorescein, by Nacalai Tesque (1 mentions)
Methyl β cyclodextrin, by Fujifilm (1 mentions)
Methyl tert butyl ether (mtbe), by Fujifilm (1 mentions)
β carotene, by Fujifilm (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchromane 2 carboxylic acid, by Merck Group (1 mentions)
β cryptoxanthin, by Funakoshi (1 mentions)
Sodium carbonate, by Nacalai Tesque (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Fujifilm (1 mentions)
Fluorescein sodium salt, by Merck Group (1 mentions)
P hydroxybenzaldehyde, by Fujifilm (1 mentions)
Gallic acid monohydrate, by Fujifilm (1 mentions)
P coumaric acid, by Tokyo Chemical Industry (1 mentions)
1 1 diphenyl 2 picrylhydrazyl dpph, by Merck Group (1 mentions)
3 protocols using folin ciocalteu reagent
1
Olive Leaf and Broiler Chicken Fillet
2
Antioxidant Evaluation and Characterization
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The following reagents were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan): 2,2′-azobis(2-amidinopropane) dihydrochloride (AAPH), acetonitrile, methanol, methyl tert-butyl ether (MTBE), methyl-β-cyclodextrin, β-carotene, trifluoroacetic acid (TFA), and pyrogallol. Fluorescein and Folin-Ciocalteu reagent were purchased from Nacalai Tesque, Inc. (Kyoto, Japan). Additionally, 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) was purchased from Sigma-Aldrich Co. (St. Louis, MO, USA), and β-cryptoxanthin was purchased from Funakoshi Co., Ltd. (Tokyo, Japan).
3
Antioxidant Potential of Sugarcane Compounds
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The following reagents were from commercial sources. Gallic acid monohydrate, 2,2 -azobis (2-amidinopropane) dihydrochloride (AAPH) , 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox) , 2,2 -azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) , and phydroxybenzaldehyde were purchased from Wako Pure Chemical Industries (Osaka, Japan) . Folin-Ciocalteu reagent, sodium carbonate, and p-hydroxyacetophenone were purchased from Nacalai Tesque (Kyoto, Japan) . Fluorescein sodium salt and 1,1-diphenyl-2-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich (St. Louis, MO., U.S.A.) . p-Coumaric acid was purchased from Tokyo Chemical Industries (Tokyo, Japan) . Schaftoside and isoschaftoside were isolated from sugarcane products and identified based on NMR and UV-Vis spectra previously 8) . All other reagents were from Wako Pure Chemical Industries.
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