Genomic DNA was isolated with GenElute Mammalian genomic DNA isolation kit (catalog no.: G1N70-1KT; Sigma), and PCR was carried out with GoTaq G2 Hot Start Green Master Mix (catalog no.: M7422; Promega). PCR product bands were detected following separation on a 1% agarose gel and isolated by cutting out the gel band and isolation with Monarch DNA gel extraction kit (catalog no.: T1020S; NEB).
Total RNA was isolated with Miniprep RNeasy Mini Kit (catalog no.: 74104; Qiagen), and first strand complementary DNA (cDNA) synthesis was carried out with Qscript cDNA synthesis Supermix (catalog no.: 95048-100; Quanta Biosciences). For the quantitative PCR, we used SensiFast SYBR Hi-ROX kit (catalog no.: BIO92005; Labgene), and the reaction was performed on StepOnePlus Real-Time PCR System (catalog no.: 4376600; Thermo Fisher Scientific). The following primers were used: 18s forward 5′-GGCCCTGTAATTGGAATGAGTC-3′, 18s reverse 5′-CCAAGATCCAACTACGAGCTT-3’; UNC93B1 forward 5′-CTGCTCACCTTCATCCTCTTT-3′, UNC93B1 reverse 5′-GTGCTGAGTCCAGTCTTGTT-3’; STIM1 forward 5′-CCTCTCTTGACTCGCCATAATC-3′, STIM1 reverse 5′-CTTGGAGTAACGGTTCTGGATATAG-3’.
Total RNA was isolated with Miniprep RNeasy Mini Kit (catalog no.: 74104; Qiagen), and first strand complementary DNA (cDNA) synthesis was carried out with Qscript cDNA synthesis Supermix (catalog no.: 95048-100; Quanta Biosciences). For the quantitative PCR, we used SensiFast SYBR Hi-ROX kit (catalog no.: BIO92005; Labgene), and the reaction was performed on StepOnePlus Real-Time PCR System (catalog no.: 4376600; Thermo Fisher Scientific). The following primers were used: 18s forward 5′-GGCCCTGTAATTGGAATGAGTC-3′, 18s reverse 5′-CCAAGATCCAACTACGAGCTT-3’; UNC93B1 forward 5′-CTGCTCACCTTCATCCTCTTT-3′, UNC93B1 reverse 5′-GTGCTGAGTCCAGTCTTGTT-3’; STIM1 forward 5′-CCTCTCTTGACTCGCCATAATC-3′, STIM1 reverse 5′-CTTGGAGTAACGGTTCTGGATATAG-3’.