Synergy purification system

Manufactured by Merck Group

Sourced in United States

The Synergy purification system is a laboratory equipment designed for the purification of various biomolecules. It utilizes advanced chromatographic techniques to separate and purify proteins, nucleic acids, and other macromolecules from complex mixtures. The system is capable of performing a wide range of purification tasks with high efficiency and reproducibility.

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Lab products found in correlation

Chromabond c18 ec, by Macherey-Nagel (1 mentions) 2 4 6 8 tetramethyl 2 4 6 8 tetravinylcyclotetrasiloxane, by Merck Group (1 mentions) Su 8 2007, by MicroChem (1 mentions) Platinum wire, by Thermo Fisher Scientific (1 mentions) 3 3 dioctadecyloxacarbocyanine perchlorate dio, by Thermo Fisher Scientific (1 mentions) Gold seal coverslips, by Electron Microscopy Sciences (1 mentions) Acetone, by Avantor (1 mentions) Isopropanol, by Avantor (1 mentions) Bovine insulin, by Merck Group (1 mentions) Mcf 7 cell, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by American Type Culture Collection (1 mentions) Eagle s minimum essential medium emem, by American Type Culture Collection (1 mentions) Anti bcl 2 antibody, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Su 8 2075 photoresist, by MicroChem (1 mentions) Angiotensin 2, by Bruker (1 mentions) Angiotensin 1, by Bruker (1 mentions) Bombesin, by Bruker (1 mentions) Acth clip 1 17, by Bruker (1 mentions)

4 protocols using synergy purification system

Xanthylium Cation Extraction Protocol

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SPE was carried out using multimode cartridges CHROMABond C18 ec (Macherey-Nagel GmbH & Co. KG, Düren, Germany) consisting of an octadecyl-modified silica gel with a bed volume of 10 g. The method is based on the extraction procedure of George et al. [23 (link)]. The cartridges were conditioned with 120 mL ethanol and then equilibrated with 120 mL ultrapure water obtained from a Synergy purification system (Millipore, Molsheim, France). The sample (30 mL) was loaded onto the cartridges. The xanthylium cations were collected during this step. The cartridges were then washed with 120 mL of water, followed sequentially by 120 mL of 5% and 7.5% aqueous ethanol solutions, and 70 mL of 25% aqueous ethanol solution. The ethyl esters of the xanthylium cations were then eluted with 50 mL of 40% ethanol. The solvents were evaporated after SPE and the residue was subsequently lyophilized.

Bührle F., Gohl A, & Weber F. (2017). Impact of Xanthylium Derivatives on the Color of White Wine. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 22(8), 1376.

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Fabrication of PDMS Microfluidics

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Si wafers (4 in) were obtained from University Wafer. The negative photoresist SU-8 2007 and developer were purchased from Microchem, USA. (Tridecafluoro-1,1,2,2-tetrahydrooctyl)dimethylchlorosilane (TDTS) for wafer silanization was purchased from Gelest Corp., USA. Sylgard184, composed of the silicon elastomer base and the curing agent for poly(dimethylsiloxane) (PDMS) was obtained from Dow Corning Corporation, USA. For h-PDMS, vinyl PDMS prepolymer, Pt calalyst (platinum divinyltetramethyldisiloxane), and hydrosilane prepolymer were purchased from Gelest Corp, USA and a modulator (2,4,6,8-Tetramethyl-2,4,6,8-tetravinylcyclotetrasiloxane) from Sigma-Aldrich, USA. Deionized water was supplied from a Synergy purification system (Millipore, USA).

Nakano A., Camacho-Alanis F, & Ros A. (2015). Insulator-Based Dielectrophoresis with β-Galactosidase in Nanostructured Devices. The Analyst, 140(3), 860-868.

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Polystyrene Bead Characterization and Lipid Vesicle Formation

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0.28- (FP-0262–2) and 0.87-μm-diameter (FP-0852–2) polystyrene beads exhibiting negative surface charges were purchased from Spherotech (Lake Forest, IL, USA). SYLGARD^® 184 silicone elastomer kit for polydimethylsiloxane (PDMS) was purchased from Dow Corning Corporation (Midland, MI, USA). 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES), poly(ethylene glycol)-block-poly(propylene glycol)-block-poly(ethylene glycol) (brand name Pluronic^® F108), potassium hydroxide (KOH), potassium chloride (KCl), potassium phosphate dibasic anhydrous (K₂HPO₄), magnesium chloride (MgCl₂), dimethyl sulfoxide (DMSO), Phosphatidylcholine (PC), phosphatidylethanolamine (PE), phosphatidylserine (PS), cholesterol and sucrose were purchased from Sigma-Aldrich (St. Louis, MO, USA). Deionized (DI) water was obtained from a Synergy purification system (Millipore, USA). Fisherbrand^® Plain Microscope glass slides (75 × 50 × 1.0 mm; USA), DiD (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine Perchlorate) and DiO (3,3’-Dioctadecyloxacarbocyanine Perchlorate) were purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Gold-Seal coverslips were purchased from Electron Microscopy Sciences (48 × 60 mm, No. 1; Hatfield, PA, USA). Platinum wire was purchased from Alfa Aesar (Ward Hill, MA, USA). Extruder kits were purchased from Avanti Polar Lipids (Alabaster, AL).

Kim D., Luo J., Arriaga E, & Ros A. (2018). A Deterministic Ratchet for Sub-micrometer (Bio)particle Separation. Analytical chemistry, 90(7), 4370-4379.

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Quantitative Proteomic Analysis of MCF-7 Cells

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MCF-7 cells, eagle’s minimum essential medium (EMEM), and fetal bovine serum were obtained from ATCC (USA). Dulbecco’s phosphate-buffered saline (DPBS) buffer and the Hausser scientific hemocytometer were purchased from Thermo Fisher Scientific (USA). Acetonitrile, alpha-cyano-4-hydroxycinnamic acid (α-CHCA), human Bcl-2, anti-Bcl-2 antibody, bovine serum albumin (BSA), and bovine insulin were purchased from Sigma-Aldrich (USA). The peptide calibration mixture (angiotensin II 1046.5418 m/z [M+H]⁺, angiotensin I 1296.6848 m/z [M+H]⁺, substance P [1347.7354 m/z [M+H]⁺, bombesin 1619.8223 m/z [M+H]⁺, ACTH clip 1-17 2093.0862 m/z [M+H]⁺, ACTH clip 18-39 2465.1983 m/z [M+H]⁺, and somtostatin-28 3147.4710 m/z [M+H]⁺) was purchased from Bruker Daltonics Inc (USA). Isotope labeled peptides (FATVVEEL(d10)FR and FATVVEEL(13C15N)FR) were purchased from GenScript USA Inc. Polydimethylsiloxane (PDMS) was purchased from Dow Corning Corporation (USA). Indium tin oxide (ITO) coated glass slides (100 ohm/sq) were obtained from NANOCS (USA). Cr was obtained from SPI Supplies (USA). Tridecafluoro-1, 1, 2, 2-tetra-trichlorosilane (TTTS) was purchased from Gelest (USA). Isopropanol and acetone were from VWR (USA). SU-8 2075 photoresist and SU-8 developer were from MicroChem (USA). Ultrapure water was supplied from a synergy purification system (Millipore, USA).

Yang M., Villarreal J.C., Ariyasinghe N., Kruithoff R., Ros R, & Ros A. (2021). A quantitative approach for protein analysis in small cell ensembles by an integrated microfluidic chip with MALDI mass spectrometry. Analytical chemistry, 93(15), 6053-6061.

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