1H NMR spectra measurements were examined in d6-DMSO and CDCl3 at 25°C using Bruker AVANCE ΙΙΙ 400 (Madison, WI, USA) operating at 400 MHz. The number average molecular weight (Mn) and polydispersity index (Mw/Mn) were determined by gel permeation chromatography (GPC) adopting an Agilent 1200 series GPC system (Santa Clara, CA, USA) equipped with a LC quant pump, PL gel 5 mm 500, 104, and 105 Å columns in series, and RI detector. The column system was calibrated with a set of monodisperse polystyrene standards using HPLC grade THF as mobile phase with a flow rate of 1.0 mL/min at 30°C. Fluorescence spectra were recorded using a fluorescence spectrophotometer (F-4500, Hitachi, Chiyoda-ku, Japan). The hydrodynamic diameter (Dh) and distribution (PDI) of micelles were measured by dynamic light scattering (DLS, Malvern Zetasizer Nano S, Malvern, WR, UK). Morphologies of micelles were investigated by transmission electron microscopy (TEM, Hitachi H-7650) operating at 80 kV.
Agilent 1200 series gpc system
Manufactured by Agilent Technologies
Sourced in United States, Switzerland
The Agilent 1200 series GPC system is a high-performance gel permeation chromatography (GPC) system used for the analysis of macromolecular compounds such as polymers and proteins. The system provides accurate and reliable results for the determination of molecular weight and molecular weight distribution of these materials.
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2 protocols using agilent 1200 series gpc system
1
Micelle Characterization Protocol
2
Comprehensive Polymer Characterization Protocol
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Gel permeation chromatography (GPC) was executed by an Agilent 1200 series GPC system (liquid chromatogram quant pump, polar gel 5 mm 500 Å, 10,000 Å, and 100,000 Å columns in series, refractive index detector, mobile phase: high-performance liquid chromatography grade tetrahydrofuran, 1.0 mL/min, 30°C) to determine the average molecular weight (Mn) and polydispersity index (Mw/Mn).
Proton nuclear magnetic resonance (1H NMR) test was adopted on a spectrometer (AVANCE III 400, Bruker, 250 MHz, Switzerland) at 25°C. Deuterated chloroform (CDCl3-d) and tetramethylsilane were used as solvent and an internal standard, respectively.
Dynamic light scattering (Malvern Zetasizer Nano S, Malvern, UK) was carried out to detect the particle size (Dh) and distribution (polydispersity index). Briefly, after being purified by a 0.45 μm pore size filter, the received samples were measured in a 1.0 mL quartz cuvette using a diode laser of 800 nm at 25°C. The scattering angle was 90°.
Fourier transform infrared (FT-IR) spectrophotometer (Nicolet Nexus for Euro, USA) was used to confirm the structure of the synthesized polymer. The spectra were taken from 400 cm−1 to 4,000 cm−1.
Transmission electron microscopy (TEM, Hitachi H-7650, Japan) was used to detect the morphologies of polymeric micelles and DOX-loaded particles operating at 80 kV.
Proton nuclear magnetic resonance (1H NMR) test was adopted on a spectrometer (AVANCE III 400, Bruker, 250 MHz, Switzerland) at 25°C. Deuterated chloroform (CDCl3-d) and tetramethylsilane were used as solvent and an internal standard, respectively.
Dynamic light scattering (Malvern Zetasizer Nano S, Malvern, UK) was carried out to detect the particle size (Dh) and distribution (polydispersity index). Briefly, after being purified by a 0.45 μm pore size filter, the received samples were measured in a 1.0 mL quartz cuvette using a diode laser of 800 nm at 25°C. The scattering angle was 90°.
Fourier transform infrared (FT-IR) spectrophotometer (Nicolet Nexus for Euro, USA) was used to confirm the structure of the synthesized polymer. The spectra were taken from 400 cm−1 to 4,000 cm−1.
Transmission electron microscopy (TEM, Hitachi H-7650, Japan) was used to detect the morphologies of polymeric micelles and DOX-loaded particles operating at 80 kV.
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