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Esi l low concentration tuning mix g1969 85000

Manufactured by Agilent Technologies
Sourced in United States

The ESI-L low concentration tuning mix (G1969-85000) is a calibration solution designed for use with Agilent's electrospray ionization (ESI) mass spectrometry instruments. It provides a range of low-concentration reference compounds to help optimize and calibrate the performance of the mass spectrometer.

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4 protocols using esi l low concentration tuning mix g1969 85000

1

Characterization of Biological Compounds

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Purified water was obtained by using a Milli-Q Integral 3 system (Millipore SAS, Molsheim, France). Zomepirac sodium salt (used as internal standard, IS), L-pyroglutamic acid, hypoxanthine, L-histidine monohydrochloride monohydrate, oleic acid, L(+) lactic acid, LS-MS grade acetonitrile, methanol, formic acid, and LC grade ethanol were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low-concentration tuning mix (G1969-85000), and ESI-TOF Biopolymer Analysis reference masses (G1969-850003) were purchased from Agilent Technologies (Santa Clara, CA, USA).
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2

Mass Spectrometry Reagent Standards

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Purified water was obtained using the Milli-Qplus185 system (Millipore, Billerica, MA, USA). LC–MS grade methanol (MeOH), acetonitrile (ACN), formic acid and LC grade ethanol (EtOH), standard mix for GC–MS, containing grain fatty acid methyl ester mixture (C8-C28), 2-propanol and analytical grade heptane were purchased from Sigma-Aldrich ChemieGmbH (Steinheim, Germany). C18:0methyl ester and N,O-bis(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane were obtained from Pierce Chemical Co (Rockford, IL, USA). Silylation grade pyridine was purchased from VWR International BHDProlabo (Madrid, Spain). The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low concentration tuning mix (G1969-85000) and ESI-TOF Biopolymer Analysis referencemasses (G1969-850003) were purchased from Agilent Technologies (Santa Clara, CA, USA).
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3

Preparation of LC-MS Grade Solvents

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Purified water was obtained using the Milli-Q Integral 3 system (Millipore SAS, Molsheim, France). LC-MS grade methanol, acetonitrile, formic acid and LC grade acetone, and ethanol were purchased from Sigma-Aldrich Chemie GmbH, Steinheim, Germany. The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low concentration tuning mix (G1969-85000), and ESI-TOF Biopolymer Analysis reference masses (G1969-850003) were purchased from Agilent Technologies, Santa Clara, California, USA.
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4

Mass Spectrometry Protocol Optimization

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Purified water was obtained using the Milli-Q Integral 3 system (Millipore SAS, Molsheim, France). Zomepirac sodium salt (used as internal standard, IS), LS-MS-grade acetonitrile (ACN), methanol (MeOH), and formic acid (FA) were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany). Pure p.a. ammonium solution (25%) was purchased from Avantor Performance Materials (Gliwice, Poland). The API-TOF reference mass solution kit (G1969-850001) and tuning solutions, ESI-L low concentration tuning mix (G1969-85000), and ESI-TOF Biopolymer Analysis reference masses (G1969-850003) were purchased from Agilent Technologies (Santa Clara, CA, USA). Standards to confirm the identity of metabolites were purchased from Sigma-Aldrich Chemie GmbH (Steinheim, Germany) (taurine, pipecolic acid, leucine, histidine, phenylalanine, 3-methylhistidine, citric acid, tryptophan, adenosine, and stearoylcarnitine) and Avanti Polar Lipids Inc. (AL, USA) (LPI 18:0 and LPG 18:1).
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