BON cells treated with dacarbazine (0–600µM) ± ABT-888 (0-10µM) were collected and incubated with PE Annexin V and 7-Aminoactinomycin D (7-AAD) fluorescein solutions (BD Pharmingen, San Jose, CA, USA) according to the manufacturer’s protocol. The FACSCalibur™ (BD Biosciences, San Jose, CA, USA) fluorescent-activated cell-sorting (FACS) instrument was used for quantitative fluorescent sorting, and FlowJo v10.0.8 (TreeStar Inc., Ashland, OR, USA) was used for subsequent analysis.
7 aminoactinomycin d 7 aad fluorescein
Manufactured by BD
Sourced in United States
7-Aminoactinomycin D (7-AAD) fluorescein is a fluorescent dye used for DNA staining in flow cytometry applications. It binds to DNA and emits fluorescence upon excitation, allowing for the detection and analysis of cellular DNA content.
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Lab products found in correlation
2 protocols using 7 aminoactinomycin d 7 aad fluorescein
1
Assessing Apoptosis in BON Cells
2
Assessing Apoptosis in BON Cells
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