Immunohistochemistry was performed on all cases, either at the time of original diagnosis or for the purposes this study. Whole-slide sections of formalin-fixed, paraffin embedded tumor tissue were cut at five-micron thickness, deparaffinized, and subjected to antigen retrieval using 10 mM citrate buffer at 92°C for 30 minutes. Immunohistochemistry was performed on all cases using antibodies for S100 (Ventana Medical Systems, Tucson, AZ), either Smooth Muscle Actin (Ventana) or Calponin (Dako, Carpinteria, CA), SOX10 (BioCare Medical, Concord, CA), Desmin (Dako), and β-catenin (BD Biosciences, San Jose, CA). In addition, for a subset of cases (n=6), PAX3 (Bioss Inc., Wolburn, MA) immunohistochemistry was also performed. Depending on tissue availability, immunohistochemistry was also performed in most cases using antibodies for myogenin (Ventana) and Factor XIIIa (Cell Marque, Rocklin, CA). All immunohistochemical signals were visualized using the Ultra view polymer detection kit (Ventana Medical Systems, Inc. Tucson, AZ) on a Ventana BenchmarkXT autostainer (Ventana). Staining was performed according to manufacturer's instructions in the presence of appropriate controls. “Focal” immunoreactivity was regarded as immunostaining in <10% of tumor cells.
Factor xiiia
Manufactured by Cell Marque
Factor XIIIa is a laboratory reagent used in diagnostic testing. It is a critical component in the blood coagulation cascade, playing a key role in the stabilization of fibrin clots. This product is designed for use in in-vitro diagnostic procedures.
Automatically generated - may contain errors
Lab products found in correlation
Desmin, by Agilent Technologies (2 mentions)
Myogenin, by Roche (1 mentions)
Sox10, by Biocare Medical (1 mentions)
Ultra view polymer detection kit, by Roche (1 mentions)
Calponin, by Agilent Technologies (1 mentions)
Ventana benchmark xt, by Roche (1 mentions)
β catenin, by BD (1 mentions)
Desmin, by Roche (1 mentions)
Ventana benchmark ultra slide stainer, by Roche (1 mentions)
Stat6, by Roche (1 mentions)
Cd163, by Cell Marque (1 mentions)
Cd163, by Roche (1 mentions)
2 protocols using factor xiiia
1
Immunohistochemical Profiling of Rare Tumors
2
Immunohistochemical Analysis of Skin Tumor
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The excision specimen from the left foot comprising skin and subcutaneous tissue was received in buffered neutral formalin. The tumor in the specimen was extensively sampled and processed for routine hematoxylin and eosin–stained sections. Five-μm sections from formalin-fixed, paraffin-embedded tissues were stained with antibodies against CD31 (Neomarker), CD34 (DAKO), ERG (Cell Marque), STAT6 (Cell Marque), Factor XIIIa (Cell Marque), CD163 (Cell Marque), desmin (DAKO), and SMA (DAKO) using the Ventana Optiview detection kit on the Roche Ventana BenchMark ULTRA slide stainer (Roche Diagnostics) after antigen retrieval, if required. For CD34, STAT6, Factor XIIIa, CD163, and desmin stains, the ULTRA cell conditioning method on the Roche Ventana BenchMark ULTRA slide stainer was used for antigen retrieval. For ERG and CD31 stains, the pressure cooker with citrate buffer at pH6 method was employed. For SMA, no antigen retrieval treatment was required.
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