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Methyl farnesoate

Manufactured by Echelon Biosciences
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Sourced in United States

Methyl farnesoate is a chemical compound that serves as a core laboratory product for research purposes. It is an important intermediate in the biosynthesis of various hormones and other biologically active molecules. The compound can be used as a reference standard or as a building block in the synthesis of related compounds for scientific investigations.

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Lab products found in correlation

20 hydroxyecdysone 20e, by Merck Group (1 mentions) Farnesoic acid, by Echelon Biosciences (1 mentions) Methoprene, by Merck Group (1 mentions) Pyriproxyfen, by Merck Group (1 mentions) Restriction enzymes, by Fujifilm (1 mentions) O nitrophenyl β d galactopyranoside onpg, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by Nacalai Tesque (1 mentions) Dithiothreitol (dtt), by Nacalai Tesque (1 mentions) Pyruvate, by Merck Group (1 mentions) Mevalonic acid 5 phosphate, by Merck Group (1 mentions) Acetyl coa, by Merck Group (1 mentions) Mevalonic acid, by Merck Group (1 mentions)

6 protocols using methyl farnesoate

1

Solvent Preparation for Bioassays

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Methyl farnesoate (Echelon Biosciences Inc., Salt Lake City, Utah), and all other chemicals (Sigma-Aldrich Corp., St. Louis, MO) screened in BRET and luciferase reporter gene assays, were dissolved in DMSO for delivery to assay solutions. Final DMSO concentrations were 0.001% v/v in the BRET assays and 0.0005% v/v in the reporter gene assays. Hydroprene and diofenolan were dissolved in ethanol for in vivo experiments, where the final concentration of ethanol was 0.0003% v/v.
Medlock Kakaley E.K., Eytcheson S.A, & LeBlanc G.A. (2017). Ligand-Mediated Receptor Assembly As An Endpoint For High-Throughput Chemical Toxicity Screening. Environmental science & technology, 51(16), 9327-9333.
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2

Genetically Identical Male and Female Production

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To produce genetically identical males and females, standardized mothers were exposed to a short pulse of the juvenile hormone, methyl farnesoate (Product ID: S‐0153, Echelon Biosciences), following previously established protocols (Thompson et al., 2017). Briefly, mothers were exposed to the hormone at a concentration of 300 μg/L after producing their first clutch and then transferred into fresh hormone‐treated media every 2 days. This treatment encourages the production of male offspring with identical genotypes to their maternal generation (Olmstead & Leblanc, 2002), with no confounding fitness effects on the lifespan, fecundity, infection rates, or spore loads of the offspring (Thompson et al., 2017). All experimental animals were taken from clutches three and four of the hormone‐treated mothers.
Laidlaw T., Hector T.E., Sgrò C.M, & Hall M.D. (2020). Pathogen exposure reduces sexual dimorphism in a host’s upper thermal limits. Ecology and Evolution, 10(23), 12851-12859.
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3

Preparation of Steroid Hormone Solutions

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20-hydroxyecdysone (20E) was provided by Sigma (St. Louis, MO). Stock of aqueous solutions of 20E were prepared at a concentration of 10−5 M and stored in aliquots at −80 °C. Farnesoic acid (FA) and methyl farnesoate (MF) were purchased from Echelon Biosciences (Salt Lake city, UT).
Areiza M., Nouzova M., Rivera-Perez C, & Noriega F.G. (2015). 20-hydroxyecdysone stimulation of juvenile hormone biosynthesis by the mosquito corpora allata. Insect biochemistry and molecular biology, 64, 100-105.
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4

Insect Hormone Synthesis and Assays

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Dimethyl sulfoxide (DMSO) and dithiothreitol (DTT) were obtained from Nacalai Tesque (Kyoto, Japan). JH III, (S)‐hydroprene, methoprene, pyriproxyfen, and o‐nitrophenyl‐β‐D‐galactopyranoside (ONPG) were purchased from Sigma‐Aldrich Chemical Co. (St. Louis, MO, USA). Methyl farnesoate was obtained from Echelon Biosciences Inc. (Salt Lake City, UT, USA). Fenoxycarb was supplied from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). JH 0, I, and II were synthesized as previously described [46 (link)]. Restriction enzymes, DNA modification enzymes, and other chemicals were obtained from Wako Pure Chemical Industries, Ltd. (Osaka Japan), Nacalai Tesque, Sigma‐Aldrich Chemical Co., TaKaRa Bio Inc. (Otsu, Japan), or TOYOBO Co., Ltd. (Osaka, Japan).
Ito‐Harashima S., Matsuura M., Takada E., Kawanishi M., Nakagawa Y, & Yagi T. (2021). Detection of juvenile hormone agonists by a new reporter gene assay using yeast expressing Drosophila methoprene‐tolerant. FEBS Open Bio, 11(10), 2774-2783.
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5

Methyl Farnesoate Assay Protocol

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Methyl farnesoate (Echelon Biosciences Inc., Salt Lake City, Utah), was dissolved in DMSO for delivery to the assay solutions. Final DMSO concentration in all assay solutions including controls was 0.001% v/v in BRET assays and 0.0005% v/v in luciferase reporter gene assays.
Kakaley E.K., Wang H.Y, & LeBlanc G.A. (2017). Agonist-mediated assembly of the crustacean methyl farnesoate receptor. Scientific Reports, 7, 45071.
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6

Aea Peptide Synthesis and Characterization

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Custom made peptide Aedes aegypti AST-C (AeaAST-C) (QIRYRQCYFNPISCF) was provided by Biopetide Co. (San Diego, CA), purified by reverse phase liquid chromatography and assessed to be 98% pure by analytical mass spectrometry and amino acid analysis. Stock of aqueous solutions of AeaAST-C were prepared at a concentration of 10−5 M and stored in aliquots at −80 °C. Acetate, citrate, pyruvate, acetyl-CoA (ACoA), mevalonic acid (MVA), mevalonic acid 5-phosphate (MevP), mevalonic acid 5-pyrophosphate (MevPP), farnesal (FAL) and 1,2,3-benzenetricarboxylate (BTC) were purchased from Sigma (St. Louis, MO). Farnesyl diphosphate (FPP), farnesol (FOL), farnesoic acid (FA) and methyl farnesoate (MF) were purchased from Echelon Biosciences (Salt Lake city, UT).
Nouzova M., Rivera-Perez C, & Noriega F.G. (2014). Allatostatin-C reversibly blocks the transport of citrate out of the mitochondria and inhibits juvenile hormone synthesis in mosquitoes. Insect biochemistry and molecular biology, 57, 20-26.
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