Reagents for this study included: TRIzol reagent (Thermo Fisher Scientific, USA), citrate buffer (pH=6.0) (Wuhan Boshide Corporation, China), qSYBR Green PCR kit (Shanghai GenePharma, Shanghai, China), reverse reaction kit (Promega, USA), 10×RT buffer (Dalian Bao Biotech Company, China), DEPC water (Sigma-Aldrich, USA), MMLV reverse transcriptase (Dalian Bao Biotech Company, China), 2.5 mM dNTP mixture (Nanjing Kaiji Biotech., China), 10× PCR buffer (Promega, USA), fetal bovine serum (Thermo Fisher Scientific, USA), trypsin/EDTA (Thermo Fisher Scientific, USA), Transwell chamber (Merck, USA), Lipofectamine TM 2000 (Thermo Fisher Scientific, USA), CCK-8 (Tongren Chemical Research Institute, Japan), Annexin V-FITC Apoptosis Detection Kit (Promega, USA), and RPMI-1640 medium (Life Technologies, Gaithersburg, MD).
Annexin 5 fitc apoptosis detection kit
Manufactured by Promega
Sourced in Japan
The Annexin V-FITC Apoptosis Detection Kit is a laboratory product that enables the detection and analysis of apoptosis in cells. Annexin V, a protein that binds to phosphatidylserine, is labeled with the fluorescent dye FITC and used as a marker for the early stages of apoptosis. The kit provides the necessary reagents and buffers to perform this assay.
Automatically generated - may contain errors
Lab products found in correlation
Trypsin edta, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Reverse reaction kit, by Promega (1 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions)
Depc water, by Merck Group (1 mentions)
Transwell chamber, by Merck Group (1 mentions)
Qsybr green pcr kit, by GenePharma (1 mentions)
Pcr buffer, by Promega (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
In cell analyzer 2200, by GE Healthcare (1 mentions)
3 protocols using annexin 5 fitc apoptosis detection kit
1
Transcriptional Regulation of Cell Proliferation
2
Cell Cycle and Apoptosis Analysis
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Cell cycle analysis was performed using a cell cycle assay kit (Promega) according to the manufacturer's instructions. Flow cytometry cell cycle analysis was performed using a FACSAria I flow cytometer. The ModFit LT software was used to quantify DNA ploidy. Apoptosis was quantified using an Annexin V–FITC Apoptosis Detection Kit (Promega) according to the manufacturer's instructions. The data were analyzed using the FlowJo software (version 7.6.1; TreeStar). The cell surface differentiation antigen CD11b was detected by FACS. Briefly, 1 × 106 cells were stained with FITC-anti-human CD11b for 20 min at room temperature. The cells were then washed and resuspended in FACS buffer. The cells were analyzed immediately after immunostaining using the FACSAria I and FlowJo Version 7.6.1 software.
3
Quantifying Cell Viability and Apoptosis
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Following incubation of cells with the indicated treatments, propidium iodide (10 μg/ml) and Hoechst (1 μg/ml) were added 30 min before imaging. Cells were imaged on an IN Cell Analyzer 2200 (GE Healthcare; 10 × objective). Live/dead cell analysis was performed using IN Cell analysis software.
Apoptosis was assessed using Annexin PI staining as per Promega Annexin V-FITC apoptosis detection kit instructions.
Apoptosis was assessed using Annexin PI staining as per Promega Annexin V-FITC apoptosis detection kit instructions.
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