Total RNA of cells (106) was extracted with Trizol and was reverse transcribed with PrimeScriptTM RT Maseter Mix (Takara, RR036A). Real-time PCR was performed with SYBR Premix Ex TaqTM IIz (Takara, RR820A), using a ROCHE LightCycler® 480. The samples were analyzed using the 2-△△Ct method from the Ct values of the respective RNAs (PKG, cGMP, and CYP11B2) relative to the housekeeping gene GAPDH. The following primers were used. cGMP primer (sense: 5′-ATTGGGATTGTGGGCCATGT-3′; antisense: 5′-TTGTCACGTAGTCGGTGAGC-3′); PKG primer (sense: 5′-CGTTACCCGAGAAGACTCACC-3′; antisense: 5′-GAGACATCATCCAGTCCTCCAA-3′); CYP11B2 primer (sense: 5′-TGCTAAATGGGGCTGACTGG-3′; antisense: 5′-CATCCATGGTAAGGCTCCCTC-3′).
Sybr premix ex taqtm iiz
Manufactured by Takara Bio
SYBR Premix Ex TaqTM IIz is a ready-to-use PCR master mix that contains SYBR Green I dye, Taq DNA polymerase, dNTPs, and buffer components. It is designed for real-time quantitative PCR applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using sybr premix ex taqtm iiz
1
Real-time PCR Analysis of Signaling Pathways
2
Real-time PCR Analysis of sGC, cGMP, and PKG
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA of cells (106) was extracted with Trizol and was reverse transcribed with PrimeScriptTM RT Maseter Mix (Takara, RR036A). Real-time PCR was performed with SYBR Premix Ex TaqTM IIz (Takara, RR820A), using a ROCHE LightCycler® 480. The samples were analyzed using the 2−△△Ct method from the Ct values of the respective RNAs (sGC, cGMP, and PKG) relative to the housekeeping gene GAPDH. The following primers were used. sGC primer (sense: 5′- ACGGAGCCAATAGAGGAGGT-3′; antisense: 5′- TGCCAGGGTGTGTAGGTAGA-3′); cGMP primer (sense: 5′-ATTGGGATTGTGGGCCATGT-3′; antisense: 5′-TTGTCACGTAGTCGGTGAGC-3′); PKG primer (sense: 5′-CGTTACCCGAGAAGACTCACC-3′; antisense: 5′-GAGACATCATCCAGTCCTCCAA-3′).
3
Analyzing Progerin and Oxidative Stress Markers in Blood Granulocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA of the peripheral blood granulocytes of patients and rat primary LSECs (106) was extracted with Trizol and reverse-transcribed with PrimeScriptTM RT Maseter Mix (Takara, #RR036A). Real-time PCR was performed with SYBR Premix Ex TaqTM IIz (Takara, #RR820A), using ROCHE LightCycler®480. The samples were analyzed using the 2-∆∆Ct method from the Ct values of the respective RNAs (progerin, NOX2, NOX4, LMNA, Lamin B1, and Cav-1) relative to the housekeeping gene GAPDH. The primer sequences are displayed in the Supplementary Materials . The experiment was repeated more than three times.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!