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Coomassie blue dye

Manufactured by Cayman Chemical
Sourced in United States

Coomassie blue dye is a synthetic dye commonly used in biochemistry and molecular biology laboratories. It is a highly sensitive and versatile dye that can be used to detect and quantify proteins in a variety of applications, such as protein electrophoresis and western blotting.

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2 protocols using coomassie blue dye

1

Combination Therapy for Breast Cancer

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The oils of Borage and Medium Chain Triglyceride (MCT) were obtained from the electronic store iHerp.com. Tween 80 and Span 20 were purchased from Al Shafei medical and Scientific Equipment, Est (Jeddah, KSA). Docetaxel (DTX) was kindly provided by King Abdulaziz University Hospital. Thymoquinone (TQ) and Sulforhodamine B (SRB) dye were obtained from Sigma-Aldrich (USA). The Coomassie blue dye was purchased from Cayman Chemical (Michigan, US). Annexin V-FITC apoptosis detection kit was obtained from Invitrogen Abcam (UK). Acridine Orange and rapamycin were obtained from BDH Chemicals Ltd (England, UK) and Enzo Life Sciences (Lausen, Switzerland), respectively. Human Breast cancer cell lines, MCF-7 and MDA-MB-231, were obtained from ATCC (American Type Tissue Culture Collection, Manassas, VA, USA).
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2

Evaluating Anticancer Potential of Natural Compounds

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For cell culture, Dulbecco's modified eagle medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin, and trypsin-EDTA were all obtained from Gibco Life Technologies (Grand Island, NY, USA). TQ was purchased from Sigma-Chemical Co (St. Louis, MO, USA). DTX was kindly supplied for this study by King Abdulaziz University Hospital. The sulforhodamine B (SRB) dye was purchased from Biotium Inc. (Hayward, CA, USA). Pure Borage seed oil was acquired from Seychelles organics, Inc. (FL, USA), and MCT oil was obtained from Nature's Way Brands LLC Green Bay (WI, USA). The surfactants of Tween 80 and Span 20 were acquired from Sigma–Aldrich, Inc. (Saint Louis, MO, USA). Coomassie blue dye and propidium iodide (PI) were purchased from Cayman Chemical (Ann Arbor, MI, USA). Human breast cancer cell T47D was procured from the America Type Tissue Culture Collection (ATCC, Manassas, VA, USA).
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