Transmission electron microscopy of cells was performed as previously described (22 (link)). RCC cells were treated with ROC-325 for 24 h and harvested for imaging. Briefly, sections were cut in an LKB Ultracut microtome (Leica), stained with uranyl acetate and lead citrate, and examined in a JEM 1230 transmission electron microscope (JEOL, USA, Inc.). Images were captured using the AMT Imaging System (Advanced Microscopy Techniques Corp).
Lkb ultracut microtome
Manufactured by Leica
Sourced in United States
The LKB Ultracut microtome is a precision instrument designed for sectioning biological samples for microscopic analysis. It provides accurate and consistent slice thicknesses ranging from 0.5 to 2,000 microns, allowing for the preparation of high-quality specimens for a variety of applications.
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3 protocols using lkb ultracut microtome
1
Transmission Electron Microscopy of RCC Cells
2
Reovirus Infection Quantification by EM
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Cells were treated with 30 PFU/cell Reolysin for 48 h and processed for electron microscopy. Sections were cut in an LKB Ultracut microtome (Leica, Deerfield, IL), stained with uranyl acetate and lead citrate, and examined in a JEM 1230 transmission electron microscope (JEOL, USA, Inc., Peabody, MA). The percentage of reovirus positive cells were determined by counting 500 cells in random fields using an electron microscope. The fraction of cytoplasm containing containing reoviral particles was quantified using ImageJ software.
3
Reolysin Treatment of MM Cells
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MM cells and PBMCs were treated with 30 PFU/Cell Reolysin for 48 h and processed for electron microscopy. Sections were cut in an LKB Ultracut microtome (Leica, Deerfield, IL), stained with uranyl acetate and lead citrate, and examined in a JEM 1230 transmission electron microscope (JEOL, USA, Inc., Peabody, MA).
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