Precision herg cho recombinant cell line cyl3038

All testing was carried out
in CHO cells transfected with the hERG gene, purchased from Millipore
(PrecisION hERG-CHO Recombinant Cell Line CYL3038). The cell line
was grown in DMEM/F-12, GlutaMAX with 10% fetal bovine serum, 1% penicillin–streptomycin,
1% geneticin, and 1% of 1 M HEPES buffer solution, and maintained
at approximately 37 °C in a humidified atmosphere containing
5% carbon dioxide. The cells were passaged every 3–5 days based
on confluency. On the day of the experiment, 50%–80% confluent
cells were harvested from a 175 cm² culture flask using
Detachin. After 10 min of exposure to Detachin at 37 °C, the
cells were centrifuged for 1 min at 1000 rpm. The supernatant was
removed, and the cell pellet was reconstituted in 5–8 mL of
serum-free media with 2.5% of 1 M HEPES, placed on the Qstirrer, and
allowed to recover. After a ∼30 min recovery period, experiments
were initiated.

All testing was carried out
in CHO cells transfected with the hERG gene, purchased from Millipore
(PrecisION hERG-CHO Recombinant Cell Line CYL3038). The cell line
was grown in DMEM/F-12, GlutaMAX with 10% fetal bovine serum, 1% penicillin–streptomycin,
1% geneticin, and 1% of 1 M HEPES buffer solution and maintained at
approximately 37 °C in a humidified atmosphere containing 5%
carbon dioxide. The cells were passaged every 3–5 days based
on confluency. On the day of the experiment, 50%–80% confluent
cells were harvested from a 175 cm² culture flask using
Detachin. After 10 min of exposure to Detachin at 37 °C, the
cells were centrifuged for 1 min at 1000 rpm. The supernatant was
removed, and the cell pellet was reconstituted in 5–8 mL of
serum-free media with 2.5% of 1 M HEPES, placed on the Qstirrer, and
allowed to recover. After a ∼30 min recovery period, experiments
were initiated.