Bio plex pro mouse diabetes panel

Manufactured by Bio-Rad

Sourced in United States

The Bio-Plex Pro Mouse Diabetes Panel is a multiplex assay that allows for the simultaneous detection and quantification of multiple analytes related to diabetes in mouse samples. The panel measures a range of biomarkers, providing a comprehensive assessment of the diabetes-related factors in a single experiment.

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Spelling variants of this product

Bio-Plex (286 citations) Bio‐Plex diabetes panel (2 citations) Bio-Plex Pro™ Mouse Diabetes Panel 8-Plex (2 citations)

6 protocols using bio plex pro mouse diabetes panel

Quantification of Mouse Serum Metabolites

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Serum triglycerides were quantified using a Triglyceride Colorimetric Assay Kit (Cayman Chemicals, Ann Arbor, MI). Metabolically active hormones were quantified using the Bio-Plex Pro Mouse Diabetes Panel (Bio-Rad Laboratories, Hercules, CA). Both were prepared according to the manufacturer’s instructions. Freshly obtained blood samples were allowed to clot for 30 min at room temperature before undergoing centrifugation at 5000 rcf for 5 min to obtain serum samples. We utilized an 8-plex Bio-Plex Pro Mouse Diabetes Panel from Bio-Rad (cat. #171F7001M, Hercules, CA) to quantify ghrelin, GIP, GLP-1, glucagon, insulin, leptin, PAI-1, and resistin in mouse serum.

Mims T.S., Abdallah Q.A., Stewart J.D., Watts S.P., White C.T., Rousselle T.V., Gosain A., Bajwa A., Han J.C., Willis K.A, & Pierre J.F. (2021). The gut mycobiome of healthy mice is shaped by the environment and correlates with metabolic outcomes in response to diet. Communications Biology, 4, 281.

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Plasma Insulin Resistance Markers

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Mice were injected I.P. with 10 mg/ml ketamine/xylazine. Blood was collected from the portal vein using an insulin syringe and collected into ETDA-coated microfuge tubes. Blood was centrifuged at 5000 rpm for 10 minutes at 4°C and plasma supernatant was stored at −80°C. Insulin resistance markers and gut peptide hormones were measured using Bio-Rad’s Bio-Plex Pro™ Mouse Diabetes Panel (Hercules, CA) following manufacturer’s instructions.

Martinez-Guryn K., Hubert N., Frazier K., Urlass S., Musch M.W., Ojeda P., Pierre J.F., Miyoshi J., Sontag T., Cham C., Reardon C., Leone V, & Chang E.B. (2018). Small intestine microbiota regulate host digestive and absorptive adaptive responses to dietary lipids. Cell host & microbe, 23(4), 458-469.e5.

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Serum Protein and Hormone Analysis

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Concentrations of several proteins were measured in serum collected at euthanasia. All proteins measured are listed in Table S2. All hormones were measured via a BioPlex Pro Mouse Diabetes Panel (BioRad, Hercules, CA), except insulin-like growth factor (IGF)-1 and vascular endothelial growth factor (VEGF), which were assessed with BioRad singleplex assays. Cytokines were measured via a BioPlex Pro Mouse Cytokine Panel. All assays were read on a BioRad MAGPIX multiplex analyzer.

Bowers L.W., Glenny E.M., Punjala A., Lanman N.A., Goldbaum A., Himbert C., Montgomery S.A., Yang P., Roper J., Ulrich C.M., Dannenberg A.J., Coleman M.F, & Hursting S.D. (2022). Weight loss and/or sulindac mitigate obesity-associated transcriptome, microbiome, and protumor effects in a murine model of colon cancer. Cancer prevention research (Philadelphia, Pa.), 15(8), 481-495.

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Serum Metabolic Hormone and Cytokine Analysis

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One week before tumor injection, blood was collected from all mice by submandibular bleed after fasting 4–6 hours, and serum was processed and stored. In 5–8 mice/group, serum concentrations of a panel of metabolic hormones and growth factors was measured using a BioPlex Pro Mouse Diabetes Panel (BioRad, Hercules CA). Adiponectin, insulin-like growth factor (IGF)-1 and transforming growth factor beta 1 (TGF-β1) were measured with BioRad singleplex assays, while a panel of cytokines was measured using a BioPLex Pro Mouse Cytokine Panel (Bio-Rad). A BioRad MAGPIX multiplex analyzer was used to read all assays, as previously described (17 (link)).

Bowers L.W., Doerstling S.S., Shamsunder M.G., Lineberger C.G., Rossi E.L., Montgomery S.A., Coleman M.F., Gong W., Parker J.S., Howell A., Harvie M, & Hursting S.D. (2022). Reversing the genomic, epigenetic and triple negative breast cancer-enhancing effects of obesity. Cancer prevention research (Philadelphia, Pa.), 15(9), 581-594.

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Serum Biomarker Quantification Protocol

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Serum insulin and leptin concentrations were measured using the Bio-Plex Pro mouse diabetes panel (BioRad, Hercules, CA, USA). Serum IGF1 concentrations were measured using the IGF1 Mouse Magnetic Luminex Screening Assay (R&D Systems, Minneapolis, MN, USA). Assays were conducted according to manufacturer’s protocols on a Bio-Plex MAGPIX system (BioRad).

Bustamante-Marin X., Devlin K.L., McDonell S.B., Dave O., Merlino J.L., Grindstaff E.J., Ho A.N., Rezeli E.T., Coleman M.F, & Hursting S.D. (2023). Regulation of IGF1R by MicroRNA-15b Contributes to the Anticancer Effects of Calorie Restriction in a Murine C3-TAg Model of Triple-Negative Breast Cancer. Cancers, 15(17), 4320.

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Quantifying Serum Triglycerides and Metabolic Hormones

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Mims T.S., Abdallah Q.A., Stewart J.D., Watts S., White C.T., Rousselle T., Bajwa A., Han J.C., Willis K.A., & Pierre J.F. (2020). The gut mycobiome of healthy mice is shaped by the environment and shapes metabolic outcomes in response to diet.

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