The largest database of trusted experimental protocols

6 protocols using myristic acid

1

Fatty Acid and Antioxidant Dietary Effects

Check if the same lab product or an alternative is used in the 5 most similar protocols
Flies were reared on either a plain diet (Formula 4–24 Plain Drosophila Medium; Carolina Biological Supply); a diet with the addition of one of the following FAs at 5 μL/mL unless otherwise indicated: linoleic acid (L1012; Sigma-Aldrich), stearic acid (198-12481; Wako), or myristic acid (134-03435; Wako); or a diet with 25 IU/mL α-tocopherol (207-01792; Wako) as reported previously (24 (link), 77 (link), 78 (link)).
+ Open protocol
+ Expand
2

Antioxidant Activity Evaluation Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Shikonin and alkannin were purchased from Wako Pure Chem. Ind. Ltd. (Osaka, Japan) and stocked frozen (−20°C) in small aliquots as 30 mM solutions in dimethylsulfoxide (DMSO; Wako). Myristic acid, sodium salt (MA), 2,2'-azobis(2-aminopropane) dihydrochloride (AAPH), riboflavin, Trolox, sodium diethyldithiocarbamate (DETC), and ferrous sulfate were also from Wako. Cytochrome c (type VI from horse heart), β-NADPH, superoxide dismutase (SOD), and oxyhemoglobin (Oxy-Hb) were from Sigma. Spin trapping reagent 5-(2,2-dimethyl-1,3-propoxy cyclophosphoryl)-5-methyl-1-pyrroline-N-oxide (CYPMPO) was from Radical Research (Tokyo, Japan). 3-(2-hydroxy-1-methyl-2-nitrosohydrazino)-N-methyl-1-propanamine (NOC-7), EDTA2Na, and n-heptyl-β-thioglucoside were from Dojindo Laboratories (Kumamoto, Japan). All the reactions were performed under aerobic conditions, and the solutions were not deaerated.
+ Open protocol
+ Expand
3

Fatty Acid and Lipid Standards

Check if the same lab product or an alternative is used in the 5 most similar protocols
Caprylic acid (C8:0), capric acid (C10:0), lauric acid (C12:0), myristic acid (C14:0), stearic acid (C18:0), arachidonic acid (C20:4n6), and methyl-d3 stearate standard were obtained from Wako Pure Chemicals (Tokyo, Japan).
+ Open protocol
+ Expand
4

Generating Fatty Acid-Bound BSA

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lauric acid (12:0, Nacalai Tesque, Kyoto, Japan), myristic acid (Wako Pure Chemicals), palmitic acid (16:0, Wako Pure Chemicals) and oleic acid (18:1, Nacalai Tesque) were dissolved in DMSO. To generate bovine serum albumin‐bound fatty acids, each fatty acid was mixed with an equal volume of phosphate‐buffered saline containing 10% (w/v) fatty acid‐free bovine serum albumin (Wako Pure Chemicals). After sonication, the mixture was added to cell culture medium (0.5% v/v final concentration of DMSO), and the cells were further incubated for 24–48 h. The cultured cells were harvested for western blot analysis.
+ Open protocol
+ Expand
5

Organic Acid Intercalation into LDH Nanohybrids

Check if the same lab product or an alternative is used in the 5 most similar protocols
The organic monocarboxylic acids sodium salts; n-capric acid (CH3(CH2)8COOH), myristic acid (CH3(CH2)12COOH), and stearic acid (CH3(CH2)16COOH) were obtained from WAKO Company (Tokyo, Japan). The supplies of dicarboxylic acids sodium salts (suberic acid COOH(CH2)6COOH and sebacic acid COOH(CH2)8COOH) were from TCI Company (Tokyo, Japan). The nanohybrids were formed through anions exchange reactions. Typically, the appropriate concentration of organic acid (0.2 M) was prepared through dissolving 0.002 mol of the sodium salt of aliphatic acids in 10 mL of deionized–distilled water. By using ultrasonic technique, this aqueous solution of organic acid was mixed with 0.5 g of the prepared Co/Zn/Ti LDH for performing intercalation reactions. This process was achieved under an argon atmosphere with strong stirring at room temperature for 24 h. The product was filtrated and washed many times by deionized–distilled water. The fine powder was obtained after drying under vacuum.
+ Open protocol
+ Expand
6

Synthesis and Characterization of Myristic Acid Derivatives

Check if the same lab product or an alternative is used in the 5 most similar protocols
Myristic acid (purity: 98%) and glycerol (CH 2 OH-CHOH-CH 2 OH, purity: 99.8%) used in this investigation were obtained from Emery Oleochemicals, Singapore. Potassium hydroxide (purity: 85.0%) was purchased from Wako, Tokyo, and was used for the neutralization of Myristic acid. Cosmetic grade of 1,3-butylene glycol (CH 2 OH-CH 2 -CHOH-CH 3 , Daicel, Osaka) , di-propylene glycol (CH 3 -CHOH-CH 2 -O-CH 2 -CHOH-CH 3 , Asahi Glass, Tokyo) , and polyethylene glycol 400 (HO-(CH 2 -CH 2 -O) n -H, PEG400, Toho Chemicals, Tokyo) were used without further purification. Water was deionized using an Organo FW-10 system.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!