Serum HBV DNA level was quantified by TaqMan 48 automatic fluorescence quantitative polymerase chain reaction (q-PCR) kits using a Roche COBAS AmpliPrep/COBAS TaqMan 48 Analyzer (Roche Diagnostics, Mannheim, Germany); the detection limit was 12 IU/ml [9 (link)].
Cobas ampliprep cobas taqman 48 analyzer
Manufactured by Roche
Sourced in United States
The COBAS Ampliprep/COBAS TaqMan 48 Analyzer is a fully automated system designed for nucleic acid extraction and real-time PCR amplification and detection. It is capable of performing integrated sample preparation and real-time PCR analysis for a variety of diagnostic applications.
Automatically generated - may contain errors
Lab products found in correlation
8 protocols using cobas ampliprep cobas taqman 48 analyzer
1
Quantification of Serum HBV DNA
2
HBV Infection Serum Biomarker Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The serum samples of 328 untreated HBeAg-positive Chinese CHB patients with GTB or GTC HBV infection were from our previous registered clinical study (NCT01088009) [21 (link), 22 (link)]. The informed consents were obtained. The clinical diagnosis of CHB was performed according to the Chinese Society of Hepatology. Patients with hepatic decompensation or hepatocellular carcinoma were excluded according some criteria described in our previous study [22 (link)]. The serum samples were negative for hepatitis C virus, hepatitis D virus and human immunodeficiency virus serum markers and stored in aliquots at -80 ℃ until use.
The serum HBsAg level was detected by ARCHITECT HBsAg kit (Abbott Diagnostics, Chicago, United States) [22 (link)]. Serum level of HBV DNA was quantitated by TaqMan 48 automatic fluorescence quantitative PCR kits using Roche COBAS AmpliPrep/COBAS TaqMan 48 Analyzer (Roche Diagnostics, Mannheim, Germany) [22 (link)]. Serum level of HBV RNA was quantitated by our published in-house assay [21 (link)].
The serum HBsAg level was detected by ARCHITECT HBsAg kit (Abbott Diagnostics, Chicago, United States) [22 (link)]. Serum level of HBV DNA was quantitated by TaqMan 48 automatic fluorescence quantitative PCR kits using Roche COBAS AmpliPrep/COBAS TaqMan 48 Analyzer (Roche Diagnostics, Mannheim, Germany) [22 (link)]. Serum level of HBV RNA was quantitated by our published in-house assay [21 (link)].
3
Measuring HIV-1 RNA and CD4+ Counts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The FACSCalibur (BD, Franklin Lakes, New Jersey, USA) flow cytometer were used to measured absolute blood CD4+ T-cell counts (cells/μL). The levels of plasma HIV-1 RNA (copies/mL) were detected by the COBAS Ampliprep/COBAS TaqMan 48 Analyzer (Roche Diagnostics, Branchburg, New Jersey, USA).
4
Quantitation of HIV-1 RNA in Plasma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
COBAS® AmpliPrep/COBAS® TaqMan 48 Analyzer (Roche Molecular Systems, USA), a nucleic acid amplification test system, were used for the detection and quantitation of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in human plasma. Automated COBAS AmpliPrep Instrument was used for specimen preparation whereas COBAS TaqMan 48 Analyzer was used for amplification and detection, following the manufacturer’s instruction. The limit of detection was <20 copies/Ml.
5
Serum Biomarker Assessment Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum ALT, aspartate aminotransferase (AST), total bilirubin (TB), creatine kinase (CK), serum creatinine (SCr), and blood urea nitrogen (BUN) were measured using an Olympus Au5400 automatic biochemistry analyzer (Olympus, Tokyo, Japan). HBV DNA was detected using a COBAS AmpliPrep/COBAS TaqMan 48 analyzer (Roche Diagnostics, Basel, Switzerland).
6
HBV DNA Quantification Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HBV DNA quantitative detection was assessed by COBAS AmpliPrep/ COBAS TaqMan 48 Analyzer (Roche Molecular Systems, Branchburg, NJ, the USA) with the HBV nucleic acid quantification detection kit according to the manufacturer’s instructions (Roche Molecular Systems).
7
Standardized HCV Diagnostic Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A standardized sample collection and data analysis protocol was applied at the five liver centers, including ELISA for serum anti-HCV antibodies (Roche Molecular Diagnostics, NJ, United States), highly sensitive, real-time PCR-based assay for HCV RNAs (LOD 15 IU/mL; COBAS Ampliprep/COBAS TaqMan 48 Analyzer, Roche), direct sequencing of serum or intrahepatic RNA for HCV genotyping (SinoMD, China), and Fibroscan for liver fibrosis (Echosens, France). Serum samples negative in routine HCV RNA tests were further ultracentrifuged and retested. If the ultracentrifuged serum remained HCV negative, PBMC were tested. In OCI patients, HCV RNA levels were re-assessed every 12 wk after initiation of antiviral treatment until the end of treatment or follow up. CHC patients had serum HCV RNA tests at 4 and 12 wk after initiation of treatment, and then every 12 wk until the end of treatment or follow up.
8
Comprehensive HBV Serological and Molecular Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
HBV serologic markers, including HBsAg, anti-HBs antibody, hepatitis B e antigen (HBeAg), anti-HBe and anti-HBc antibodies titers were assayed with a chemi-luminescent microparticle immunoassay using an automated Abbott AxSYM analyzer (Abbott, USA). HBV DNA levels were measured by real-time polymerase chain reaction (PCR) assay using a COBAS AmpliPrep/COBAS TaqMan 48 analyzer (Roche Diagnostics, Switzerland).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!