Perisoft for windows version 2

Laser Doppler measurements were made at a sample rate of 33 samples per second, and averaged over 1-min intervals using Perisoft for Windows, version 2.5.5 (Perimed AB, J€ arf€ alla, Sweden). The mean perfusion and the RBC concentration were measured with LSCI and PLSI, respectively, in five regions of both the irradiated and the non-irradiated breast: the upper inner quadrant (UIQ), lower inner quadrant (LIQ), upper outer quadrant (UOQ), lower outer quadrant (LOQ), and areola (A) (Figure 1). The mean perfusion in these regions was calculated by selecting regions of interest in the perfusion images using the LSCI system's software (PimSoft 1.3, Perimed AB, J€ arfalla, Sweden). Mean PLSI indices (RBC concentrations) were calculated from PLSI image files in the same manner as above using ImageJ (version 1.49t, US National Institutes of Health, Bethesda, MD). The inner quadrants and areola were measured from the frontal images, while the outer quadrants were measured from the lateral images.