Eticlopride hydrochloride

Manufactured by Bio-Techne

Sourced in United Kingdom

Eticlopride hydrochloride is a selective dopamine D2 receptor antagonist. It is a white to off-white crystalline powder used in research applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Eticlopride (6 citations)

5 protocols using eticlopride hydrochloride

Nicotine, D2 Receptor Modulation in Neurons

Check if the same lab product or an alternative is used in the 5 most similar protocols

Quinpirole hydrochloride, eticlopride hydrochloride, and gabazine (SR 95531 hydrobromide) were purchased from Tocris Bioscience. (-)-Nicotine hydrogen tartrate salt was purchased from Sigma-Aldrich. 15 min after the first nicotine injection, quinpirole 1 mg/kg was administered intravenously. 5 minutes later, eticlopride 1 mg/kg was administered intravenously. When the neuron activity was returned to baseline, a second nicotine injection was performed. Once D2-R pharmacology was applied, no further neurons were recorded and the animal was discarded.

Eddine R., Valverde S., Tolu S., Dautan D., Hay A., Morel C., Cui Y., Lambolez B., Venance L., Marti F, & Faure P. (2015). A concurrent excitation and inhibition of dopaminergic subpopulations in response to nicotine. Scientific Reports, 5, 8184.

+ Open protocol

+ Expand

Electrochemical Measurements in Artificial CSF

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals were purchased from EMD Chemicals Inc. (Darmstadt, Germany) unless otherwise stated. Dopamine hydrochloride, Tris base, Tris hydrochloride, 3,4-ethylenedioxythiophene (EDOT), and GBR-12909 were purchased from Sigma-Aldrich (St. Louis, MO). Eticlopride hydrochloride was purchased from Tocris Bioscience (Avonmouth, Bristol, U.K.). Nafion solution (LQ-1105) was purchased from Ion Power Inc. (New Castle, DE) and used as provided. Ketamine was purchased from Phoenix Pharmaceuticals Inc. (Ketaject, St. Joseph, MO). Artificial cerebral spinal fluid (aCSF) was made by dissolving 15 mM Tris, 126 mM NaCl, 2.5 mM KCl, 20 mM NaHCO₃, 2.0 mM NaH₂PO₄, 1.2 mM Na₂SO₄, 1.2 mM CaCl₂, and 2.0 mM MgCl₂ in 18.2 MΩ water (EMD Millipore) and the pH was adjusted to 7.40 using HCl. To produce an aCSF gelatin, porcine gelatin (0.5% by mass) was added and the solution was heated until it dissolved. The mixture was allowed to set at room temperature.

Parent K.L., Hill D.F., Crown L.M., Wiegand J.P., Gies K.F., Miller M.A., Atcherley C.W., Heien M.L, & Cowen S.L. (2017). Platform to Enable Combined Measurement of Dopamine and Neural Activity. Analytical chemistry, 89(5), 2790-2799.

+ Open protocol

+ Expand

Radioligand Binding Assay Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Materials: spiperone-d2, SNAP-Lumi4-Tb and 5x SNAP/CLIP labeling medium were purchased from Cisbio Bioassays. Eticlopride hydrochloride was purchased from Tocris Bioscience. Saponin was purchased from Fluka/Sigma-Aldrich. Bromocriptine, haloperidol, risperidone, spiperone, pluronic-F127, Gpp(NH)p, DNA primers, Hanks Balanced Salt Solution H8264 (HBSS) and phosphate buffered saline (PBS) was purchased from Sigma-Aldrich.

Lane J.R., Abramyan A.M., Adhikari P., Keen A.C., Lee K.H., Sanchez J., Verma R.K., Lim H.D., Yano H., Javitch J.A, & Shi L. (2020). Distinct inactive conformations of the dopamine D2 and D3 receptors correspond to different extents of inverse agonism. eLife, 9, e52189.

+ Open protocol

+ Expand

Pharmacological Preparation of Dopamine Agonists

Check if the same lab product or an alternative is used in the 5 most similar protocols

SKF83959 hydrobromide (Tocris Bioscience) was dissolved in the vehicle containing 10% ethanol in 0.9% physiological saline. SCH23390 hydrochloride (Tocris Cookson, Bristol, UK) and eticlopride hydrochloride (Tocris Bioscience) were dissolved in 0.9% physiological saline. The drug was intraperitoneally (i.p.) injected at a volume of 1 ml/kg of body weight.

Liu P.P., Chao C.C, & Liao R.M. (2021). Lack of effect of dopamine receptor blockade on SKF83959-altered operant behavior in male rats. The Chinese journal of physiology, 64(1).

+ Open protocol

+ Expand

Dopamine Receptor Modulation in Animal Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

SKF 81297 hydrobromide (Tocris Bioscience; D1R agonist) was administered systemically at doses of 0.3 mg/kg and 1.0 mg/kg, and locally at doses of 0.4 µg/µl and 4.0 µg/µl. SCH 23390 hydrochloride (Tocris Bioscience; D1R antagonist) was administered systemically at doses of 0.005mg/kg and 0.01mg/kg, and locally at doses of 0.2µg/µl and 2.0µg/µl. Quinpirole hydrochloride (Tocris Bioscience; D2R agonist) was administered systemically at doses of 0.0125 mg/kg and 0.0375 mg/kg, and eticlopride hydrochloride (Tocris Bioscience; D2R antagonist) was administered systemically at doses of 0.01 mg/kg and 0.03 mg/kg. Doses were calculated as the salt. All drugs were dissolved in 0.9% sterile saline, made in batch, aliquoted, and frozen at -20°C. Individual aliquots were defrosted for use on testing days. 0.9% sterile saline was administered in control sessions. In all experiments, doses were applied in a counterbalanced Latin square approach, although blinding was not used when making up or applying agents.

Grima L.L., Panayi M.C., Härmson O., Syed E., Manohar S., Husain M., & Walton M. (2021). Nucleus accumbens D1-receptors regulate and focus transitions to reward-seeking action.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!