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Anti human aβ fluorescent enzyme linked immunosorbent assay

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The Anti-human Aβ fluorescent enzyme-linked immunosorbent assay (ELISA) is a laboratory tool used to detect and quantify the presence of amyloid-beta (Aβ) proteins in biological samples. This assay utilizes fluorescent-labeled antibodies that specifically bind to human Aβ proteins, allowing for their detection and measurement through a fluorescent signal.

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Lab products found in correlation

Normal mouse serum, by Innovative Research (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)

2 protocols using anti human aβ fluorescent enzyme linked immunosorbent assay

1

Quantifying Amyloid-beta Secretion from Lens Explants

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Lenses (n = 4) were dissected from 10-week-old Tg2576 transgenic (Tg+) mice and age-matched non-transgenic (Tg−) littermate control mice (n = 4). One lens from each mouse was placed in a separate well containing tissue culture media (M199; Sigma-Aldrich, Burlington, MA) supplemented with 1% penicillin/streptomycin (Invitrogen/Thermo-Fisher Scientific, Waltham, MA) and 10% heat-inactivated normal mouse serum (Innovative Research, Southfield, MI). Cultures were incubated with humidification, 5% carbon dioxide, at 37 °C (Sanderson et al., 2000 (link)). Samples of conditioned media from cultures containing explanted intact lenses were collected after 2 days and 6 days of incubation. Media collected from cultures incubated without dissected lenses served as a negative control. Quantitative measurement of human Aβ species was performed on conditioned and control media samples using an anti-human Aβ fluorescent enzyme-linked immunosorbent assay (BioSource, Invitrogen, Carlsbad, CA).
Moncaster J.A., Moir R.D., Burton M.A., Chadwick O., Minaeva O., Alvarez V.E., Ericsson M., Clark J.I., McKee A.C., Tanzi R.E, & Goldstein L.E. (2022). Alzheimer’s disease amyloid-β pathology in the lens of the eye. Experimental eye research, 221, 108974.
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2

Quantifying Amyloid-beta Secretion from Lens Explants

Check if the same lab product or an alternative is used in the 5 most similar protocols
Lenses (n = 4) were dissected from 10-week-old Tg2576 transgenic (Tg+) mice and age-matched non-transgenic (Tg−) littermate control mice (n = 4). One lens from each mouse was placed in a separate well containing tissue culture media (M199; Sigma-Aldrich, Burlington, MA) supplemented with 1% penicillin/streptomycin (Invitrogen/Thermo-Fisher Scientific, Waltham, MA) and 10% heat-inactivated normal mouse serum (Innovative Research, Southfield, MI). Cultures were incubated with humidification, 5% carbon dioxide, at 37 °C (Sanderson et al., 2000 (link)). Samples of conditioned media from cultures containing explanted intact lenses were collected after 2 days and 6 days of incubation. Media collected from cultures incubated without dissected lenses served as a negative control. Quantitative measurement of human Aβ species was performed on conditioned and control media samples using an anti-human Aβ fluorescent enzyme-linked immunosorbent assay (BioSource, Invitrogen, Carlsbad, CA).
Moncaster J.A., Moir R.D., Burton M.A., Chadwick O., Minaeva O., Alvarez V.E., Ericsson M., Clark J.I., McKee A.C., Tanzi R.E, & Goldstein L.E. (2022). Alzheimer’s disease amyloid-β pathology in the lens of the eye. Experimental eye research, 221, 108974.
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