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Superoxide dismutase determination kit

Manufactured by Merck Group
Sourced in India, United States

The Superoxide dismutase determination kit is a laboratory equipment designed to measure the activity of the enzyme superoxide dismutase (SOD) in biological samples. The kit provides the necessary reagents and protocols to quantify SOD levels using a colorimetric or fluorometric assay method.

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4 protocols using superoxide dismutase determination kit

1

Oxidative Stress Biomarkers in Skeletal Muscle

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The levels of FFA, TG, SOD, GSH-Px, and MDA in skeletal muscle were determined with free fatty acid determination kit (Sigma), triglyceride determination kit (Sigma), superoxide dismutase determination kit (Sigma), and glutathione peroxidase determination kit (Sigma), respectively, following the manufacturer's instructions. The 8-hydroxyl-deoxyguanosine (8-OHdG) content was measured using enzyme-linked immunosorbent assay (ELISA) (R&D, Minneapolis, USA).
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2

Superoxide Dismutase Activity Assay

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The Superoxide Dismutase (SOD) assays were carried out by using the Superoxide dismutase determination kit (Sigma-Aldrich, Bangalore, India). Activity of SOD per 1 unit was resolute as the required enzyme to slab the increment absorbance by 50% at 440 nm.
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3

Oxidative Stress Biomarkers in Skeletal Muscle

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The levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA), in the skeletal muscle, were determined with superoxide dismutase determination kit (Sigma) and glutathione peroxidase determination kit (Sigma), respectively, following the manufacturer's instructions. The 8-hydroxyl-deoxyguanosine (8-OHdG) content was measured using enzyme-linked immunosorbent assay (ELISA) (R&D, Minneapolis, USA).
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4

Phytochemical and Antioxidant Assays

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We purchased 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), acetone, acetonitrile, citric acid, DPPH, ferrous-ion-o-dianisidine, Folin–Ciocalteu phenol reagent, formic acid, gallic acid, hydrochloric acid, methanol, β-nicotinamide adenine dinucleotide 2-phosphate reduced tetrasodium salt (NADPH), ultrapure phenolic standards, 4-amino-3-hydrazino-5-mercapto-1,2,4-triazole (Purpald), sodium acetate, superoxide dismutase determination kit, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), and 6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox) from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). Ascorbic acid, boric acid, ferric chloride, ferrous sulfate, hydrogen peroxide, potassium chloride, sodium carbonate, sodium hydroxide, and sulfuric acid were obtained from Vetec (Rio de Janeiro, RJ, Brazil). Cyanidin-3-O-glucoside, delphinidin-3-O-glucoside, delphinidin, quercetin-3-O-glucoside, 3-O-caffeoyl-quinic acid, and myricetin were purchased from Extrasynthese (Genay, France). HA, PCA, VA, isovanillic acid (iVA), ellagic acid, FA, and kaempferolwere purchased from Sigma-Aldrich (St Louis, MO, USA). The kit to analyze urine creatinine was purchased from Labtest Diagnóstica S.A. (Lagoa Santa, MG, Brazil).
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