Protein marker pagemark tricolor

Ampicillin and isopropyl-β-D-1-tiogalactopyranoside were from Apollo Scientific (Stockport, UK). Imidazole, kanamycin, TSP ((trimethylsilyl)-2,2,3,3-tetradeuteropropionic acid), Trizma base, and His-Select HF nickel resin were from Sigma-Aldrich (Madrid, Spain). Triton X-100 and protein marker (PAGEmark Tricolor) were from VWR (Barcelona, Spain). Amicon centrifugal devices with a cut-off molecular weight of 30 or 50 kDa were from Millipore (Barcelona, Spain). The rest of the materials were of analytical grade. Water was deionized and purified on a Millipore system.

Isopropyl-β-d-1-tiogalactopyranoside and ampicillin were obtained from Apollo Scientific (Stockport, UK). Imidazole, kanamycin, Trizma base, and His-Select HF nickel resin were from Sigma-Aldrich (Madrid, Spain). Protein marker (PAGEmark Tricolor) and Triton X-100 were from VWR (Barcelona, Spain). Amicon centrifugal devices were from Millipore (Barcelona, Spain), and they had a cut-off molecular weight of 30 or 50 kDa. The rest of the materials were of analytical grade. Water was deionized and purified on a Millipore system.

Ampicillin and isopropyl-β-D-1-tiogalactopyranoside were obtained from Apollo Scientific (Stockport, UK). Imidazole, Trizma base and His-Select HF nickel resin were from Sigma-Aldrich (Madrid, Spain). Triton X-100, and protein marker (PAGEmark Tricolor) were from VWR (Barcelona, Spain). The compound 5-fluorouracile (5-FU) was offered by Institute Paoli-Calmettes. Amicon centrifugal devices with a cut-off molecular weight of 3 kDa were from Millipore (Barcelona, Spain). The rest of the materials were of analytical grade. Water was deionized and purified on a Millipore system. Piperazine analogs, 2-(trifluoromethyl)phenothiazine and other reagents were purchased from Adamas-beta (Shanghai, China) or Energy Chemical (Shanghai, China).