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2 n morpholino ethanesulfonic acid sodium salt

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2-(N-morpholino)ethanesulfonic acid sodium salt is a zwitterionic buffering agent commonly used in biochemical and cell culture applications to maintain a stable pH environment. It is a white, crystalline powder that is highly soluble in water.

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Lab products found in correlation

Hydrochloric acid (hcl), by Thermo Fisher Scientific (3 mentions) Glycolaldehyde dimer, by Merck Group (2 mentions) Triethanolamine hydrochloride, by Merck Group (2 mentions) Sephacryl s 200, by GE Healthcare (2 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (2 mentions) Q sepharose, by GE Healthcare (2 mentions) Kanamycin sulfate, by Merck Group (1 mentions) Nicotinamideadenine dinucleotide reduced, by Merck Group (1 mentions) Ampicillin, by Merck Group (1 mentions) Dl dithiothreitol, by Merck Group (1 mentions) Acetaldehyde, by Merck Group (1 mentions) Dl dithiothreitol dtt, by Thermo Fisher Scientific (1 mentions) Sodium ascorbate, by Merck Group (1 mentions) Ha sodium salt, by Lifecore Biomedical (1 mentions) Copper 2 sulfate pentahydrate, by Thermo Fisher Scientific (1 mentions) Tris 3 hydroxypropyltriazolylmethyl amine, by Merck Group (1 mentions) N hydroxysuccinimide, by Thermo Fisher Scientific (1 mentions) N hydroxysuccinimide, by Tokyo Chemical Industry (1 mentions) 3 n morpholino propanesulfonic acid, by Merck Group (1 mentions) Gold nanospheres, by NanoComposix (1 mentions)

4 protocols using 2 n morpholino ethanesulfonic acid sodium salt

1

Purification and Enzyme Characterization

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Water
was purified using Milli-Q Academic
purification system. Q-Sepharose and Sephacryl S-200 were purchased
from GE Healthcare. Dowex 50WX4-200R (H+ form), nicotinamide
adenine dinucleotide reduced (NADH, disodium salt), dihydroxyacetone
phosphate hemimagnesium salt, glycolaldehyde dimer, 2-(N-morpholino)ethanesulfonic acid sodium salt (MES, ≥99.5%),
triethanolamine hydrochloride (TEA, ≥99.5%), ampicillin, kanamycin
sulfate, and d,l-dithiothreitol (DTT) were purchased
from Sigma-Aldrich. Protease inhibitor tablets (Complete brand) and
bovine serum albumin, fraction V (BSA), were purchased from Roche.
Ammonium sulfate (enzyme grade), guanidinium hydrochloride (electrophoresis
grade, min. 99%), sodium hydroxide (1.0 N), and hydrochloric acid
(1.0 N) were purchased from Fisher. Sodium phosphite (dibasic, pentahydrate)
was purchased from Fluka, and its water content was reduced to Na2HPO3·0.4H2O as previously described.7 (link) Quikchange II Site-Directed Mutagenesis Kits
were purchased from Agilent Technologies, and λDE3 Lysogenization
Kits were purchased from Novagen. All other chemicals were reagent
grade or better and were used without further purification.
He R., Reyes A.C., Amyes T.L, & Richard J.P. (2018). Enzyme Architecture: The Role of a Flexible Loop in Activation of Glycerol-3-phosphate Dehydrogenase for Catalysis of Hydride Transfer. Biochemistry, 57(23), 3227-3236.
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2

Purification and Characterization of Enzyme

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Water was obtained from
a Milli-Q Academic
purification system. Q-Sepharose and Sephacryl S-200 were purchased
from GE Healthcare. Dowex hydrogen form, nicotinamide adenine dinucleotide,
reduced form (NADH, disodium salt), glycolaldehyde dimer, 2-(N-morpholino)ethanesulfonic acid sodium salt (MES, ≥99.5%),
triethanolamine hydrochloride (≥99.5%), and acetaldehyde were
purchased from Sigma-Aldrich. Ethylammonium chloride, d,l-dithiothreitol (DTT), sodium hydroxide (1.0 N), and hydrochloric
acid (1.0 N) were purchased from Fisher Scientific. Sodium phosphite
dibasic form was purchased from Riedel de Haën. The λDE3
lysogenization kits were purchased from Novagen. All other chemicals
were reagent grade or better and were used without further purification.
Cristobal J.R, & Richard J.P. (2022). Glycerol-3-Phosphate Dehydrogenase: The K120 and K204 Side Chains Define an Oxyanion Hole at the Enzyme Active Site. Biochemistry, 61(10), 856-867.
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3

Synthesis and Characterization of Click-Functionalized Peptides

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We purchased HA sodium salt (molecular weight 16 kDa) from Lifecore Biomedical; 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride and N-hydroxysuccinimide from TCI Chemicals; 2-(N-morpholino)ethane-sulfonic acid sodium salt, tris(3-hydroxypropyltriazolylmethyl)amine, and sodium ascorbate from Sigma-Aldrich; and 11-azido-3,6,9-trioxaundecan-1-amine, N-hydroxysuccinimide, and copper (II) sulfate pentahydrate from Acros Organics. Alkyne-functionalized peptides bearing an N-terminal 4-pentynoic acid (homopropargyl) modification, hpP79 and aoP79, were obtained from Biomatik. Additionally, alkyne-functionalized peptides bearing an N-terminal alkyne polyethylene glycol group modification, 2.5HIP and a control p79 with two permuted amino acids inactive on BDC2.5 T cells, were purchased from PolyPeptide. All reagents were used as received without further purification.
Firdessa-Fite R., Johnson S.N., Leon M.A., Khosravi-Maharlooei M., Baker R.L., Sestak J.O., Berkland C, & Creusot R.J. (2021). Soluble Antigen Arrays Efficiently Deliver Peptides and Arrest Spontaneous Autoimmune Diabetes. Diabetes, 70(6), 1334-1346.
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4

Immobilization of Antibody on Gold Nanoparticles

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N-(3-dimethylaminopropyl)-N′-carbodiimide (EDC, 97%), N-hydroxysuccinimide
(NHS, 98%), 3-(N-morpholino)propanesulfonic acid
(MOPS, 99.5%), 3-(N-morpholino)propanesulfonic acid
sodium salt (99.5%), 2-(N-morpholino)ethanesulfonic
acid (MES, 99.5%), 2-(N-morpholino)ethanesulfonic
acid sodium salt (99%), hydroxylamine 50 weight % in H2O, isopropyl alcohol (IPA, 98%) were purchased from Sigma-Aldrich
(St. Louis, MO). Hydrochloric Acid, 10% v/v aqueous solution (HCl),
was purchased from Alfa Aesar (Ward Hill, MA). Gold nanospheres (40
nm, BioReady, carboxyl, 20 OD, 5 mL, water) were obtained from NanoComposix
(San Diego, CA). A rabbit polyclonal antibody against L. monocytogenes (PIPA130487) was purchased from
Fisher Scientific and used without further purification. All reagents
were used as received. Ultrapure type-1 water (18 MΩ cm) was
acquired from an Elga PURELAB purification system and was used for
all buffer preparations. Thermo Scientific Pierce 96-Well Plates,
Product No. 15041 was used for bicinchoninic acid (BCA) assay. Bovine
serum albumin (BSA) standard kit was purchased from Thermo Fisher
catalog #23225.
Busch R.T., Karim F., Weis J., Sun Y., Zhao C, & Vasquez E.S. (2019). Optimization and Structural Stability of Gold Nanoparticle–Antibody Bioconjugates. ACS Omega, 4(12), 15269-15279.
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