Ultimate 3000 uhplc instrument

Manufactured by Thermo Fisher Scientific

Sourced in United States

The UltiMate 3000 UHPLC instrument is a high-performance liquid chromatography system designed to provide efficient and accurate separation and analysis of a wide range of chemical compounds. The instrument features advanced technology and components to deliver reliable and reproducible results.

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Lab products found in correlation

Silica gel, by Qingdao Haiyang Chemical (4 mentions) Sephadex lh 20, by GE Healthcare (3 mentions) 500 mhz nmr spectrometer, by Bruker (2 mentions) Ascend 600 mhz, by Bruker (2 mentions) Varian cary 50 uv vis, by Agilent Technologies (2 mentions) Esi q orbitrap ms mass spectrometer, by Thermo Fisher Scientific (2 mentions) Rudolph autopol 4 automatic polarimeter, by Rudolph Research Analytical (2 mentions) Varian 640 ir ft ir spectrophotometer, by Agilent Technologies (2 mentions) Cary 50 uv vis, by Agilent Technologies (1 mentions) 640 ir ftir spectrophotometer, by Agilent Technologies (1 mentions) Mci gel chp 20p, by Mitsubishi Chemical Corporation (1 mentions) Cosmosil pbr, by Nacalai Tesque (1 mentions) Acquity uplc hss c18, by Waters Corporation (1 mentions) Chromeleon software, by Thermo Fisher Scientific (1 mentions) Isq ec mass spectrometer, by Thermo Fisher Scientific (1 mentions) Mci gel, by Mitsubishi Chemical Corporation (1 mentions) Ascend 500 mhz nmr spectrometer, by Bruker (1 mentions)

Spelling variants of this product

Ultimate 3000 (1640 citations) UltiMate 3000 UHPLC (232 citations) Ultimate 3000 instrument (3 citations) Dionex Ultimate 3000 UHPLC+ instrument (3 citations) UHPLC instrument (2 citations)

6 protocols using ultimate 3000 uhplc instrument

Characterization of Phytochemical Compounds

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UV and IR spectra were recorded on a Varian Cary 50 UV-Vis and Varian 640-IR FT-IR spectrophotometer, respectively. Optical rotations were measured on a Rudolph Autopol^® IV automatic polarimeter. NMR spectra were determined on a Bruker 500 MHz NMR spectrometer at 500 MHz for ¹H and 125 MHz for ¹³C-NMR (internal standard: TMS). Negative-ion mode ESI-Q-Orbitrap MS were obtained on a Thermo UltiMate 3000 UHPLC instrument (Thermo, Waltham, MA, USA).
Column chromatographies (CC) were performed on macroporous resin D101 (Haiguang Chemical Co., Ltd., Tianjin, China), silica gel (48–75 μm, Qingdao Haiyang Chemical Co., Ltd., Qingdao, China), ODS (40–63 μm, YMC Co., Ltd., Tokyo, Japan), and Sephadex LH-20 (Ge Healthcare Bio-Sciences, Uppsala, Sweden). Preparative high performance liquid chromatography (pHPLC) column, Cosmosil 5C₁₈-MS-II (20 mm i.d. × 250 mm, Nakalai Tesque, Inc., Tokyo, Japan) were used to separate the constituents.

Dong Y., Ruan J., Ding Z., Zhao W., Hao M., Zhang Y., Jiang H., Zhang Y, & Wang T. (2020). Phytochemistry and Comprehensive Chemical Profiling Study of Flavonoids and Phenolic Acids in the Aerial Parts of Allium Mongolicum Regel and Their Intestinal Motility Evaluation. Molecules, 25(3), 577.

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Phytochemical Analysis of Natural Compounds

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NMR spectra were performed on Bruker ascend 600 MHz and/or Bruker ascend 500 MHz NMR spectrometer (Bruker BioSpin AG Industriestrasse 26 CH-8117) with tetramethylsilane as an internal standard. Negative-ion mode ESI-Q-Orbitrap MS were determined on a Thermo ESI-Q-Orbitrap MS mass spectrometer connected to the UltiMate 3000 UHPLC instrument via ESI interface (Thermo Scientific). Optical rotations, UV, IR, and ECD spectra were run on a Rudolph Autopol^® IV automatic polarimeter (l = 50 mm) (Rudolph Research Analytical, Hackettstown), Varian Cary 50 UV-Vis (Varian, Inc.), Varian 640-IR FT-IR spectrophotometer (Varian Australia Pty Ltd.), and Circular dichroism spectrum (J-815, JASCO company), respectively.
Column chromatorgtaphies (CC) were accomplished on macroporous resin D101 (Haiguang Chemical Co., Ltd.), silica gel (48–75 μm, Qingdao Haiyang Chemical Co., Ltd.), ODS (50 μm, YMC Co., Ltd.), MCI gel CHP 20P (Mitsubishi Chemical Corporation, CHP20/P120), and Sephadex LH-20 (Ge Healthcare Bio-Sciences). HPLC column: Cosmosil 5C18-MS-II (4.6 mm i. d. × 250 and 20 mm i. d. × 250 mm) (5 μm, Nakalai Tesque, Inc.) were used to analysis and separate the constituents, respectively.
Dichloromethane (CH₂Cl₂), methanol (MeOH), acetonitrile (CH₃CN), acetic acid (HAc), and other reagents (chromatographically pure or analytical pure) were purchased from Tianjin Concord Technology Co., Ltd.

Yan J., Hao M., Han Y., Ruan J., Zheng D., Sun F., Cao H., Hao J., Zhang Y, & Wang T. (2021). Sesquiterpenes From Oplopanax elatus Stems and Their Anti-Photoaging Effects by Down-Regulating Matrix Metalloproteinase-1 Expression via Anti-Inflammation. Frontiers in Chemistry, 9, 766041.

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Analytical Techniques for Compound Characterization

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Optical rotations were measured on a Rudolph Autopol® IV automatic polarimeter (l = 50 mm) (Rudolph Research Analytical, Hackettstown NJ, USA). NMR spectra were determined on a Bruker 500-MHz NMR spectrometer (Bruker BioSpin AG Industriestrasse 26 CH-8117, Fällanden, Switzerland) at 500 MHz for ¹H and 125 MHz for ¹³C NMR (internal standard: TMS). IR spectra were recorded on a Varian 640-IR FT-IR spectrophotometer (Varian Australia Pty Ltd., Mulgrave, Australia). Negative-ion mode ESI-Q-Orbitrap-MS was obtained on a Thermo ESI-Q-Orbitrap MS mass spectrometer connected with the UltiMate 3000 UHPLC instrument via ESI interface (Thermo Fisher Scientific, Inc., Waltham, MA, USA).
CC was performed on macroporous resin D101 (Haiguang Chemical Co., Ltd., Tianjin, China), silica gel (48–75 μm, Qingdao Haiyang Chemical Co., Ltd., Qingdao, China), and ODS (40–63 μm, YMC Co., Ltd., Tokyo, Japan). pHPLC columns, Cosmosil 5C₁₈-MS-II (20 mm i.d. ×250 mm, Nacalai Tesque, Inc., Kyoto, Japan), and Cosmosil PBr (20 mm i.d. ×250 mm, Nacalai Tesque, Inc., Kyoto, Japan) were used to separate the constituents.

Chen Y., Ruan J., Sun F., Wang H., Yang S., Zhang Y., Yan J., Yu H., Guo Y., Zhang Y, & Wang T. (2020). Anti-inflammatory Limonoids From Cortex Dictamni. Frontiers in Chemistry, 8, 73.

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UHPLC Separation of Organic Compounds

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A Thermo UltiMate 3000 UHPLC instrument (Thermo, Waltham, MA) equipped with a quaternary pump, an autosampler was used to accomplish the analysis. Samples were separated on a Waters ACQUITY UPLC® HSS C18 (2.1 × 100 mm, 1.8 μm) using a mobile phase composed of H₂O with 0.1% formic acid (A) and CH₃CN with 0.1% formic acid (B) in the gradient program: 0–2 min, 9–10% B; 2–5 min, 10–17% B; 5–7 min, 17–20% B; 7–9 min, 20% B; 9–10 min, 20–86% B; 10–14 min, 86–100% B; 14–17 min, 100% B; An equilibration of 3 min was used between successive injections. The flow rate was 0.4 mL/min, and column temperature was 35 °C. An aliquot of 1 μL of each sample was injected for analysis.

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Quantitative LC-MS Profiling of Analytes

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LC–MS analyses were performed by an Ultimate 3000 UHPLC instrument coupled with an ISQ EC mass spectrometer, equipped with an electrospray ion source and a single-quadrupole analyzer (Thermo Fisher Scientific, Cambridge, MA, USA). A C18, Luna® Phenomenex, 5 µm 100 Å (150 × 4.6 mm) (Phenomenex, Torrence, CA, USA) was used for chromatographic separation. The mobile phase was composed of solvents A (Water, 0.1% HCOOH) and B (Acetonitrile, 0.1% HCOOH). An isocratic mode (30:70) was used. The flow rate was set at 0.2 mL/min and the column was maintained at 30 °C for the entire run. Fifty microliters were injected. Analyses were performed using the positive ionization mode selecting the following m/z: 91, 137, 154, 369. The collision energy (CE) was 20 eV. Mass spectrometry parameters were: spray voltage (V) 3000, sheet gas (arb) 28.8 psig, aux gas (arb) 3.2 psig, ion transfer tube temperature 300 °C, and vaporizer temp 117 °C. Peak areas were measured by using the Chromeleon software (Thermo Fisher Scientific).

Vázquez Alberdi L., Martínez-Busi M., Arrarte E., Echeverry C., Calero M, & Kun A. (2024). A low dose of curcumin-PDA nanoparticles improves viability and proliferation in endoneurial fibroblasts and Schwann cell cultures. Discover Nano, 19(1), 81.

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Analytical Techniques for Compound Characterization

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The following instruments were used to obtain physical data—NMR spectra were measured on Bruker ascend 600 MHz or a Bruker ascend 500 MHz NMR spectrometer (Bruker BioSpin AG Industriestrasse 26 CH-8117, Fällanden, Switzerland) with tetramethylsilane as an internal standard. The negative-ion mode ESI-Q-Orbitrap MS was obtained on a Thermo UltiMate 3000 UHPLC instrument (Thermo, Waltham, MA, USA). Optical rotations, UV, and IR spectra were run on a Rudolph Autopol^® IV automatic polarimeter (l = 50 mm) (Rudolph Research Analytical, Hackettstown NJ, USA), Varian Cary 50 UV–Vis (Varian, Inc., Hubbardsdon, MA, USA) and Varian 640-IR FT-IR spectrophotometer (Varian Australia Pty Ltd., Mulgrave, Australia), respectively.
CC were performed on macroporous resin D101 (Haiguang Chemical Co., Ltd., Tianjin, China), silica gel (48–75μm, Qingdao Haiyang Chemical Co., Ltd., Qingdao, China), ODS (50 μm, YMC Co., Ltd., Tokyo, Japan), MCI gel (Mitsubishi Chemical Corporation, Osaka, Japan), and Sephadex LH-20 (Ge Healthcare Bio-Sciences, Uppsala, Sweden). High performance liquid chromatography (HPLC) column—Cosmosil 5C₁₈-MS-II (4.6 mm i.d. × 250 mm, 5 µm) and Cosmosil 5C₁₈-MS-II (20 mmi.d. × 250 mm, 5 µm, NakalaiTesque, Inc., Tokyo, Japan) were used to analyze and separate the constituents.

Sun F., Ruan J., Zhao W., Zhang Y., Xiang G., Yan J., Hao M., Wu L., Zhang Y, & Wang T. (2019). New Dammarane-Type Triterpenoid Saponins from Panax notoginseng Leaves and Their Nitric Oxide Inhibitory Activities. Molecules, 25(1), 139.

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