Hrp conjugated anti rabbit antibody w4011

Ultrapure water (pH 6.7; Milli-Q, Bedford, MA) was used in all situations throughout the experiments. Sodium tetrachloropalladate (II) (Na₂PdCl₄), decylamine (DA), 3-Methyladenine (M9281), bafilomycin A1 (B1793), Chloroquine (C6628), MitoTEMPO (SML0737) and VAS2870 (SML0273) were purchased from Sigma-Aldrich Co., LLC. (Shanghai, China). Copper (I) chloride (CuCl), poly(vinyl pyrrolidone) (PVP, Mw≈30000), glucose (α or β form), N,N-dimethylformamide (DMF), and ethanol (C₂H₆O) were purchased from Sinopharm Chemical Reagent Co. Lid. (Shanghai, China). All chemicals were used as received without further purification. Anti-LC3 antibody (NB100–2220, 1:2000 dilution) were purchased from Novus Biologicals. Anti-GAPDH antibodies (AB9132, 1:10,000 dilution) were from Chemicon.,and anti-ATG5 antibody (sc-133158, 1:500 dilution) were from Santa Cruz Biotechnology. HRP-conjugated anti-rabbit antibody (W4011, 1:10,000 dilution) and HRP-conjugated anti-mouse antibody (W4021, 1:10,000 dilution) were purchased from Promega. The HeLa, 4T1, MCF7 cell lines were purchased from ATCC. MCF-7/MDR (kindly provided by Dr Jun Wang at South China University of Technology) were maintained in the DMEM medium containing doxorubicin.

Anti-TRPV1 (1:100, ab6166), anti-LC3 (1:1000, ab192890), anti-P62 (1:1000, ab109012), anti-LAMP2 (1:100, ab13524), anti-H2 Db/H2-D1(1:100, ab25244), and Alexa Fluor 568 (1:500), Alexa Fluor 488 (1:500) secondary antibody were purchased from Abcam. Anti-CGRP (1:100, sc-57053) was purchased from Santa cruze. Anti-mouse CD45-PE-cy7 (103114), anti-mouse CD11c-APC-cy7 (117323), anti-mouse CD3-APC-cy7 (100221), anti-mouse CD8a-APC (100711), anti-mouse CD16/32-TruStain FcX™ (101320), anti-mouse CD86-APC (105011) antibodies were purchased from BioLegend. APC anti-mouse H-2 (E-AB-F1216E) and Anti-mouse CD80-FITC (E-AB-F0992C) were purchased from Elabscience. HRP-conjugated anti-rabbit antibody (W4011) and HRP-conjugated anti-mouse antibody (W4021) were purchased from Promega. Enhanced chemiluminescence (ECL) kit was purchased from Biological Industries. One Step TUNEL Apoptosis Assay Kit (Green Fluorescence) (C1088) and Ad-GFP-LC3B (C3006) were purchased from Beyotime. Lyso-Tracker Red (L12492) was purchased from Invitrogen. Imiquimod (HY-B0180A) was purchased from MCE. Indocyanine Green (3599-32-4) was purchased from Aladdin. Morphine (161007-2) was purchased from NORTHEAST PHARM. Ropivacaine (R413090) was purchased from Mackline. Pluronic F127 (P2443) was purchased from Sigma.

Anti-Caspase-3 antibody (#9662), anti-PARP antibody (#9542), anti-phospho-Chk1 (Ser296) antibody (#2349), anti-phospho-Chk1 (Ser317) antibody (#2344), anti-phospho-Chk1 (Ser345) antibody (#2348), anti-Chk1 antibody (#2345), anti-phospho-Chk2 (Thr68) antibody (#2661), anti-phospho-Chk2 (Ser516) antibody (#2669), anti-Chk2 antibody (#2662), anti-phospho-p53 (Ser15) antibody (#9284), and anti-phospho-p53 (Ser20) antibody (#9287) were obtained from Cell Signaling Technology; anti-p53 antibody (DO-1; sc-126), anti-PRODH antibody (sc-376401), and HRP-conjugated anti-rat antibody (sc-2032) were from Santa Cruz Biotechnology; anti-α-tubulin antibody (T9026) and anti-γ-tubulin antibody (T6557) were from Sigma Aldrich; anti-p21 antibody (K0081-3) and anti-ATM antibody (PM026) were from Medical and Biological Laboratories; HRP-conjugated anti-rabbit antibody (W4011) and HRP-conjugated anti-mouse antibody (W4021) were from Promega; anti-HA antibody (1867423) was from Roche; anti-DAO antibody (6844-1) was from Abcam.