Calnexin h 70

C2C12 mouse cells were purchased from the European Collection of Animal Cell Cultures (ECACC), reagents from Sigma Chemical Co. (St. Louis-MO, USA).
Primary antibodies against: Calnexin (H-70), GAPDH (FL-335), AKT (C-20), CaMKII (M-176), pCaMKIIalpha (Thr286), MyHC (H-300), Myf5 (C-20), MyoD (C-20), Myogenin (D-10), p53 (FL-393), p70S6 (C-18), pp70S6 (C-18), ERK1 (K-23), ERK2 (C-14), pERK1/2 (E-4), p21 (C-19), OPN (K-20), peroxidase-conjugated secondary antibodies for Western blot analysis and Rhodamine-conjugated antibodies for Immunofluorescence studies were purchased from Santa Cruz Biotechnology (Santa Cruz-CA, USA). Primary antibody Phospho-AKT (Ser473-D9E-XP^TM) was purchased from Cell Signaling Technology (Danvers-MA, USA).
Fluorescently-labeled phalloidin (AlexaFluor^®488-Invitrogen) was purchased from Life Technologies (Carlsbad-CA, USA).
CytoPainter Mitochondrial Staining Kit-Green (AB 112143) was purchased from Prodotti Gianni (Milano-Italy), Cell ROX^® Oxidative Stress Reagents Kit (C10443) from Thermo Fisher Scientific, Life Technologies Italia (Monza-Italy).

Whole-cell lysates were prepared by washing cells with PBS, pelleting, then lysing with radioimmunoprecipitation assay (RIPA) buffer as described previously 6 (link). Equal protein of cell lysates and EV samples were measured by Pierce 660 nm Protein Assay (Invitrogen, 22,662) then loaded into an SDS 10% or 12% polyacrylamide gel. Western blot analysis was performed as described 57 . Ponceau S stain (Sigma, P7170) was used to visualize total protein. Blots were probed with the following antibodies: TSG101 (4A10; Genetex), CD63 (TS63; Abcam), CD9 (MM2/57; Millipore), Syntenin-1 (S-31; Santa Cruz), Calnexin (H-70; Santa Cruz), Aβ (2454; Cell Signaling), GFAP (PA5-16291, ThermoFisher), rabbit anti-mouse IgG (Genetex, GTX213112-01), goat anti-rabbit IgG (Genetex, GTX213110-01). Blots were imaged using a Bio-Rad ChemiDoc imager and processed using ImageQuant TL v8.1.0.0 software and CorelDraw Graphic Suite X5.

The following primary antibodies were used: anti-Aβ mouse monoclonal antibody 6E10 (MAB5206; Chemicon), anti-mouse S100A9 and S100A8 (AF2065, AF3059; R&D systems), S100B (ab52642;Abcam), GAPDH (Abfrontier), anti-Amyloid Oligomer, Aβ, (AB9234; Millipore),p-Tau (Ser404) (sc-12952;Santa Cruz Biotech.), Phospho-PHF-tau (S202/T205, AT8) (NM1020;Pierce), Anti-PhosphoTau (S396;PHF-13) (ab24716;Abcam), Tau (C-17) (sc-1995;Santa Cruz Biotechnology), Calnexin (H-70) (sc-11397;Santa Cruz Biotechnology), and BACE (M-83) (sc-10748;Santa Cruz Biotechnology).