Cbl b

Manufactured by Proteintech

Sourced in United States

Cbl-b is a laboratory protein product provided by Proteintech. It is an E3 ubiquitin-protein ligase that regulates various cellular processes. The core function of Cbl-b is to mediate the ubiquitination and subsequent proteasomal degradation of target proteins.

Automatically generated - may contain errors

Lab products found in correlation

Bca protein analysis kit, by Beyotime (1 mentions) Sds polyacrylamide gel electrophoresis, by Beyotime (1 mentions) Beyoecl star reagent, by Beyotime (1 mentions) Ripa buffer, by Beyotime (1 mentions) P nf κb p65, by Proteintech (1 mentions)

2 protocols using cbl b

Protein Quantification and Immunoblotting Protocol

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Cells or tissues were lysed with RIPA buffer (Beyotime, Hangzhou, China) containing a mixture of protease inhibitors. The total protein was quantified using BCA protein analysis kit (Beyotime). SDS-polyacrylamide gel electrophoresis (Beyotime) was used to separate the protein cleavage products. The main monovalent antibodies used were as follows: Cbl-b (1:500, Proteintech, Chicago, IL, USA), Atrogin-1 (1:1000, Bioss Antibodies, Beijing, China), MuRE-1 (1:1000, Proteintech). Protein was detected with BeyoECL Star reagent (Beyotime).

Yang X., Li Z., Wang Z., Yu J., Ma M, & Nie Q. (2022). miR-27b-3p Attenuates Muscle Atrophy by Targeting Cbl-b in Skeletal Muscles. Biomolecules, 12(2), 191.

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Protein Extraction and Western Blotting

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Total protein was extracted from the suitably treated cells and murine tissues using the RIPA lysis buffer. Western blotting was performed as per standard protocols using antibodies against CBLB, PI3K, AKT, NF-κB, and p-NF-κB p65 (Proteintech, USA).

Han S., Li X., Liu J., Zou Z., Luo L., Wu R., Zhao Z., Wang C, & Shen B. (2020). Bta-miR-223 Targeting CBLB Contributes to Resistance to Staphylococcus aureus Mastitis Through the PI3K/AKT/NF-κB Pathway. Frontiers in Veterinary Science, 7, 529.

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