Cells were stained with LIVE/DEAD Fixable Near-IR Dead Cell Stain kit (Invitrogen) and surface antibodies for 30 min at 4 °C. For intracellular cytokine staining, cells were treated with 50 nM phorbol-12-myristate-13-acetate (MilliporeSigma) and 250 nM ionomycin (MilliporeSigma) for 4 hours in the presence of Brefeldin A (BD Biosciences) before harvesting. Cells were washed and fixed with BD Cytofix™ Fixation Buffer (BD Biosciences) for 10 min at RT, then washed with PBS. Intracellular cytokines were stained in permeabilization buffer (eBioscience) for 30 min at 4 °C. The following antibodies were used: anti-LAG-3 (11C3C65, BioLegend), anti-PD-1 (EH12.1, BD Biosciences), anti-TIGIT (MBSA43, eBioscience), anti-Tim-3 (F38–2E2, Biolegend), anti-IFN-γ (4S.B3, eBioscience), and IL-10 (JES3–9D7, Biolegend). Cells were acquired on a BD Fortessa flow cytometer and data was analyzed with FlowJo software v10 (Threestar).
Anti tigit mbsa43
Manufactured by Thermo Fisher Scientific
Anti-TIGIT (MBSA43) is a mouse monoclonal antibody that binds to the TIGIT (T-cell immunoreceptor with Ig and ITIM domains) protein. TIGIT is an inhibitory receptor expressed on various immune cells, including T cells, NK cells, and regulatory T cells. The Anti-TIGIT (MBSA43) antibody can be used for research purposes to study the role of TIGIT in immune regulation and its potential therapeutic applications.
Automatically generated - may contain errors
Lab products found in correlation
Permeabilization buffer, by Thermo Fisher Scientific (2 mentions)
Anti ifn γ 4s b3, by Thermo Fisher Scientific (2 mentions)
Il 10 jes3 9d7, by BioLegend (2 mentions)
Anti tim 3 f38 2e2, by BioLegend (2 mentions)
Anti lag 3 11c3c65, by BioLegend (2 mentions)
Anti pd 1 eh12, by BD (2 mentions)
Cytofix fixation buffer, by BD (2 mentions)
Live dead fixable near ir dead cell stain kit, by Thermo Fisher Scientific (2 mentions)
Ionomycin, by Merck Group (2 mentions)
Phorbol 12 myristate 13 acetate, by Merck Group (2 mentions)
Brefeldin a, by BD (2 mentions)
2 protocols using anti tigit mbsa43
1
Multiparameter Immune Profiling of Cells
2
Phenotypic Profiling of Immune Cells
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Cells were stained with LIVE/DEAD Fixable Near-IR Dead Cell Stain kit (Invitrogen) and surface antibodies for 30 min at 4 °C. For intracellular cytokine staining, cells were treated with 50 nM phorbol-12-myristate-13-acetate (MilliporeSigma) and 250 nM ionomycin (MilliporeSigma) for 4 hours in the presence of Brefeldin A (BD Biosciences) before harvesting. Cells were washed and fixed with BD Cytofix™ Fixation Buffer (BD Biosciences) for 10 min at RT, then washed with PBS. Intracellular cytokines were stained in permeabilization buffer (eBioscience) for 30 min at 4 °C. The following antibodies were used: anti-LAG-3 (11C3C65, BioLegend), anti-PD-1 (EH12.1, BD Biosciences), anti-TIGIT (MBSA43, eBioscience), anti-Tim-3 (F38-2E2, Biolegend), anti-IFN-γ (4S.B3, eBioscience), and IL-10 (JES3-9D7, Biolegend). Cells were acquired on a BD Fortessa flow cytometer and data was analyzed with FlowJo software v10 (Threestar).
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