After staining the cells as above, the cells were dissociated with 0.25% trypase then analyzed using a Guava easyCyte System 8 (Millipore 25801, CA, USA).
Guava easycyte system 8
Manufactured by Merck Group
Sourced in United States
The Guava easyCyte System 8 is a compact flow cytometry system designed for basic cell analysis. It features eight detection channels and supports a variety of fluorescent dyes and markers. The system is capable of analyzing small sample volumes and provides basic cell counting and cell population identification functionalities.
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3 protocols using guava easycyte system 8
1
Cell Dissociation and Flow Cytometry
2
Mitochondrial Function and Apoptosis Assay
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Mitochondrial membrane potential was measured by fluorescence levels upon staining with JC-1 (Invitrogen, Cat M7514) and MitoTracker Deep Red (Invitrogen, Cat M22426) at 0.5 μg/ml for 30 min at 37°C according to the manufacturer’s instructions. Apoptosis of cells was assessed by staining cells with Annexin V/PI (Invitrogen, Cat V13242) at 37°C for 30 min according to the manufacturer’s instructions. The cells were then washed with PBS and resuspended in cold PBS containing 1% FBS for flow cytometric analyses with Guava easyCyte System 8 (Millipore 25801, Hayward, CA, United States).
3
Apoptosis Assay Using AV-PI Kit
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The apoptosis of cells was detected using an AV-PI kit, following the manufacturer’s protocols (Vazyme, Annexin V–FITC/PI Apoptosis Detection Kit). The cells were incubated with AV-PI for 10 min, washed with PBS, and then analyzed using a Guava easyCyte System 8 (Millipore 25801, CA, United States).
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