Cellulase from a niger

Manufactured by Merck Group

Sourced in Ireland

Cellulase from (A) niger is an enzyme complex that hydrolyzes the beta-1,4-glycosidic bonds in cellulose, the primary structural component of plant cell walls. It is produced by the filamentous fungus Aspergillus niger.

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Lab products found in correlation

β glycosidase, by Merck Group (1 mentions) Aglycones, by Merck Group (1 mentions) α l rhamnosidase, by Megazyme (1 mentions) L rhamnosus gg, by American Type Culture Collection (1 mentions) Mannan from s cerevisiae, by Merck Group (1 mentions) Ajmaline, by Merck Group (1 mentions) Hplc grade acetonitrile, by Merck Group (1 mentions) Ajmalicine, by Merck Group (1 mentions) α solanine, by Merck Group (1 mentions) Pectinase from aspergillus niger, by Merck Group (1 mentions)

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Cellulase (138 citations) Cellulase from (2 citations)

3 protocols using cellulase from a niger

Probiotic Strain Characterization Protocol

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Acetonitrile was purchased from Promochem (Wesel, Germany), recombinant α-l-rhamnosidase from prokaryotic source (190 U/mg) was obtained in purified form from Megazyme (Wicklow, Ireland), β-glycosidase/α-l-rhamnosidase mixture [Cellulase from (A) niger, 0.45 U/mg] from Sigma–Aldrich (St. Louis, MO, USA), flavonoid glycosides, aglycones and all other chemicals from Sigma–Aldrich [or Merck (Darmstadt, Germany)]. Dried powder of C. johnstonii rhizomes (voucher specimen No 010112, gift of Chiang Mai University, Thailand) was extracted with ethanol for 24 h, filtrated and evaporated to obtain a dried crude extract. The probiotic strains used in this study included Lactobacillus (L.) paracasei ssp. paracasei CRL 431 (ATCC 55544), L. paracasei ssp. paracasei (DN114001), L. paracasei ssp. paracasei DSM 20312 (Shirota), L. rhamnosus GG (ATCC 53103), L. reuteri (ATCC 55730), L. acidophilus LA-5 (DSM 13241), Bifidobacterium (B.) animalis ssp. lactis BB12 (DSM 15954), (B) longum ssp. infantis, L. plantarum (ATCC 15697), L. brevis (ATCC 367), L. delbrueckii ssp. bulgaricus (DSM 20081), Lactococcus (Lc.) lactis ssp. lactis (SR 3.54; NCIMB 30117), L. fermentum, Streptococcus (S.) salivarius ssp. thermophilus (ATCC 19258). Man–Rogosa–Sharpe (MRS) liquid medium was prepared as described previously [22 (link)].

Mueller M., Zartl B., Schleritzko A., Stenzl M., Viernstein H, & Unger F.M. (2017). Rhamnosidase activity of selected probiotics and their ability to hydrolyse flavonoid rhamnoglucosides. Bioprocess and Biosystems Engineering, 41(2), 221-228.

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Enzymatic Extraction and HPLC Analysis

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Cellulase, from A. niger and mannan from S. cerevisiae, were purchased from Sigma Chemicals Co., ajmaline, ajmalicine and α-solanine as high-performance liquid chromatography (HPLC) reference standards were also purchased from Sigma Co., HPLC grade acetonitrile bought from Merck.

Srivastava M., Sharma S, & Misra P. (2016). Elicitation Based Enhancement of Secondary Metabolites in Rauwolfia serpentina and Solanum khasianum Hairy Root Cultures. Pharmacognosy Magazine, 12(Suppl 3), S315-S320.

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Chromosome Preparation from Root Tips

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Root tips were collected from potted plants, pre-treated overnight at 4°C in 2mM 8hydroxyquinoline (BDH Chemicals), and then fixed in 3:1 (v/v) ethanol : acetic acid for 24 hr at 4°C (Bailey & Stace, 1992) . Next, root tips were digested in 10mM citrate buffer containing 25U/ml pectinase from Aspergillus niger (Sigma-Aldrich), 20U/ml cellulase from A. niger (Sigma-Aldrich) and 20 U/ml cellulase 'Onozuka R-10' from Trichoderma viride (Duchefa Biochemie). The digested roots were then dissected and squashed in 45% (v/v) acetic acid (Schwarzacher et al., 1989) . Chromosome preparations were preserved by quick-freezing on dry ice (Conger & Fairchild, 1953) , and mounted in VECTASHIELD® Mounting Medium with DAPI, before being observed and imaged on a Nikon Eclipse Ci fluorescence microscope.

Wilcox M.M., Desjardins S.D., & Stace C.A. (2021). Survey of the nothogenus xElyhordeum, with the description of a new nothospecies. British & Irish Botany, 3(1).

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