Gst 10000 0 ap

The proteasome inhibitor MG132 and protein synthesis inhibitor cycloheximide (CHX) were purchased from Selleck. Antibodies used in immunoblotting: eEF2K (ab45168, ab85721, 1:1000) was purchased from Abcam; PD-L1 (17952-1-AP, 1:1000), GSK3β (22104-1-AP, 1:2000), β-actin (60008-1-lg, 1:5000), Flag (66008-3-lg, 1:5000), tubulin (11224-1-AP, 1:5000), GST (10000-0-AP, 1:4000) were purchased from Proteintech; HA (No. 3724, 1:1000), p-GSK3β/S9 (No. 9323, 1:1000) were purchased from Cell Signaling Technologies; phosphoserine monoclonal antibody (mAb) (CSB-MA080235, 1:1000) was purchased from CUSABIO. Normal IgG/Peroxidase-conjugated AffiniPure Goat Anti-Rabbit/Mouse IgG (H+L) was purchased from Jackson Immuno Research.

Antibodies against glutathione-S-transferase (GST, 10000-0-AP), poly (ADP-ribose) polymerase 1 (PARP1, 13371-1-AP), Flag (20543-1-AP), MYC-tag (16286-1-AP), GFP (66002-1-Ig), Ki-67 (27309-1-AP), histone-H3 (17168-1-AP), topoisomerase 1 (TOP1, 20705-1-AP), and caspase 3 (19677-1-AP) were purchased from Proteintech (Wuhan, China); PAR (AM80), β-actin (A5441), and methyl methanesulfonate (MMS, M4016) were obtained from Sigma–Aldrich (St. Louis, MO, USA); mouse IgG (A7028) and rabbit IgG (A7016) were purchased from Beyotime (Shanghai, China). Antibodies for γH2AX (ab22551), H2AX (ab229914), HMGB3 (ab75782), and Rad51 (ab63801) were obtained from Abcam (Cambridge, UK). Goat anti-rabbit IgG Alexa Fluor-488 (A-11008) and Goat anti-mouse IgG Alexa Fluor-594 (A-11030) for immunofluorescence staining were obtained from Invitrogen (Waltham, MA, USA). Olaparib (AZD2281) was purchased from Selleck Chemicals (Houston, TX, USA).

Antibodies against GST (10000-0-AP), poly (ADP-ribose) polymerase 1 (PARP1, 13371-1-AP), Flag (20543-1-AP), MYC-tag (16286-1-AP), GFP (66002-1-Ig), Ki-67 (27309-1-AP), PBK (16110-1-AP), XIAP (10037-1-Ig), Bcl-XL (26967-1-AP), Lamin B1 (12987-1-AP), p65 (66535-1-Ig), and caspase 3 (19677-1-AP) were purchased from Proteintech (Wuhan, China); β-actin (A5441) was obtained from Sigma-Aldrich (St. Louis, MO, USA); The mouse IgG (A7028) and rabbit IgG (A7016) were purchased from Beyotime (Shanghai, China). Antibodies for IκBα (ab32518), TRIM37 (ab264190), and GAPDH (ab8245) were obtained from Abcam (Cambridge, UK). Cleaved caspase 3 (9661) and p-IκBα (2859) were obtained from Cell Signaling Technology (Danvers, MA, USA). p-Thr (sc-5267), p-Ser (sc-81514), and p-Tyr (sc-7020) were purchased from Santa Cruz (Dallas, TX, USA). Goat anti-mouse IgG Alexa Fluor-594 (A-11030) for immunofluorescence staining was obtained from Invitrogen (Waltham, MA, USA). Olaparib (AZD2281), JSH-23 (S7652), and OTS514 (S7652) were purchased from Selleck Chemicals (Houston, TX, USA).