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2 protocols using rabbit anti v atpase tcirg1

1

Immunophenotyping and Cytotoxicity Assays

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The following reagents and Antibodies were used: Esomeprazole, 3-methylcholanthrene, DTNB, Cristal violet, Sulfasalazine and LPS (Sigma-Aldrich); sCPG (Tocris Bioscience); LysoSensor Green DND-189, BCECF-AM and CFSE (Thermo Fisher Scientific);Cisplatin (Accord Healthcare); Mouse IFN-γ, IL-4 and recombinant GM-CSF (Relia Tech GmbH), rabbit anti-human xCT, mAbs anti-human Cytokeratin 14 (LL002) and Cytokeratin 18 (LDK 18) (Abcam); rabbit anti v-ATPase (TCIRG1, Proteintech); mAb anti-human thioredoxin (clone 2B1) kindly provided by Dr F. Clarke (University of Brisbane, Australia); mAbs to human Vimentin (V9) and Smooth Muscle Actin (1A4) (Thermofisher); rat anti-mouse CD206, CD86 (AbD Serotec), F4/80, CD86-FITC, F4/80-FITC (Biolegend) and CD206-FITC (Bio-Rad Laboratories).
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2

Multicolor Flow Cytometry Analysis

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The following reagents and antibodies were used: ESO, EIPA, Crystal violet, (Sigma‐Aldrich); LysoSensor Green DND‐189, (Thermo Fisher Scientific); Cis (Accord Healthcare); rabbit anti‐v‐ATPase (TCIRG1, Proteintech); rabbit anti‐NHE‐1 and rat anti‐mouse CD11b (Novus Biologicals); rat anti‐mouse CD206 (AbD Serotec); rat anti‐mouse CD86 clone PO.3 (Millipore); rat anti‐mouse CD4, CD8, CD205 (DEC205) and Ly‐6G/Ly6C (Gr‐1) (Biolegend); rat anti‐mouse CD11b (Novus Biologicals); and rat anti‐mouse CD31(clone MEC 13.3) kindly supplied by A. Mantovani.
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