Cellevent caspase 3 7 green detection reagent caspase 3 7

Manufactured by Thermo Fisher Scientific

Sourced in United States

The CellEvent Caspase‐3/7 Green Detection Reagent is a fluorogenic substrate for the detection of caspase-3 and caspase-7 activity in live cells. It contains a four-amino acid peptide (DEVD) that is cleaved by active caspase-3 and caspase-7, releasing a green fluorescent dye that can be detected by fluorescence microscopy or flow cytometry.

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Lab products found in correlation

Bz x710 microscope, by Keyence (1 mentions) Evos microscope, by Thermo Fisher Scientific (1 mentions)

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Caspase-3 (46 citations) CellEvent (15 citations) Caspase-7 (2 citations)

3 protocols using cellevent caspase 3 7 green detection reagent caspase 3 7

Quantitative Apoptosis Imaging Analysis

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Analysis of apoptosis staining was undertaken using CellEvent Caspase‐3/7 Green Detection Reagent (Caspase 3/7) (Invitrogen). The apoptotic cells were imaged using a BZ‐X710 microscope (Keyence). Imaged data were processed and quantified using ImageJ.³⁰

Asakawa Y., Okabe A., Fukuyo M., Li W., Ikeda E., Mano Y., Funata S., Namba H., Fujii T., Kita K., Matsusaka K, & Kaneda A. (2020). Epstein‐Barr virus‐positive gastric cancer involves enhancer activation through activating transcription factor 3. Cancer Science, 111(5), 1818-1828.

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Caspase 3/7 and PI Staining of Cells Post-NB-PDT

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Right after NB-PDT, CellEvent caspase 3/7 green detection reagent (caspase 3/7) (Invitrogen, Carlsbad, CA, USA, cat no. R37111) and propidium iodide (PI) (Invitrogen, P5366) were added to the cells in a final dilution of 1:10 and 1:1000, respectively. Well plates were placed back in the incubator for 2 h and, hereafter, cells were imaged with an EVOS microscope (Thermo Fisher Scientific, Perbio Science Nederland, Etten-Leur, The Netherlands) using transmitted light, the GFP light cube for caspase 3/7, and the RFP light cube for PI (20x objective). Plates were returned to the incubator and imaged again 18 h after NB-PDT.

Beltrán Hernández I., Angelier M.L., Del Buono D’Ondes T., Di Maggio A., Yu Y, & Oliveira S. (2020). The Potential of Nanobody-Targeted Photodynamic Therapy to Trigger Immune Responses. Cancers, 12(4), 978.

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Caspase-3/7 Apoptosis Staining

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Analysis of apoptosis staining was performed using CellEvent Caspase-3/7 Green Detection Reagent (Caspase-3/7) (Invitrogen).

Sugiura M., Sato H., Okabe A., Fukuyo M., Mano Y., Shinohara K.I., Rahmutulla B., Higuchi K., Maimaiti M., Kanesaka M., Imamura Y., Furihata T., Sakamoto S., Komiya A., Anzai N., Kanai Y., Luo J., Ichikawa T, & Kaneda A. (2020). Identification of AR-V7 downstream genes commonly targeted by AR/AR-V7 and specifically targeted by AR-V7 in castration resistant prostate cancer. Translational Oncology, 14(1), 100915.

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