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Sureprint g3 mouse gene expression v2 8x60k microarray chips

Manufactured by Agilent Technologies
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The SurePrint G3 Mouse Gene Expression v2 8x60K microarray chips are designed for comprehensive analysis of mouse gene expression. These chips feature 60,000 probes that target well-annotated mouse genes, providing a thorough representation of the mouse transcriptome. The chips are part of Agilent's SurePrint G3 microarray platform, known for its high-quality design and performance.

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Lab products found in correlation

Low input quick amp labelling kit, by Agilent Technologies (1 mentions) R programming language, by Lucent Technologies (1 mentions) Cyanine3 (cy3), by Agilent Technologies (1 mentions)

2 protocols using sureprint g3 mouse gene expression v2 8x60k microarray chips

1

Microarray Analysis of Endothelial Transcriptome

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RNA was isolated from the magnetic bead isolated endothelium, reverse transcribed to cDNA, transcribed, amplified and labelled with Cy3 (Low input quick amp labelling kit, Agilent). Labelled cRNA samples were then hybridized to SurePrint G3 Mouse Gene Expression v2 8x60K microarray chips (Agilent). The R programming language (Lucent Technologies), marray (21 ) and the Limma (Bioconductor) plug-in were used to subtract background, quantile normalize probe signal intensities and perform differential gene expression analyses on the microarray data. Raw and processed data from this analysis are deposited in the Gene Expression Omnibus (GEO) repository (accession number: GSE84048).
Wragg J.W., Heath V.L, & Bicknell R. (2016). Sunitinib treatment enhances metastasis of innately drug resistant breast tumors. Cancer research, 77(4), 1008-1020.
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2

Robust Microarray Data Analysis for Gene Expression

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About 100 ng total RNA was processed for Microarray as described by manufacturer using SurePrint G3 Mouse Gene Expression v2 8x60K Microarray chips (Agilent). Microarray files were analyzed using Array studio (OmicSoft Corp. Qiagen). Data analysis includes 1) generation of raw signal intensities from g-Processed signal, 2) setting threshold/ adding constants (raw signal intensities < 5 were set as 1), 3) combining multiple probes based on Mapping gene name, 4) Log2 transformation and 5) 75th Quantile normalization. Non-parametric tests were performed on normalized data and only samples which passed QC were used for two-way ANOVA.
Escoubet J., Kenigsberg M., Derock M., Yaligara V., Bock M.D., Roche S., Massey F., de Foucauld H., Bettembourg C., Olivier A., Berthemy A., Capdevielle J., Legoux R., Perret E., Buzy A., Chardenot P., Destelle V., Leroy A., Cahours C., Teixeira S., Juvet P., Gauthier P., Leguet M., Rocheteau-Beaujouan L., Chatoux M.A., Deshayes W., Clement M., Kabiri M., Orsini C., Mikol V., Didier M, & Guillemot J.C. (2020). ABHD11, a new diacylglycerol lipase involved in weight gain regulation. PLoS ONE, 15(6), e0234780.
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