We used 8 weeks-old mice livers derived from 25 inbred mouse strains, which were kindly donated by Dr. Aldons Lusis (University of California, Los Angeles, CA, USA). (i) 129X1/SvJ, (ii) A/J, (iii) AKR/J, (iv) BALB/cJ, (v) BTBR T<+> tf/J, (vi) BUB/BnJ, (vii) C57BL/6J, (viii) C58/J, (ix) CAST/EiJ, (x) CBA/J, (xi) CE/J, (xii) DBA/2J, (xiii) KK/HlJ, (xiv) LG/J, (xv) LP/J, (xvi) MA/MyJ, (xvii) NOD/ShiLtJ, (xviii) NON/ShiLtJ, (xix) NZB/BlNJ, (xx) NZW/LacJ, (xxi) PL/J, (xxii) RIIIS/J, (xxiii) SEA/GnJ, (xxiv) SM/J, (xxv) SWR/J. Tissues were homogenized and adjusted to 50 mg tissue/mL in deionized water with a Potter-Elvehjem tissue homogenizer (Omni International, Kennesaw, GA, USA). Three or more livers per mouse strain were used to quantify traits (Supplementary Table S7 ).
Potter elvehjem tissue homogenizer
Manufactured by Omni International
Sourced in United States
The Potter-Elvehjem tissue homogenizer is a laboratory device used for the mechanical disruption and homogenization of biological tissue samples. It consists of a glass or Teflon pestle that fits inside a glass or metal tube, allowing for the controlled grinding and mixing of the sample.
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Lab products found in correlation
2 protocols using potter elvehjem tissue homogenizer
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Comprehensive Mouse Liver Profiling
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Comparative Liver Tissue Analysis in Mice
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We used 8 weeks-old mice livers derived from 27 inbred mouse strains which were kindly donated by Dr. Aldons Lusis (University of California, Los Angeles). (i) 129X1/SvJ (n=5), (ii) A/J (n=5), (iii) AKR/J (n=5), (iv) BALB/cJ (n=5), (v) BTBR T<+> tf/J (n=5), (vi) BUB/BnJ (n=3), (vii) C3H/HeJ (n=3), (viii) C57BL/6J (n=5), (ix) C58/J (n=5), (x) CAST/EiJ (n=3), (xi) CBA/J (n=5), (xii) CE/J (n=5), (xiii) DBA/2J (n=5), (xiv) FVB/NJ (n=3), (xv) KK/HlJ (n=3), (xvi) LG/J (n=4), (xvii) LP/J (n=3), (xviii) MA/MyJ (n=3), (xix) NOD/ShiLtJ (n=5), (xx) NON/ShiLtJ (n=5), (xxi) NZB/BlNJ (n=5), (xxii) NZW/LacJ (n=5), xxiii) PL/J (n=5), xxiv) RIIIS/J (n=3), xxv) SEA/GnJ (n=5), xxvi) SM/J (n=5), xxvii) SWR/J (n=5). Tissues were homogenized and adjusted to 50 mg tissue/ml in H2O, with a Potter-Elvehjem tissue homogenizer (Omni International, USA) and then stored at −80 °C until further use. This liver was selected because of its high relevance in the generation of pathophysiological phenotypes in GD [4 (link)].
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