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Tcrγδ uc7 13d5

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TCRγδ (UC7-13D5) is a mouse monoclonal antibody that recognizes the gamma-delta T cell receptor (TCRγδ) on the surface of T cells. It is designed for flow cytometry and other immunoassay applications involving the detection and analysis of TCRγδ-expressing cells.

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Lab products found in correlation

Cd11b m1 70, by BioLegend (2 mentions) Cd11c n418, by BioLegend (2 mentions) Cd19 6d5, by BioLegend (2 mentions) Ly6g 1a8, by BioLegend (2 mentions) Foxp3 fjk 16s, by Thermo Fisher Scientific (2 mentions) Cd4 gk1, by BioLegend (2 mentions) Nk1.1 pk136, by BioLegend (2 mentions) Live dead fixable violet cell stain kit, by Thermo Fisher Scientific (2 mentions) Lsrii cell analyzer, by BD (2 mentions) Accuri c6 instrument, by BD (2 mentions) Cd3ε 145d2c11, by BioLegend (2 mentions) Cd16 32 93, by Thermo Fisher Scientific (2 mentions) Rorγt b2d, by Thermo Fisher Scientific (2 mentions) Cd16 32 93, by BioLegend (2 mentions) Cd45 30 f11, by BioLegend (2 mentions) Facsdiva software, by BD (2 mentions) Siglec f, by BD (2 mentions)

Spelling variants of this product

UC7-13D5 (4 citations) Anti‐TCRγδ antibody (clone UC7‐13D5, (1 citations)

2 protocols using tcrγδ uc7 13d5

1

Multiparametric Flow Cytometry Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were isolated from the indicated tissues. Single-cell suspensions were stained with CD16/32 and with indicated fluorochrome-conjugated antibodies. Live/Dead Fixable Violet Cell Stain Kit (Invitrogen) was used to exclude non-viable cells. Multi-laser, flow cytometry analysis procedures were done at the University of Pennsylvania Flow Cytometry and Cell Sorting Facility using BD LSRII cell analyzers running FACSDiva software (BD Biosciences). Two-laser, flow cytometry analyses were done at the University of Pennsylvania iPS Cell Core using BD Accuri C6 instruments. FlowJo software (v.10 TreeStar) was used for data analysis and graphics rendering. All fluorochrome-conjugated antibodies used are listed as follows (Clone, Company, Catalog Number): CD11b (M1/70, Biolegend, 101255); CD11c (N418, Biolegend, 117318); CD16/32 (93, Biolegend, 101319); CD16/32 (93, eBiosciences, 56D0161D80); CD19 (6D5, Biolegend, 115510); CD3ε (145D2C11, Biolegend, 100304); CD4 (GK1.5, Biolegend, 100406); CD45 (30-F11, Biolegend, 103121 or 103151), CD8a (53D6.7, Biolegend, 100725); Foxp3 (FJK-16s, eBiosciences, 50-5773-82); Ly-6G (1A8, Biolegend, 127624); Live/Dead (N/A, Thermofisher, LD34966); NK1.1 (PK136, Biolegend, 108745); RORγt (B2D, eBiosciences, 12-6981-82); Siglec-F (E50D2440, BD, 562757); TCRγδ (UC7-13D5, Biolegend, 107504)
Tang A.T., Choi J.P., Kotzin J.J., Yang Y., Hong C.C., Hobson N., Girard R., Zeineddine H.A., Lightle R., Moore T., Cao Y., Shenkar R., Chen M., Mericko P., Yang J., Li L., Tanes C., Kobuley D., Võsa U., Whitehead K.J., Li D.Y., Franke L., Hart B., Schwaninger M., Henao-Mejia J., Morrison L., Kim H., Awad I.A., Zheng X, & Kahn M.L. (2017). Endothelial TLR4 and the microbiome drive cerebral cavernous malformations. Nature, 545(7654), 305-310.
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2

Multiparametric Flow Cytometry Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were isolated from the indicated tissues. Single-cell suspensions were stained with CD16/32 and with indicated fluorochrome-conjugated antibodies. Live/Dead Fixable Violet Cell Stain Kit (Invitrogen) was used to exclude non-viable cells. Multi-laser, flow cytometry analysis procedures were done at the University of Pennsylvania Flow Cytometry and Cell Sorting Facility using BD LSRII cell analyzers running FACSDiva software (BD Biosciences). Two-laser, flow cytometry analyses were done at the University of Pennsylvania iPS Cell Core using BD Accuri C6 instruments. FlowJo software (v.10 TreeStar) was used for data analysis and graphics rendering. All fluorochrome-conjugated antibodies used are listed as follows (Clone, Company, Catalog Number): CD11b (M1/70, Biolegend, 101255); CD11c (N418, Biolegend, 117318); CD16/32 (93, Biolegend, 101319); CD16/32 (93, eBiosciences, 56D0161D80); CD19 (6D5, Biolegend, 115510); CD3ε (145D2C11, Biolegend, 100304); CD4 (GK1.5, Biolegend, 100406); CD45 (30-F11, Biolegend, 103121 or 103151), CD8a (53D6.7, Biolegend, 100725); Foxp3 (FJK-16s, eBiosciences, 50-5773-82); Ly-6G (1A8, Biolegend, 127624); Live/Dead (N/A, Thermofisher, LD34966); NK1.1 (PK136, Biolegend, 108745); RORγt (B2D, eBiosciences, 12-6981-82); Siglec-F (E50D2440, BD, 562757); TCRγδ (UC7-13D5, Biolegend, 107504)
Tang A.T., Choi J.P., Kotzin J.J., Yang Y., Hong C.C., Hobson N., Girard R., Zeineddine H.A., Lightle R., Moore T., Cao Y., Shenkar R., Chen M., Mericko P., Yang J., Li L., Tanes C., Kobuley D., Võsa U., Whitehead K.J., Li D.Y., Franke L., Hart B., Schwaninger M., Henao-Mejia J., Morrison L., Kim H., Awad I.A., Zheng X, & Kahn M.L. (2017). Endothelial TLR4 and the microbiome drive cerebral cavernous malformations. Nature, 545(7654), 305-310.
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