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MSIN1 is a lab equipment product manufactured by Cell Signaling Technology. It is a tool used for the detection and analysis of cellular signaling pathways.

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Lab products found in correlation

Phospho s6 s235 236, by Cell Signaling Technology (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions) Phospho akt s473, by Cell Signaling Technology (1 mentions) Pras40, by Cell Signaling Technology (1 mentions) Deptor, by Cell Signaling Technology (1 mentions) Mlst8, by Cell Signaling Technology (1 mentions) Rictor, by Cell Signaling Technology (1 mentions) Raptor, by Cell Signaling Technology (1 mentions) C parp, by Cell Signaling Technology (1 mentions) P ndrg1 thr346, by Cell Signaling Technology (1 mentions) P pkcα ser657, by Santa Cruz Biotechnology (1 mentions) Erk1 2, by Cell Signaling Technology (1 mentions) Western lightning plus ecl, by PerkinElmer (1 mentions) Ndrg1, by Cell Signaling Technology (1 mentions) P mtor s2481, by Cell Signaling Technology (1 mentions) β actin hrp, by Santa Cruz Biotechnology (1 mentions)

2 protocols using msin1

1

Immunoblot Analysis of Cell Signaling

Cited 1 time
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All immunoblot analyses were performed as previously described [59 (link)]. Antibodies were obtained from the following sources: antibodies to phospho-T389-S6K1, phospho-S473-AKT, phospho-S235/236-S6, phospho-S65-4E-BP1, 4E-BP1 S6K1, AKT, S6, GAPDH, RICTOR, RAPTOR, DEPTOR, PRAS40, mLST8, mSIN1 were obtained from Cell Signaling Technology. Antibodies to myc (9E10) and FLAG (M2) were obtained from Sigma.
Ghosh A.P., Marshall C.B., Coric T., Shim E.H., Kirkman R., Ballestas M.E., Ikura M., Bjornsti M.A, & Sudarshan S. (2015). Point mutations of the mTOR-RHEB pathway in renal cell carcinoma. Oncotarget, 6(20), 17895-17910.
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2

NSCLC Cell Line Signaling Pathway Analysis

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Western blotting analyses were performed on total protein lysates extracted from NSCLC cell lines. Antibodies used: RICTOR, p-RICTOR (Thr1135), p-AKT (Ser473), AKT, p-MEK1/2 (Ser217/221), MEK1/2, p-p44/42 MAPK (Thr202/Tyr204) (p-ERK1/2), ERK1/2, c-PARP, PARP, p-mTOR (S2481), mTOR, p-NDRG1 (Thr346), NDRG1, mSIN1, p-4E-BP1 (Thr37/46), KRAS, p-S6RP (S235/236), S6RP are from Cell Signaling Technologies (Danvers, MA); p-PKCα(Ser657) and β-actin-HRP are from Santa Cruz Biotechnology (Santa Cruz, CA). Immunoreactivity was visualized by Western Lightning Plus-ECL (Perkin-Elmer, Waltham, MA) and exposure to x-ray film according to manufacturer. Densitometric quantification was performed using Image Studio Lite 5.0 software (Lincoln, NE).
Ruder D., Papadimitrakopoulou V., Shien K., Behrens C., Kalhor N., Chen H., Shen L., Lee J.J., Hong W.K., Tang X., Girard L., Minna J.D., Diao L., Wang J., Mino B., Villalobos P., Rodriguez-Canales J., Hanson N.E., Sun J., Miller V., Greenbowe J., Frampton G., Herbst R.S., Baladandayuthapani V., Wistuba I.I, & Izzo J.G. (2018). Concomitant targeting of the mTOR/MAPK pathways: novel therapeutic strategy in subsets of RICTOR/KRAS-altered non-small cell lung cancer. Oncotarget, 9(74), 33995-34008.
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