To evaluate the soluble factors present in the plasma of the different study groups, the Bio-Plex ProTM Human Cytokine Standard 27-plex Kit (Bio-Rad – Hercules, CA, USA) was used to measure the following molecules: cytokines (IL-1beta, IL1- Ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17, IFN-gamma, TNF) chemokines (CCL2, CCL3, CCL4, CCL5, CXCL8, CXCL10, CCL11) and growth factors (G-CSF, GM-CSF, PDGF-BB, VEGF, basic FGF). Experiments were performed according to manufacturer’s instructions. The data were acquired by the Bio-Plex 200 instrument equipped with the Manager software and the results were expressed as the mean fluorescence intensity (MFI), after background subtraction.
Bio plex protm human cytokine standard 27 plex kit
Manufactured by Bio-Rad
Sourced in United States
The Bio-Plex Pro™ Human Cytokine Standard 27-plex Kit is a multiplex assay designed to quantify 27 different human cytokines and chemokines simultaneously in a single sample. The kit utilizes magnetic bead-based technology to enable the detection and measurement of multiple analytes in a cost-effective and efficient manner.
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Lab products found in correlation
2 protocols using bio plex protm human cytokine standard 27 plex kit
1
Multiplex Cytokine Profiling in Plasma
2
Cytokine and Chemokine Profiling in Plasma
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Plasma obtained from peripheral blood as mentioned above was used to measure the following molecules: cytokines (IL1-Ra, IL-2, IL-6, IL-7, IL-10, IL-15, IL-17) and chemokines (CCL3, CCL4, CCL5) using the Bio-Plex ProTM Human Cytokine Standard 27-plex Kit (Bio-Rad—Hercules, CA, USA). Experiments were performed according to the manufacturer's instructions. The data were acquired by the Bio-Plex 200 instrument equipped with the Manager software, and the results were expressed as the mean fluorescence intensity (MFI) after background subtraction.
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